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Identification of six mitogen-activated protein kinase (MAPK) genes in banana (Musa acuminata L. AAA group, cv. Cavendish) under infection of Fusarium Oxysporum f. sp cubense Tropical Race 4

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Abstract

Mitogen-activated protein kinases (MAPKs) play important roles in signal transduction pathways responding to various stresses. In this study, we described the molecular characteristics of six MAPK genes (MaMAPK1, MaMAPK2, MaMAPK3, MaMAPK4, MaMAPK5 and MaMAPK6) cloned from banana (Musa acuminate L. AAA group, cv. Cavendish) using a RACE-PCR-based strategy. The predicted molecular masses of these MAPKs ranged from 43.0 to 70.1 kDa and their pIs ranged from 5.67 to 9.32. At the amino acid level, they shared high sequence similarity with MAPKs in the banana DH-Pahang (AA group) genome. Phylogenetic analysis showed that the deduced amino acid sequences of MaMAPKs also had high similarity with MAPKs of other plant species. These genes were expressed in nearly all tissues using semi-quantitative RT-PCR. All of the six MaMAPKs were induced by signal molecules in banana seedlings, such as ABA, Ethephon, MeJA and SA. The gene expressions of MaMAPK1, MaMAPK2, MaMAPK3, MaMAPK5 and MaMAPK6 were induced in resistant cultivar of banana after being inoculated with Fusarium Oxysporum f specialis (f. Sp) cubense Tropical Race 4 (Foc TR4). Our results suggest that MaMAPKs play a key role in both the development of banana seedlings and banana resistance to Foc TR4.

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Acknowledgments

This work was supported by the Ministry of Science and Technology of the People’s Republic of China (No. 2011AA10020605) and the earmarked funds for Modern Agro-industry Technology Research System of China (CARS-32).

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Authors and Affiliations

  1. Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Hainan, 571101, People’s Republic of China

    Zhuo Wang, Caihong Jia, Jianpin Li, Biyu Xu & Zhiqiang Jin

  2. Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Hainan, 570101, People’s Republic of China

    Zhiqiang Jin

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  1. Zhuo Wang
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  2. Caihong Jia
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  3. Jianpin Li
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  4. Biyu Xu
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Correspondence to Zhuo Wang.

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Communicated by M. Stobiecki.

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Wang, Z., Jia, C., Li, J. et al. Identification of six mitogen-activated protein kinase (MAPK) genes in banana (Musa acuminata L. AAA group, cv. Cavendish) under infection of Fusarium Oxysporum f. sp cubense Tropical Race 4. Acta Physiol Plant 37, 115 (2015). https://doi.org/10.1007/s11738-015-1868-x

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  • DOI: https://doi.org/10.1007/s11738-015-1868-x

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