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Indole acetic acid modulates changes in growth, chlorophyll a fluorescence and antioxidant potential of Trigonella foenum-graecum L. grown under cadmium stress

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Abstract

Indole acetic acid at low (IAAL, 10 µM) and high (IAAH, 100 µM) dose-induced responses on growth, chlorophyll a fluorescence and antioxidant potential of widely cultivated Trigonella foenum-graecum L. seedlings grown under cadmium (Cd1, 3 mg Cd Kg−1 soil and Cd2, 9 mg Cd Kg−1 soil) stress were investigated. Cadmium (Cd) at tested doses reduced the growth, pigment contents, photosynthetic (O2 evolution) and carbonic anhydrase (CA) activity which was accompanied with Cd accumulation in tissues. To quantify the performance of photosystem (PS) II, chlorophyll a fluorescence (JIP test) was analyzed and under Cd stress, the yield for primary photochemistry (φP 0), yield of electron transport per trapped exciton (ψ 0), quantum yield of electron transport (φE 0) and performance index of PS II (PIABS) were decreased, while it induced significant rise in energy flux parameters. Foliar application of IAAL dose causes significant reduction in Cd accumulation and hence alleviated the toxic effects of Cd on these parameters appreciably; while at IAAH dose, Cd induced-effects were further aggravated. Respiratory O2 uptake was increased progressively with rising concentration of Cd, while together with IAA, it showed reverse trend. Cd alone and together with IAAH enhanced the oxidative markers: O2 •−, H2O2 and MDA contents despite of the significant increase in SOD and CAT activity; while with IAAL, these markers were declined significantly. Overall results suggest that application of IAAL reduced the Cd accumulation in tissues significantly; hence, increased activity of photosynthesis and antioxidant potential improved the growth performance of Trigonella seedlings grown under Cd stress.

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Abbreviations

ABS/RC:

The energy fluxes for absorption of photon per active reaction center

CA:

Carbonic anhydrase

CAT:

Catalase

DI0/RC:

Energy dissipation flux per active reaction center

ET0/RC:

Electron transport flux per active reaction center

IAA:

Indole acetic acid

MDA:

Malondialdehyde

Phi_E 0 or φE 0 :

Quantum yield of electron transport

Phi_P 0 or φP 0 :

The quantum yield of primary photochemistry

PIABS :

Performance index of PSII

Psi_0 or Ψ 0 :

Yield of electron transport per trapped exciton

PS II:

Photosystem II

QA:

Primary electron accepter of PS II

RC:

Reaction center

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

SOR:

Superoxide radical

TR0/RC:

Trapped energy flux per active reaction center

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Acknowledgments

The authors gratefully acknowledge the Head, Department of Botany, University of Allahabad, Allahabad, for providing necessary facilities and also to Indian Council of Medical and Research, New Delhi, India for providing financial support to Gausiya Bashri as Junior Research Fellow.

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Authors and Affiliations

  1. Ranjan Plant Physiology and Biochemistry Laboratory, Department of Botany, University of Allahabad (A Central University of India), Allahabad, 211002, India

    Gausiya Bashri & Sheo Mohan Prasad

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  1. Gausiya Bashri
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  2. Sheo Mohan Prasad
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Correspondence to Sheo Mohan Prasad.

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Communicated by H. Li.

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Bashri, G., Prasad, S.M. Indole acetic acid modulates changes in growth, chlorophyll a fluorescence and antioxidant potential of Trigonella foenum-graecum L. grown under cadmium stress. Acta Physiol Plant 37, 49 (2015). https://doi.org/10.1007/s11738-014-1745-z

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  • DOI: https://doi.org/10.1007/s11738-014-1745-z

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