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Acta Physiologiae Plantarum

, 37:228 | Cite as

Biochemical characterization of the primary metabolism and antioxidant defense systems of acidic and acidless citrus genotypes during the major stages of fruit growth

  • Julie Oustric
  • Sandrine Antoine
  • Jean Giannettini
  • Yves Gibon
  • François Luro
  • Liliane Berti
  • Jérémie SantiniEmail author
Original Article

Abstract

Fruits are consumed not just for their taste but also for their nutritional value. The major primary metabolites in fruit are sugars and acids, whose contents change during fruit growth and determine ultimate fruit quality. Fruits are also a source of antioxidant metabolites, which are important to human health due to their role in reducing risk of cancer and cardiovascular diseases. Antioxidants are equally important in the plant as they help fight against oxidative stress. Here, we investigated the consequences of changes in the primary metabolism in acidic and acidless citrus genotypes during the major stages of fruit growth on the expression of antioxidant enzymes and the markers of cellular oxidation (hydrogen peroxide, malondialdehyde) in acidless (Iaffaoui orange and sweet lemon) and acidic (Salustiana orange and Villafranca lemon) citrus fruits. Glucose and fructose were the major sugars in the acidless lemon. Sucrose was the major sugar in the acidic lemon. Oranges shared a balance of glucose, fructose, and sucrose. Malic and citric acid concentrations were higher in acidic lemons than acidless fruits. Acidic genotypes had higher hydrogen peroxide concentrations than acidless genotypes, whereas MDA concentrations were higher in oranges than in lemons. Specific activities of ascorbate peroxidase, catalase, superoxide dismutase, and dehydroascorbate reductase were on the whole higher in acidic than acidless fruits. Principal component analysis revealed between-genotype divergence in antioxidant system, giving three groups: acidic lemons, acidless lemons, and oranges.

Keywords

Citrus Sugars Organic acids Antioxidant system Oxidative status 

Notes

Acknowledgments

The authors thank the Collectivité Territoriale de Corse (CTC) for providing financial support for this study.

Supplementary material

11738_2015_1982_MOESM1_ESM.docx (228 kb)
Supplementary material 1 (DOCX 227 kb)

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2015

Authors and Affiliations

  • Julie Oustric
    • 1
  • Sandrine Antoine
    • 1
    • 2
  • Jean Giannettini
    • 1
  • Yves Gibon
    • 3
    • 4
  • François Luro
    • 2
  • Liliane Berti
    • 1
  • Jérémie Santini
    • 1
    Email author
  1. 1.CNRS, UMR 6134 SPE, Laboratoire Biochimie and Biologie Moléculaire du VégétalCorteFrance
  2. 2.UMR AGAP Corse, Station INRASan GiulianoFrance
  3. 3.Institut National de la Recherche Agronomique and Université de Bordeaux, Unité Mixte de Recherche 1332, Biologie du Fruit et PathologieVillenave-d’OrnonFrance
  4. 4.Metabolomics Platform–Functional Genomics Centre Bordeaux, INRA BordeauxVillenave-d’OrnonFrance

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