Physiology of salinity tolerance in Aegilops cylindrica

Original Paper

Abstract

Aegilops cylindrica species is one of the valuable gene pool of wheat for the understanding of salinity-tolerance mechanisms such as Na+ exclusion. Eighty-eight Ae. cylindrica genotypes were collected from saline and non-saline areas of West Iran and used in this study. Physiological and morphological traits including shoot and root fresh and dry weights, leaf MDA and H2O2 contents, leaf and root Na+, K+ and Ca2+ concentrations, K+/Na+ and Ca2+/Na+ ratio of leaves and salinity tolerance index were evaluated. Salinity stress caused significant increases in MDA and H2O2 content, Na+, Ca2+ concentrations of root and leaves, while it led to significant decline in the remaining traits. Although dry matter correlated with leaf K+/Na+ ratio (R2 = 0.48), the regression coefficient was higher for leaf Na+ concentration (R2 = 0.58). The results of principal component analysis revealed two components (PC1 and PC2) which totally justified 52.47 and 48.02 % of total variations of the traits in control and salinity stress conditions, respectively. Three hypersalinity-tolerant genotypes originating from the shore areas resulted from shrinking of Uremia Salt Lake and depicted by the highest PC1, PC2, dry shoot weight and leaf K+/Na+ ratio as well as the lower Na+ concentration in leaves and roots. The high Na+ exclusion ability in roots and shoots of Ae. cylindrica genotypes open up new avenues for further analyses at the cellular and molecular levels to address the role of C genome as well as the complex relations between C and D genomes to cope with hypersalinity stress via ionic homeostasis.

Keywords

Aegilops cylindrica Sodium exclusion Salt tolerance Wheat breeding 

Abbreviations

DW

Dry weight

FW

Fresh weight

STI

Salt tolerance index

Supplementary material

11738_2015_1881_MOESM1_ESM.doc (178 kb)
Supplementary material 1 (DOC 178 kb) Supplementary Table S1. Origin of 88 Aegilops cylindrica genotypes used in this study including collection site, latitude, longitude, and altitude

References

  1. Anschutz U, Becker D, Shabala S (2014) Going beyond nutrition: regulation of potassium homoeostasis as a common denominator of plant adaptive responses to environment. J Plant Physiol 171:670–687PubMedCrossRefGoogle Scholar
  2. Arabbeigi M, Arzani A, Majidi MM, Kiani R, Tabatabaei BES, Habibi F (2014) Salinity tolerance of Aegilops cylindrica genotypes collected from hyper-saline shores of Uremia Salt Lake using physiological traits and SSR markers. Acta Physiol Plant 36:2243–2251CrossRefGoogle Scholar
  3. Arzani A (2008) Improving salinity tolerance in crop plants: a biotechnological view. In Vitro Cell Dev Biol Plant 44:373–383CrossRefGoogle Scholar
  4. Byrt C, Xu B, Krishnan M (2014) The Na+ transporter, TaHKT1; 5-D, limits shoot Na+ accumulation in bread wheat. Plant J 80:516–526PubMedCrossRefGoogle Scholar
  5. Chen L, Ren J, Shi H, Chen X, Zhang M, Pan Y, Fan J, Nevo E, Sun D, Fu J, Peng J (2013) Physiological and molecular responses to salt stress in wild emmer and cultivated wheat. Plant Mol Biol Rep 31:1212–1219CrossRefGoogle Scholar
  6. Colmer TD, Flowers TJ, Munns R (2006) Use of wild relatives to improve salt tolerance in wheat. J Exp Bot 57:1059–1078PubMedCrossRefGoogle Scholar
  7. Colquhoun J, Fandrich L (2003) Jointed goatgrass Aegilops cylindrica Host, vol 256. Oregon State University, PNW, pp 1–4Google Scholar
  8. Cuin TA, Betts SA, Chalmandrier R, Shabala S (2008) A root’s ability to retain K+ correlates with salt tolerance in wheat.  J Exp Bot 59:2697–2706PubMedCentralPubMedCrossRefGoogle Scholar
  9. Davenport RJ, Munoz-Major A, Jha D, Essah PA, Rus A, Tester M (2007) The Na+ transport Athkt 1:1 controls retrieval of Na+ from the xylem tn arabidopsis. Plant Cell Environ 30:497–507PubMedCrossRefGoogle Scholar
  10. Dubcovsky J, Santa-Maria G, Epstein E, Luo MC, Dovrak J (1996) Mapping of K+/Na+ discrimination locus Kna1 in wheat. Theor Appl Genet 92:448–454PubMedCrossRefGoogle Scholar
  11. Dvorak J, Noaman MM, Goyai S, Gorham J (1994) Enhancement of the salt tolerance of Triticum turgidum L. by the Knal locus transferred from the Triticum aestivum L. chromosome 4D by homoeologous recombination. Theor Appl Genet 87:872–877PubMedCrossRefGoogle Scholar
  12. Farooq S, Asghar M, Askari E, Shah TM (1994) Production and evaluation of salt tolerant wheat germplasm derived through crosses between wheat (Triticum aestivum L.) and Aegilops cylindrica. I. Production of salt tolerant wheat germplasm. Pak J Bot 26:283–292Google Scholar
  13. Fernandez GCJ (1993) Effective selection criteria for assessing plant stress tolerance. In: Kuo CG (ed) Adaptation of food crops to temperature and water stress. AVRDC, Shanhua, pp 257–270Google Scholar
  14. Golabadi M, Arzani A, Mirmohammadi Maibody SAM (2006) Assessment of drought tolerance in segregating populations in durum wheat. Afr J Agric Res 1:162–171Google Scholar
  15. Gorham J (1990) Salt tolerance in the Triticeae: K+/Na+ discrimination in Aegilops species. J Exp Bot 41:615–621CrossRefGoogle Scholar
  16. Gorham J (1994) Salt tolerance in the Triticeae: K+/Na+ discrimination in some perennial wheatgrasses and their amphiploids with wheat. J Exp Bot 45:441–447CrossRefGoogle Scholar
  17. Hoagland DR, Arnon DI (1950) The water-culture method for growing plants without soil. Calif Agric Exp Stat Circul 374:1–32Google Scholar
  18. Houshmand S, Arzani A, Maibody SAM, Feiz M (2005) Evaluation of salt-tolerant genotypes of durum wheat derived from in vitro and field experiments. Field Crops Res 91:345–354CrossRefGoogle Scholar
  19. Imlay JA, Linn S (1988) DNA damage and oxygen radical toxicity. Science 240:1302–1309PubMedCrossRefGoogle Scholar
  20. Johanson RA, Wichern DW (2007) Applied multivariate statistical analysis. Prentice Hall Inter Inc, New JerseyGoogle Scholar
  21. Jones RGW, Storey R (1978) Salt stress and comparative physiology in the Gramineae. IV. Comparison of salt stress in Spartina townsendii and three barley cultivars. Aust J Plant Physiol 5:839–850CrossRefGoogle Scholar
  22. Kim SY, Lim JH, Park MR, Kim YJ, Park TH, Sco YW, Choi KG, Yun SJ (2005) Enhanced antioxidant enzymes are associated with reduced hydrogen peroxide in barley roots under saline stress. J Biochem Mol Biol 38:218–224PubMedCrossRefGoogle Scholar
  23. Munns R, Tester M (2008) Mechanisms of salinity tolerance. Annu Rev Plant Biol 59:651–681PubMedCrossRefGoogle Scholar
  24. Munns R, Rebetzke GJ, Husain S, James RA, Hare RA (2003) Genetic control of sodium exclusion in durum wheat. Aust J Agric Res 54:627–635CrossRefGoogle Scholar
  25. Munns R, James RA, Uchli AL (2006) Approaches to increasing the salt tolerance of wheat and other cereals. J Exp Bot 57:1025–1043PubMedCrossRefGoogle Scholar
  26. Munns R, James RA, Xu B, Athman A, Conn SJ, Jordans C, Byrt CS, Hare RA, Tyerman SD, Tester M, Plett D, Gilliham M (2012) Wheat grain yield on saline soils is improved by an ancestral transporter gene. Nat Biotechnol 30:360–364PubMedCrossRefGoogle Scholar
  27. Murakeozy EP, Nagy Z, Duhaze C, Bouchereau A, Tuba Z (2003) Seasonal changes in the levels of compatible osmolytes in three halophytic species of inland saline vegetation in Hungary. J Plant Physiol 160:395–401PubMedCrossRefGoogle Scholar
  28. Pottosin I, Shabala S (2014) Polyamines control of cation transport across plant membranes: implications for ion homeostasis and abiotic stresss signaling. Plant Sci 5:1–16Google Scholar
  29. Pritchard J, Jones RGW, Tomos AD (1991) Turgor, growth, and rheological gradients of wheat roots following osmotic stress. J Exp Bot 42:1043–1049CrossRefGoogle Scholar
  30. Sairam RK, Srivastava GC, Agarwal S, Meena RC (2005) Differences in antioxidant activity in response to salinity stress in tolerant and susceptible wheat genotypes. Biol Plant 49:85–91CrossRefGoogle Scholar
  31. SAS Institute (2011) Base SAS 9.3 procedures guide. SAS Institute Inc, CaryGoogle Scholar
  32. Shabala S, Hariadia Y, Jacobsen SE (2013) Genotypic difference in salinity tolerance in quinoa is determined by differential control of xylem Na+ loading and stomatal density. J Plant Physiol 170:906–914PubMedCrossRefGoogle Scholar
  33. Shabala S, Pottosin I (2014) Regulation of potassium transport in plant under hostile conditions: implications for abiotic and biotic stress tolerance. Physiol Plant 151:257–279PubMedCrossRefGoogle Scholar
  34. Talei D, Valdiani A, Yusop MK, Abdullah MP (2013) Estimation of salt tolerance in Andrographis paniculata accessions using multiple regression model. Euphytica 189:147–160CrossRefGoogle Scholar
  35. Taulavuori E, Hellstro MEK, Taulavuori K (2001) Comparison of two methods used to analyse lipid peroxidation from Vaccinium myrtillus (L.) during snow removal, reacclimation and cold acclimation. J Exp Bot 52:2375–2380PubMedCrossRefGoogle Scholar
  36. Velikova V, Yordanov I, Edreva A (2000) Oxidative stress and some antioxidant systems in acid rain treated bean plants. Protective role of exogenous polyamines. Plant Sci 151:59–66CrossRefGoogle Scholar
  37. Zeng L, Kwon TR, Liu X, Wilson C, Grieve CM, Gregorio GB (2004) Genetic diversity analyzed by microsatellite markers among rice (Oryza sativa L.) genotypes with different adaptation to saline soils. Plant Sci 166:1275–1285CrossRefGoogle Scholar
  38. Zhu M, Shabala S, Shabala L, Fan Y, Zhou MX (2015) Evaluating predictive values of various physiological indices for salinity stress tolerance in wheat. J Agron Crop Sci. doi:10.1111/jac.12122 Google Scholar

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2015

Authors and Affiliations

  1. 1.Department of Agronomy and Plant Breeding, College of AgricultureIsfahan University of TechnologyIsfahanIran
  2. 2.Department of Plant SciencesUniversity of CaliforniaDavisUSA
  3. 3.Department of Agronomy, Miandoab BranchIslamic Azad UniversityMiandoabIran

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