Abstract
Using wild-type (WT) Arabidopsis thaliana plants and two transgenic lines—overexpressing alternative oxidase AOX1a (AOX1a-OE) and antisense AOX1a (AOX1a-AS)—it was observed that brassinosteroids (BRs) are involved in regulation of respiration in A. thaliana leaf, particularly alternative respiratory pathway, under salt stress conditions. This study demonstrates that BRs stimulate total respiration rate, alternative respiratory pathway, and shift capacity of alternative respiratory pathway comparing to cyanide-sensitive pathway. Lowering the level of endogenous BRs with the specific inhibitor of BRs biosynthesis—brassinazole—caused the decrease of alternative respiratory pathway activity in WT plants and transgenic AOX1a-OE and AOX1a-AS lines.
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Abbreviations
- AOX:
-
Alternative oxidase
- BL:
-
Brassinolide
- BRZ:
-
Brassinazole
- COX:
-
Cytochrome oxidase
- DW:
-
Dry weight
- EBL:
-
24-Epibrassinolide
- mETC:
-
Mitochondrial electron transport chain
- SHAM:
-
Salicylhydroxamic acid
- ROS:
-
Reactive oxygen species
- WT:
-
Wild type
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Acknowledgements
This work was supported by the State Fund for Fundamental Researches of Ukraine (grants № 41.4/041-2011, № 54.4/026-2013), NAS of Ukraine grants (№ 2.1.10.32-10, 9.1-12(06)) and Belarusian Republican Foundation for Fundamental Research (grant № X13К-094). We thank FEBS organization for its support in presentation of this material at FEBS/EMBO Course “Mitochondria in life, death and disease” (9-13 May 2012, Crete, Greece).
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Communicated by P. K. Nagar.
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Derevyanchuk, M., Litvinovskaya, R., Khripach, V. et al. Effect of 24-epibrassinolide on Arabidopsis thaliana alternative respiratory pathway under salt stress. Acta Physiol Plant 37, 215 (2015). https://doi.org/10.1007/s11738-015-1967-8
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DOI: https://doi.org/10.1007/s11738-015-1967-8