Acta Physiologiae Plantarum

, 37:190 | Cite as

Identification and characterization of a small heat shock protein 17.9-CII gene from faba bean (Vicia faba L.)

  • Ritesh Kumar
  • Dhruv Lavania
  • Amit Kumar Singh
  • Manisha Negi
  • Manzer H. Siddiqui
  • Mohamed H. Al-Whaibi
  • Anil Grover
Original Article
First Online:
Received:
Revised:
Accepted:

Abstract

We cloned and characterized the full-length coding sequence of a small heat shock protein 17.9 gene from faba bean encoding 160 amino acids and containing the conserved α-crystallin domain at the C-terminus. Homology and phylogenetic analysis suggested its proximity with the class II sHsp members of fabaceae family. Therefore, we name this gene as VfHsp17.9-CII. The VfHsp17.9-CII transcript showed a clear heat stress induction pattern in leaves of young seedlings and flowering plants. Transient expression of VfHsp17.9-CII fused with green fluorescent protein reporter indicated its nuclear localization. Overexpression of recombinant VfHsp17.9-CII protein in Escherichiacoli cells increased tolerance of the bacterial cells to heat and arsenic stresses. The reduction of faba bean pollen viability in response to heat stress correlated with the accumulation pattern of VfHsp17.9-CII transcript in heat stressed pollen. It is suggested that VfHsp17.9-CII protein plays a key role in heat and heavy metal stress tolerance.

Keywords

Faba bean Heat stress Nuclear localization Pollen Small heat shock protein Transcript induction 

Notes

Acknowledgments

RK is thankful to University Grants Commission, India for fellowship. DL is thankful to Council of Scientific and Industrial Research, Government of India for the research fellowship award. MHS and MHA-W thank project funding from National Plan for Science and Technology Program, Saudi Arabia (Project No. 11-BIO1922-02). AG gratefully acknowledges Visiting Professorship of King Saud University, Saudi Arabia.

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2015

Authors and Affiliations

  • Ritesh Kumar
    • 1
  • Dhruv Lavania
    • 1
  • Amit Kumar Singh
    • 1
  • Manisha Negi
    • 1
  • Manzer H. Siddiqui
    • 2
  • Mohamed H. Al-Whaibi
    • 2
  • Anil Grover
    • 1
  1. 1.Department of Plant Molecular BiologyUniversity of DelhiNew DelhiIndia
  2. 2.Department of Botany and MicrobiologyKing Saud UniversityRiyadhSaudi Arabia

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