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Salicylic acid alleviates the heat stress-induced oxidative damage of starch biosynthesis pathway by modulating the expression of heat-stable genes and proteins in wheat (Triticum aestivum)

  • Ranjeet R. KumarEmail author
  • Sushil K. Sharma
  • Suneha Goswami
  • Pooja Verma
  • Khushboo Singh
  • Nidhi Dixit
  • Himanshu Pathak
  • C. Viswanathan
  • Raj D. Rai
Original Article

Abstract

High temperature reduces the growth and yield of most of the agriculturally important crops. Elicitors have been reported to modulate the thermotolerance of crops under abiotic stresses. Here, we studied the effect of salicylic acid (SA) on thermotolerance level of C306 (thermotolerant) and PBW343 (thermosusceptible) wheat cultivars grown under heat stress. Pilot experiment confirmed spraying of 100 mM SA prior to heat stress (38 °C, 2 h) as the most effective treatment. Numerous protein spots were observed in C306 under SA + HS compared to PBW343 during post-anthesis stage by 2-DE. Differentially expressed proteins were identified as signaling molecule, heat-responsive transcription factors (HSFs) and heat shock proteins (HSPs) using MALDI-TOF–TOF/MS analysis. Abundance of transcripts of HSFs, HSPs, CDPK, SOD, RCA, etc. was observed in C306 in response to SA + HS, as compared to PBW343. C306 showed better accumulation of transcript of SAGs, osmolyte and total antioxidant capacity under SA treatment compared to PBW343. SA was observed to reduce the detrimental effect of HS on soluble starch synthase (SSS) activity as well as synthesis of starch granules in both the cultivars.

Keywords

Anthesis CDPK Elicitor Heat stress HSFs HSPs qRT-PCR Rubisco activase Salicylic acid Starch synthase Starch Transcript 

Notes

Acknowledgments

The financial support received from Indian Council of Agriculture Research (ICAR) under the National Initiative for Climate Resilient Agriculture (NICRA) project (12/115 TG 3079) and extra-mural research funding  by SERB, Department of Science and Technology (DST) (Project  no. SERB/SB/SO/PS/07/2014) is highly acknowledged.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11738_2015_1899_MOESM1_ESM.docx (440 kb)
Supplementary material 1 (DOCX 439 kb)

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2015

Authors and Affiliations

  • Ranjeet R. Kumar
    • 1
    Email author
  • Sushil K. Sharma
    • 1
  • Suneha Goswami
    • 1
  • Pooja Verma
    • 1
  • Khushboo Singh
    • 1
  • Nidhi Dixit
    • 1
  • Himanshu Pathak
    • 2
  • C. Viswanathan
    • 3
  • Raj D. Rai
    • 1
  1. 1.Division of BiochemistryIndian Agricultural Research Institute (IARI)New DelhiIndia
  2. 2.CESCRAIndian Agricultural Research Institute (IARI)New DelhiIndia
  3. 3.Division of Plant PhysiologyIndian Agricultural Research Institute (IARI)New DelhiIndia

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