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Comparative proteomic analysis of leaves, leaf sheaths, and roots of drought-contrasting sugarcane cultivars in response to drought stress

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Abstract

A better understanding of drought response proteins may improve our understanding of the mechanisms underlying drought tolerance in sugarcane. In this research, drought-tolerant (K86-161) and drought-sensitive (B34-164) sugarcane cultivars were grown and exposed to drought stress. The changes in protein expression in leafs, leaf sheaths and roots were analyzed using proteomics techniques. Proteins that responded to drought in both cultivars could be classified into four major categories, including energy and metabolism, photosynthesis, antioxidant, and defense protein. Interestingly, an increased abundance of fructose-bisphosphate aldolase under drought was observed in all three organs of K86-161. Elevated expression of oxygen-evolving enhancer protein was also found in leaves and leaf sheaths of K86-161, when compared with their controls. Additionally, SOD was abundant in the leaves and roots of K86-161. Importantly, the expression level of these proteins decreased in B34-164 under drought stress. These contrasting results suggest that these proteins were inhibited by drought stress in the drought-sensitive cultivar. This proteomic research is the first to combine analyses of leaves, leaf sheaths and roots in sugarcane, which may enhance our understanding of drought responses at the molecular level and lead to selective breeding for enhanced drought tolerance.

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Abbreviations

2D-PAGE:

Two-dimensional polyacrylamide gel electrophoresis

LC/MS-MS:

Liquid chromatography coupled to mass spectrometry

FC:

Field capacity

PWC:

Permanent wilting point

IEF:

Isoelectric focusing

PRK:

Phosphoribulokinase

FNR:

Ferredoxin-NADP reductase

RuBP:

d-Ribulose 1,5-bisphosphate

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

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Acknowledgments

This study was financially supported by the National Research University Project through the Bio-Fuel Research Cluster and Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Department of Biochemistry, Faculty of Science, Khon Kaen University. This study was supported (in part) by Northeast Thailand Cane and Sugar Research Center Khon Kaen University. We are most grateful to Mr. Werapon Ponragdee, Khon Kaen Field Crop Research Center, for providing sugarcane cultivars.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand

    Siriporn Khueychai, Sakda Daduang, Khomsorn Lomthaisong & Sompong Klaynongsruang

  2. Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Khon Kaen University, Khon Kaen, 40002, Thailand

    Siriporn Khueychai, Nisachon Jangpromma, Sakda Daduang, Khomsorn Lomthaisong & Sompong Klaynongsruang

  3. Office of the Dean, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand

    Nisachon Jangpromma

  4. Northeast Thailand Cane and Sugar Research Center, Khon Kaen University, Khon Kaen, 40002, Thailand

    Prasit Jaisil

  5. Faculty of Humanities and Social Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand

    Apisak Dhiravisit

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  1. Siriporn Khueychai
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  2. Nisachon Jangpromma
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  3. Sakda Daduang
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  6. Apisak Dhiravisit
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Correspondence to Sompong Klaynongsruang.

Additional information

Communicated by M. Hajduch.

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11738_2015_1826_MOESM1_ESM.tif

Supplementary Fig. 1 Experimental design; the sixteen trees used for the experiments were randomly sampled from a heterogeneous population. The greenhouse conditions were used for growing the 2 sugarcane cultivars, K86-161 and B34-164 randomized by technical stages of stress to avoid instrumental drift and differences between batches. The white square indicates the well-watered control and gray squares indicate the drought-stressed plants for sugarcane K86-161 cultivar, respectively. The white triangles indicate well-watered (TIFF 3253 kb)

11738_2015_1826_MOESM2_ESM.tif

Supplementary Fig. 2 The replication of K86-161 and B34-164 sugarcane cultivars 2D gel. (a) and (b) show 2D- in leaf from well-watered control and drought-stressed K86-161 cultivar, respectively. (c) and (d) show 2D- in leaf from well-watered control and drought-stressed B34-164 cultivar, respectively. (e) and (f) show 2D- in leaf sheath from well-watered control and drought-stressed K86-161 cultivar, respectively. (g) and (h) show 2D- in leaf sheath from well-watered control and drought-stressed B34-164 cultivar, respectively. (i) and (j) show 2D- in root from well-watered control and drought-stressed K86-161cultivar, respectively. (k) and (l) show 2D- in root from well-watered control and drought-stressed B34-164 cultivar, respectively (TIFF 16309 kb)

11738_2015_1826_MOESM3_ESM.docx

Supplementary Table 1. Information about matched peptide sequence, changes in expression and organism sources of drought-responsive protein in leaves, leaf sheaths and root of the K86-161 and B34-164 cultivars that were identified by LC/MS–MS (DOCX 482 kb)

11738_2015_1826_MOESM4_ESM.xlsx

Supplementary Table 2 The relative volumes of all protein spots responding to drought in leaves, leaf sheaths and root of the K86-161 and B34-164 cultivars (XLSX 18 kb)

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Khueychai, S., Jangpromma, N., Daduang, S. et al. Comparative proteomic analysis of leaves, leaf sheaths, and roots of drought-contrasting sugarcane cultivars in response to drought stress. Acta Physiol Plant 37, 88 (2015). https://doi.org/10.1007/s11738-015-1826-7

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  • DOI: https://doi.org/10.1007/s11738-015-1826-7

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