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Analysis of thylakoid membrane protein and photosynthesis-related key enzymes in super high-yield hybrid rice LYPJ grown in field condition during senescence stage

Acta Physiologiae Plantarum volume 37, Article number: 1 (2015) Cite this article

Abstract

A proteomic analysis combining photosynthesis-related key enzymes measurement and mRNA level analysis was used to investigate the variations of proteins involved in the photosynthetic apparatus in flag leaves of high-yield rice LYPJ during senescence under field conditions. The stability of chlorophyll-protein complexes of thylakoid membranes was measured by BN-PAGE system. Our findings revealed that the LHCII complexes were more stable than F0F1-ATPase and Cyb 6 /f complexes. Thylakoid membranes proteins during the senescence stage were separated by two-dimensional gel electrophoresis. We identified 13 photosynthesis-related proteins (a fivefold or greater variation), including electron transport, Calvin–Benson cycle and chloroplast protease during senescence of flag leaves. Moreover, our data showed that Ca2+-ATPase was more stable during senescence of flag leaves in rice, compared to Mg2+-ATPase. In enzymes activities analyses, it was observed that a little increment of C4 photosynthetic enzymes activities at the beginning of flag leaves senescence might compensate, to some degree, for the larger reductions in Rubisco activity, confirmed by quantitative RT-PCR analysis.

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (No. 31271621), Natural Science Foundation of Jiangsu Province, China (No. 11KJA180001), the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) and NSFC for Talents Training in Basic Science (J1103507, J1210025).

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Authors and Affiliations

  1. Jiangsu Key Laboratory of Biodiversity and Biotechnology, Life Sciences College, Nanjing Normal University, Nanjing, 210023, China

    Yuwen Wang, Jing Yu, Xiaohan Jiang, Lingang Sun, Kang Li, Pingyang Wang, Min Wu & Guoxiang Chen

  2. Zijin College, Nanjing University of Science and Technology, Nanjing, 210023, China

    Min Wu

  3. Institute of Food and Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China

    Chuangen Lv

Authors
  1. Yuwen Wang
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  2. Jing Yu
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  3. Xiaohan Jiang
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  4. Lingang Sun
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  5. Kang Li
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  6. Pingyang Wang
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  7. Min Wu
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  8. Guoxiang Chen
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  9. Chuangen Lv
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Correspondence to Guoxiang Chen.

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Communicated by J. Gao.

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Wang, Y., Yu, J., Jiang, X. et al. Analysis of thylakoid membrane protein and photosynthesis-related key enzymes in super high-yield hybrid rice LYPJ grown in field condition during senescence stage. Acta Physiol Plant 37, 1 (2015). https://doi.org/10.1007/s11738-014-1746-y

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  • DOI: https://doi.org/10.1007/s11738-014-1746-y

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