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Root-applied brassinolide can alleviate the NaCl injuries on cotton

Acta Physiologiae Plantarum volume 37, Article number: 75 (2015) Cite this article

Abstract

Brassinolide (BL) is a plant growth regulator. This study analyzes the effects of BL on cotton growth, Na+ accumulation, proline content, MDA content, antioxidant enzyme activities, and differentially expressed genes (DEGs) of cotton roots under NaCl stress. We grew two cotton cultivars, Sumian 12 (salt sensitive) and Sumian 22 (salt tolerant), in continuously aerated Hoagland’s nutrient solution supplemented with 200 mM NaCl. NaCl stress increased the Na+, proline, and MDA content and decreased root activity and protein content in the roots of Sumian 12 and 22, and the change rang of Sumian 12 was higher than that of Sumian 22. The application of BL counteracted the NaCl stress-induced growth inhibition in the two tested cotton cultivars. It reduced the accumulation of Na+, enhanced proline content, and resulted in an overall change in the activities of antioxidant enzymes causing a decrease in the MDA content of NaCl-stressed roots, and the influence of BL on salt-stressed Sumian 12 plants was more pronounced than that on Sumian 22. The digital gene expression analysis in Sumian 12 indicated that BL application significantly influenced the gene expression in NaCl-stressed roots. The majority of 7659 (3661 up-regulated/3998 down-regulated) DEGs of NaCl/CK in roots of Sumian 12 were regulated by BL, and the gene expression pattern as a result of the root-applied BL on NaCl-stressed cotton treatment (BL + NaCl) was similar to the control. Our results indicate that the root-applied brassinolide alleviates NaCl stress on cotton through improving root activity, physiology, and gene expression.

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Abbreviations

BL:

Brassinolide

BRs:

Brassinosteroids

DEGs:

Differentially expressed genes

MDA:

Malondialdehyde

SOD:

Superoxide dismutase

CAT:

Catalase

POD:

Peroxidase

References

  • Ahammed GJ, Choudhary SP, Chen S, Xia X, Shi K, Zhou Y, Yu J (2013) Role of brassinosteroids in alleviation of phenanthrene-cadmium co-contamination-induced photosynthetic inhibition and oxidative stress in tomato. J Exp Bot 63(1):199–213

    Article  Google Scholar 

  • Ali B, Hasan SA, Hayat S, Hayat Q, Yadav S, Fariduddin Q, Ahmad A (2001) A role for brassinosteroids in the amelioration of aluminium stress through antioxidant system in mung bean (Vigna radiata L. Wilczek). Environ Exp Bot 62:153–159

    Article  Google Scholar 

  • Ali Q, Athar HR, Ashraf M (2008) Modulation of growth, photosynthetic capacity and water relations in salt stressed wheat plants by exogenously applied 24-epibrassinolide. Plant Growth Regul 56(2):107–116

    Article  CAS  Google Scholar 

  • Amalo K, Chen GX, Asade K (1994) Separate assays specific for ascorbate peroxidase and guaiacol peroxidase and for the chloroplastic and cytosolic isozymes of ascorbate peroxidase in plants. Plant Cell Physiol 35:497–504

    Google Scholar 

  • Ashraf M, Ahmad S (2000) Influence of sodium chloride on ion accumulation, yield components and fibre characteristics in salt-tolerant and salt-sensitive lines of cotton (Gossypium hirsutum L.). Field Crops Res 66:115–127

    Article  Google Scholar 

  • Bajguz A (2000) Effect of brassinosteroids on nucleic acid and protein content in cultured cells of Chlorella vulgaris. Plant Physiol Biochem 38:209–215

    Article  CAS  Google Scholar 

  • Bajguz A, Hayat S (2009) Effects of brassinosteroids on the plant responses to environmental stresses. Plant Physiol Biochem 47:1–8

    Article  CAS  PubMed  Google Scholar 

  • Bandeh-hagh A, Toorchi M, Mohammadi A, Chaparzadeh N, Salekdeh GH, Kazemnia H (2008) Growth and osmotic adjustment of canola genotypes in response to salinity. J Food Agric Environ 6(2):201–208

    CAS  Google Scholar 

  • Bates LS, Waldeen RP, Teare ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–207

    Article  CAS  Google Scholar 

  • Chen Z, Pottosin II, Cuin TA, Fuglsang AT, Tester M, Jha D, Zepeda-Jazo I, Zhou M, Palmgren MG, Newman IA, Shabala S (2007) Root plasma membrane transporters controlling K+/Na+ homeostasis in salt stressed barley. Plant Physiol 145:1714–1725

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Clouse SD, Sasse J (1998) Brassinosteroids: essential regulators of plant growth and development. Annu Rev Plant Physiol Plant Mol Biol 49:427–451

    Article  CAS  PubMed  Google Scholar 

  • Dhaubhadel S, Krishna P (2008) Identification of differentially expressed genes in brassinosteroid-treated brassica napus seedlings. J Plant Growth Regul 27:297–300

    Article  CAS  Google Scholar 

  • Divi UK, Krishna P (2009) Brassinosteroid: a biotechnological target for enhancing crop yield and stress tolerance. New Biotechnol 36:131–136

    Article  Google Scholar 

  • Foster JG, Hess JL (1980) Responses of superoxide dismutase and glutathione reductase activities in cotton leaf tissue exposed to an atmosphere enriched in oxygen. Plant Physiol 66:482–487

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Bioch 48:909–930

    Article  CAS  Google Scholar 

  • Goetz M, Godt DE, Roitsch T (2000) Tissue-specific induction of the mRNA for an extracellular invertase isoenzyme of tomato by brassinosteroids suggests a role for steroid hormones in assimilate partitioning. Plant J 22:515–522

    Article  CAS  PubMed  Google Scholar 

  • Hayat S, Maheshwari P, Wani AS, Irfan M, Alyemeni MN, Ahmad A (2012) Comparative effect of 28 homobrassinolide and salicylic acid in the amelioration of NaCl stress in Brassica juncea L. Plant Physiol Bioch 53:61–68

    Article  CAS  Google Scholar 

  • Jaarsma R, Vries RSM, Boer AH (2013) Effect of salt stress on growth, Na+ accumulation and proline metabolism in potato (Solanum tuberosum) cultivars. PLoS One 8(3):e60183. doi:10.1371/journal.pone.0060183

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Kang YY, Guo SR, Li J, Duan JJ (2009) Effect of root applied 24-epibrassinolide on carbohydrate status and fermentative enzyme activities in cucumber (Cucumis sativus L.) seedlings under hypoxia. Plant Growth Regul 57:259–269

    Article  CAS  Google Scholar 

  • Kartal G, Temel A, Arican E, Gozukirmizi N (2009) Effects of brassinosteroids on barley root growth, antioxidant system and cell division. Plant Growth Regul 58:261–267

    Article  CAS  Google Scholar 

  • Li HS (2000) Principles and techniques of plant physiological experiments. Higher Education Press, Beijing, pp 119–167 (in Chinese)

    Google Scholar 

  • Li ZJ, Xie XY, Zhang SQ, Liang YC (2011) Negative effects of oxytetracycline on wheat (Triticum aestivum L.) growth, root activity, photosynthesis, and chlorophyll contents. Agric Sci China 10:1545–1553

    Article  CAS  Google Scholar 

  • Liu Y, Zhao Z, Si J, Di C, Han J, An L (2009) Brassinosteroids alleviate chilling -induced oxidative damage by enhancing antioxidant defense system in suspension cultured cells of Chorispora bungeana. Plant Growth Regul 59:207–214

    Article  CAS  Google Scholar 

  • Mortazavi A, Williams BA, McCue K, Schaeffer L, Wold B (2008) Mapping and quantifying mammalian transcriptomes by RNA-Seq. Nat Methods 5:621–628

    Article  CAS  PubMed  Google Scholar 

  • Neto ADA, Prisco JT, Eneas-Filho J, Abreu CEB, Gomez-Filho E (2006) Effect of salt stress on antioxidative enzymes and lipid peroxidation in leaves and roots of salt-tolerant and salt-sensitive maize genotypes. Environ Exp Bot 56:87–94

    Article  Google Scholar 

  • Oueslati S, Karray-Bouraoui N, Attia H, Rabhi M, Ksouri R, Lachaal M (2010) Physiological and antioxidant responses of Mentha pulegium (Pennyroyal) to salt stress. Acta Physiol Plant 32:289–296

    Article  CAS  Google Scholar 

  • Sairam PK, Srivastava GC (2002) Changes in antioxidant activity in sub-cellular fractions of tolerant and susceptible wheat genotypes in response to long term salt stress. Plant Sci 162:897–904

    Article  CAS  Google Scholar 

  • Shahbaz M, Ashraf M, Athar HR (2008) Does exogenous application of 24-epibrassinolide ameliorate salt induced growth inhibition in wheat (Triticum aestivum L.)? Plant Growth Regul 55:51–64

    Article  CAS  Google Scholar 

  • Sharma I, Bhardwaj R, Pati PK (2012) Mitigation of adverse effects of chlorpyrifos by 24-epibrassinolide and analysis of stress markers in a rice variety Pusa Basmati-1. Ecotox Environ Safe 85:72–81

    Article  CAS  Google Scholar 

  • Sharma I, Ching E, Saini R, Bhardwaj S, Pati PK (2013) Exogenous application of brassinosteroid offers tolerance to salinity by altering stress responses in rice variety Pusa Basmati-1. Plant Physiol Biochem 69:17–26

    Article  CAS  PubMed  Google Scholar 

  • Shu HM, Guo SQ, Shen XL, Ni WC (2011) Effects of brassinosteroid on cotton physiology under NaCl stress. Jiangsu J Agr Sci 27(6):1198–1202 (in Chinese)

    Google Scholar 

  • Symons GM, Reid JB (2004) Brassinosteroids do not undergo long-distance transport in pea. Implications for the regulation of endogenous brassinosteroid levels. Plant Physiol 135:2196–2206

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Wu CY, Trieu A, Radhakrishnan P, Kwok SF, Harris S, Zhang K, Wang J, Wan J, Zhai H, Takatsuto S, Matsumoto S, Fujioka S, Feldmann KA, Pennella RI (2008) Brassinosteroids regulate grain filling in rice. Plant Cell 20:2130–2145

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Yao D, Zhang X, Zhao X, Liu C, Wang C, Zhang Z, Zhang C, Wei Q, Wang Q, Yan H, Li F, Su Z (2011) Transcriptome analysis reveals salt-stress-regulated biological processes and key pathways in roots of cotton (Gossypium hirsutum L.). Genomics 98(1):47–55

    Article  CAS  PubMed  Google Scholar 

  • Zeng H, Tang Q, Hua X (2010) Arabidopsis brassinosteroid mutants det 2-1 and bin 2-1 display altered salt tolerance. J Plant Growth Regul 29:44–52

    Article  Google Scholar 

  • Zhang GW, Lu HL, Zhang L, Chen BL, Zhou ZG (2011a) Salt tolerance evaluation of cotton (Gossypium hirsutum L.) at its germinating and seedling stages and selection of related indices. Chin J Appl Ecol 22:2045–2053 (in Chinese)

    CAS  Google Scholar 

  • Zhang H, Shen G, Kuppu S, Gaxiola R, Payton P (2011b) Creating drought- and salt-tolerant cotton by overexpressing a vacuolar pyrophosphatase gene. Plant Signal Behav 6(6):861–863

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Zhang L, Chen B, Zhang G, Li J, Wang Y, Meng Y, Zhou Z (2013a) Effect of soil salinity, soil drought, and their combined action on the biochemical characteristics of cotton roots. Acta Physiol Plant 35:3167–3179

    Article  CAS  Google Scholar 

  • Zhang L, Zhang G, Wang Y, Zhou Z, Meng Y, Chen B (2013b) Effect of soil salinity on physiological characteristics of functional leaves of cotton plants. J Plant Res 126:293–304

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was funded by the National Natural Science Foundation of China (31201139), National R&D Project of Transgenic Crops of Ministry of Science and Technology of China (2011ZX08005-001), and Jiangsu Agricultural science and technology innovation funds (CX (13) 5011).

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Authors and Affiliations

  1. Key Laboratory of Cotton and Rapeseed (Nanjing), Ministry of Agriculture, Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, People’s Republic of China

    Hongmei Shu, Wanchao Ni, Shuqiao Guo, Yuanyong Gong, Xinlian Shen, Xianggui Zhang, Peng Xu & Qi Guo

Authors
  1. Hongmei Shu
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  2. Wanchao Ni
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  3. Shuqiao Guo
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  4. Yuanyong Gong
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  5. Xinlian Shen
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  6. Xianggui Zhang
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  7. Peng Xu
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  8. Qi Guo
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Corresponding author

Correspondence to Wanchao Ni.

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Communicated by R. Aroca.

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Shu, H., Ni, W., Guo, S. et al. Root-applied brassinolide can alleviate the NaCl injuries on cotton. Acta Physiol Plant 37, 75 (2015). https://doi.org/10.1007/s11738-015-1823-x

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  • DOI: https://doi.org/10.1007/s11738-015-1823-x

Keywords

  • Brassinolide
  • Cotton
  • NaCl stress
  • Growth
  • Root physiology
  • Differentially expressed genes