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Camptothecin from Nothapodytes nimmoniana: review on biotechnology applications

Abstract

Since the first report on Camptothecin detection in Nothapodytes nimmoniana by Govindachari and Viswanathan (Phytochem 11:35–29, 1972), considerable work has been done on biotechnology and its applications on the species. Plant tissue culture techniques have applications in clonal propagation, CPT production, and conservation of N. nimmoniana. Discovery of CPT production by endophytes existing in symbiotic association with N. nimmoniana has provided new insights into finding alternative sources of the alkaloid. Development of molecular markers such as RFLP, RAPD, ISSR, and AFLP has facilitated understanding of population ecology and genetics of the species. Molecular information generated from these studies is promising in establishing strategies for conservation and sustainable use of N. nimmoniana populations under overexploitation pressure. The advances in instrumentation in the 20th century, such as desorption electrospray ionization mass spectrometry allowed CPT analysis in tissues without sample pretreatment. Other ancient techniques for qualitative and quantitative analysis such as chromatography, spectroscopy, and H1-NMR are applied in the detection of CPT due to variable sensitivity to the alkaloid. The review covers work on plant tissue culture for clonal propagation and CPT production in N. nimmoniana. Besides symbiotic endophyte sources of CPT in N. nimmoniana, population genetics studies and instrumentation analysis of the alkaloids are reviewed.

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Abbreviations

CPT:

Camptothecin

9-Met. CPT:

9-Methoxycamptothecin

MS:

Murashige and skoog

PGRs:

Plant growth regulators

BAP:

Benzylaminopurine

2,4-D:

2,4-Dichlorophenoxyacetic acid

IBA:

Indole 3-butyric acid

NAA:

Naphthalene acetic acid

GA3 :

Gibberellic acid

TCP:

Tri-calcium phosphate

MEP:

C-Methyl d-erythritol-4-phosphate pathway

IPP:

Isopentenyl pyrophosphate

DMAPP:

Dimethyl allyl pyrophosphate

CD:

Czapek dox

ARDRA:

Ribosomal DNA restriction analysis

CTAB:

Cetyl trimethylammonium bromide

RFLP:

Restriction fragment length polymorphism

RAPD:

Random amplified polymorphic DNA

SSR:

Simple sequence repeat interval

AFLP:

Amplified fragment length polymorphism

PCA:

Principal coordinate analysis

UPGMA:

Unweighted pair group method with arithmetic

MAE:

Microwave-assisted extraction

DMSO:

Dimethylsulfoxide

SFET:

Supercritical fluid extraction technique

ASE:

Accelerated solvent extractor

MEKC:

Micellar electrokinetic chromatography

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Acknowledgments

This work is supported by funding to Tasiu Isah from Department of Biotechnology, Government of India New Delhi and Third World Academy of Science (TWAS) Trieste Italy, through DBT—TWAS Postgraduate Research Fellowship. We are highly grateful to Hamdard University New Delhi, India for providing research facilities. We also thank anonymous reviewers for their constructive comments on the manuscript.

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Correspondence to Tasiu Isah.

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Communicated by A. K. Kononowicz.

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Isah, T., Mujib, A. Camptothecin from Nothapodytes nimmoniana: review on biotechnology applications. Acta Physiol Plant 37, 106 (2015). https://doi.org/10.1007/s11738-015-1854-3

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Keywords

  • Biotechnology
  • Nothapodytes nimmoniana
  • Alkaloids
  • In vitro culture
  • Camptothecin
  • Endophytes