Skip to main content
SpringerLink
Log in

Gene expression and morphological characterization of cell suspensions of Coffea arabica L. cv. Catiguá MG2 in different cultivation stages

  • Original Article
  • Published:
Acta Physiologiae Plantarum Aims and scope Submit manuscript

Abstract

The present study aimed to evaluate the quality of cell suspension of Coffea arabica cv. Catiguá at four different ages by morphological and gene expression analysis. For this purpose, cell suspension samples were collected at 30, 45, 60 and 75 days. Morphological analysis revealed the presence of embryogenic regions in all samples, and after 60 days, non-embryogenic regions were also identified. The genic analyses revealed that as the cells were transferred to a new medium a change in both their physical and chemical conditions was noticed that caused stress. The decrease in SERK and BBM genes expression after 75th day may also be due to the non-embryogenic regions, characterized by large, elongated and vacuolated cells that were observed in the periphery of embryogenic regions, starting from 60 days of culture.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

Abbreviations

MG2:

Minas gerais 2

ECS:

Embryogenic cell suspensions

SERK :

Somatic embryogenesis receptor-like kinase

BBM :

Baby Boom

2,4-d :

2,4-Dichlorophenoxyacetic acid

IBA:

Indole-3-butyric acid

2-iP:

2-Isopentenyladenine

qPCR:

Real-time quantitative PCR

PCR:

Polymerase chain reaction

RNA:

Ribonucleic acid

TAE:

Tris–acetate–EDTA

DNAse:

Deoxyribonuclease

cDNA:

Complementary DNA

ml:

Milliliters

μg:

Microgram

ng:

Nanogram

µM:

Micromolar

GAPDH:

Glyceraldehyde 3-phosphate dehydrogenase

References

  • Barsalobres-Cavallari CF, Severino FE, Maluf MP, Maia IG (2009) Identification of suitable internal control genes for expression studies in Coffea arabica under different experimental conditions. BMC Mol Biol 10:1–11. doi:10.1186/1471-2199-10-1

    Article  PubMed Central  PubMed  Google Scholar 

  • Boutilier K, Offringa R, Sharma V, Kieft H, Ouellet T, Zhang L, Hattori J, Liu C, Van Lammeren A, Miki B, Custers J, Campagne M (2002) Ecotopic expression of BABY BOOM triggers a conversion from vegetative to embryonic growth. Plant Cell 14:1737–1749. doi:10.1105/tpc.001941

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Carvalho JMFC, González-Benito E, Perez C (2001) Análise histológica de calogênese e embriogênese das cultivares de algodão cnpa precoce 2 e coker 312. Rev Fibros 5:235–239

    Google Scholar 

  • Ferreira MGR, Carvalho CHS, Carneiro AA, Damião-Filho CF (2005) Indução de embriogênese somática em cupuaçu (Theobroma grandiflorum Schum). Revista Brasileira de Fruticultura Jaboticabal 3:500–503

    Article  Google Scholar 

  • Gatica-Arias AM, Arrieta-Espinoza G, Esquivel AME (2008) Plant regeneration via indirect somatic embryogenesis and optimisation of genetic transformation in Coffea arabica L. cvs. Caturra and Catuaí. Electron J Biotechnol 11:1–12. doi: 10.2225/vol11-issue1-fulltext-9

    Article  Google Scholar 

  • George EF, Hall MA, Klerk GJ (2008) Plant propagation by tissue culture, 3rd edn. The Background, Dordrecht, p 501

    Google Scholar 

  • Guerra PG, Torres AC, Teixeira JB (1999) Embriogênese somática e sementes sintéticas. In: Torres AC, Caldas LS, Buso LA (eds) Cultura de tecidos e transformação genética de plantas, vol 2. Embrapa, Brasilia, pp 533–568

    Google Scholar 

  • Heidmann I, Lange B, Lambalk J, Angenent GC, Boutilier K (2011) Efficient sweet pepper transformation mediated by the BABY BOOM transcription factor. Plant Cell Rep 30:1107–1115. doi:10.1007/s00299-011-1018-x

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Mahdavi-Darvari F, Noor NM, Ismanizan I (2015) Epigenetic regulation and gene markers as signals of early somatic embryogenesis. Plant Cell Tissue Organ Cult 120:407–422. doi:10.1007/s11240-014-0615-0

    Article  CAS  Google Scholar 

  • Morais TP, Melo B (2011) Biotecnologia aplicada ao melhoramento genético do cafeeiro. Ciência Rural 5:753–760

    Article  Google Scholar 

  • Morais H, Marur CJ, Caramori PH, Ribeiro AMA, Gomes JC (2003) Características fisiológicas e de crescimento de cafeeiros sombreados com guandu e cultivados a pleno sol. Pesquisa Agropecuária Brasileira 10:1131–1137

    Google Scholar 

  • Moura EF, Ventrella MC, Motoike SY, Sá AQJR, Carvalho M, Manfio CE (2008) Histological study of somatic embryogenesis induction on zygotic embryos of macaw palm (Acrocomia aculeata (Jacq.) Lodd. ex Martius). Plant Cell Tissue Organ Cult 95:175–184. doi:10.1007/s11240-008-9430-9

    Article  Google Scholar 

  • Oliveira AC, Pereira AA (2014) CaféPoint. Disponível em: http://www.cafepoint.com.br/radares-tecnicos/variedades-de-cafe/cultivares-de-cafe-arabica-desenvolvidas-pela-epamig-47444n.aspx. Accessed em 17 May 2014

  • Quiroz-Figueroa FR, Fuentes-Cerda CFJ, Rojas-Herrera R, Loyola-Vargas VM (2002) Histological studies on the developmental stages and differentiation of two different somatic embryogenesis systems of Coffea Arabica. Plant Cell Rep 20:1141–1149. doi:10.1007/s00299-002-0464-x

    Article  CAS  Google Scholar 

  • Ribas AF, Dechamp E, Champion A, Bertrand B, Combes MC, Verdeil J, Lapeyre F, Lashermes P, Etienne H (2011) Agrobacterium-mediated genetic transformation of Coffea arabica (L.) is greatly enhanced by using established embryogenic callus cultures. BMC Plant Biol 11:92. doi:10.1186/1471-2229-11-92

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Samson NP, Campa C, Le Gal L, Noirot M, Thomas G, Lokeswari TS, Kochko AD (2006) Effect of primary culture medium composition on high frequency somatic embryogenesis in different Coffea species. Plant Cell Tissue Organ Cult 86:37–45. doi:10.1007/s11240-006-9094-2

    Article  Google Scholar 

  • Santos MO, Aragão FJL (2009) Role of SERK genes in plant environmental response. Plant Signal Behav 4:1111–1113. doi:10.4161/psb.4.12.9900

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Savona M, Mattioli R, Nigro S, Falasca G, Della Rovere F, Costantino P, De Vries S, Ruffoni B, Trovato M, Altamura MM (2011) Two SERK genes are markers of pluripotency in Cyclamen persicum Mill. J Exp Bot 63:471–488. doi:10.1093/jxb/err295

    Article  PubMed  Google Scholar 

  • Schellenbaum P, Jacques A, Maillot P (2008) Characterization of VvSERK1, VvSERK2, VvSERK3, and VvLIL genes and their expression during somatic embryogenesis of grapevine (Vitis vinifera L.). Plant Cell Rep 27:1799–1809. doi:10.1007/s00299-008-0588-8

    Article  CAS  PubMed  Google Scholar 

  • Schmidt ED, Guzzo F, Toonen MA, De Vries SC (1997) A leucine-rich repeat containing receptor-like kinase marks somatic plant cells competent to form embryos. Development 124:2049–2062

    CAS  PubMed  Google Scholar 

  • Silva AT (2011) Análise da expressão dos genes baby boom (BBM) e somatic embryogenesis receptor kinase (SERK) envolvidos na embriogênese somática do cafeeiro. 2011. 132 f. Dissertação (Mestrado em Biotecnologia Vegetal). Universidade Federal de Lavras, Lavras

  • Silva AT, Barduche D, Livramento KG, Ligterink W, Paiva LV (2013) Characterization of a putative Serk-like ortholog in embryogenic cell suspension cultures of Coffea arabica L. Plant Mol Biol Rep 32:176–184. doi:10.1007/s11105-013-0632-x

    Article  Google Scholar 

  • Stein VC et al (2010) Ultrastructural calli analysis of Inga vera Willd. Rev Árvore 34:789–796

    Article  Google Scholar 

  • Steinmacher DA, Guerra MP, Saare-Surminski K, Lieberei R (2011) A temporary immersion system improves in vitro regeneration of peach palm through secondary somatic embryogenesis. Ann Bot 108:1463–1475. doi:10.1093/aob/mcr033

    Article  PubMed Central  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The authors would like to thank Fundação de Amparo à Pesquisa de Minas Gerais (FAPEMIG), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for their financial support.

Author information

Authors and Affiliations

  1. Departamento de Química/Laboratório Central de Biologia Molecular, Universidade Federal de Lavras, Campus Universitário, Lavras, MG, CEP 37200-000, Brazil

    Luana Ferreira Torres, Kalynka Gabriella Do Livramento, Luciana Lima Freire & Luciano Vilela Paiva

  2. Embrapa Tabuleiros Costeiros/Universidade Tiradentes, Avenida Beira Mar 3250, Bairro Jardins, Aracaju, SE, CEP 49025-040, Brazil

    Leandro Eugenio Cardamone Diniz

Authors
  1. Luana Ferreira Torres
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Leandro Eugenio Cardamone Diniz
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kalynka Gabriella Do Livramento
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Luciana Lima Freire
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Luciano Vilela Paiva
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Luciano Vilela Paiva.

Additional information

Communicated by Y. Wang.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Torres, L.F., Diniz, L.E.C., Do Livramento, K.G. et al. Gene expression and morphological characterization of cell suspensions of Coffea arabica L. cv. Catiguá MG2 in different cultivation stages. Acta Physiol Plant 37, 175 (2015). https://doi.org/10.1007/s11738-015-1924-6

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s11738-015-1924-6

Keywords

Search

Navigation