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Detachment-accelerated ripening and senescence of strawberry (Fragaria × ananassa Duch. cv. Akihime) fruit and the regulation role of multiple phytohormones

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To study the detachment stress on the ripeness of strawberry fruit, physiological characteristics of strawberry fruit on and off plant during ripeness and senescence processes were investigated. The results indicated that the ripeness of strawberry fruit upon detachment was accelerated, in terms of firmness, soluble solid content and especially color development. The color of fruit off plant changed rapidly from white to full red in 1–2 days. The respiratory rate in fruit off plant was strengthened, higher than that on plant. Abscisic acid level and ethylene production in fruit off plant were also higher than those on plant and auxin degradation was exacerbated by detachment. Expression levels of FaMYB1, FabHLH3 and FaTTG1 were generally reduced with phenotypes of redder color and more anthocyanin accumulation in fruit off plant. Results also suggested that the detachment initially stimulated ethylene and abscisic acid production and auxin degradation, which modulated ripening-related gene expression and at last enhanced fruit pigmentation.

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  • Abeles FB, Morgan PW, Saltveit JME (1992) Ethylene in plant biology. (Access Online via Elsevier)

  • Aharoni A, De Vos C, Wein M, Sun Z, Greco R, Kroon A, Mol J, O’Connell A (2001) The strawberry FaMYB1 transcription factor suppresses anthocyanin and flavonol accumulation in transgenic tobacco. Plant J 28:319–332

    Article  CAS  PubMed  Google Scholar 

  • Bower J, Holford P, Latché A, Pech JC (2002) Culture conditions and detachment of the fruit influence the effect of ethylene on the climacteric respiration of melon. Postharvest Biol Technol 26:135–146

    Article  CAS  Google Scholar 

  • Bower JH, Blasi WV, Mitcham EJ (2003) Effects of ethylene and 1-MCP on the quality and storage life of strawberries. Postharvest Biol Technol 28:417–423

    Article  CAS  Google Scholar 

  • Brueggemann J, Weisshaar B, Sagasser M (2010) A WD40-repeat gene from Malus × domestica is a functional homologue of Arabidopsis thaliana TRANSPARENT TESTA GLABRA1. Plant Cell Rep 29:285–294

    Article  CAS  PubMed  Google Scholar 

  • Chang S, Puryear J, Cairney J (1993) A simple and efficient method for isolating RNA from pine trees. Plant Mol Biol Rep 11:113–116

    Article  CAS  Google Scholar 

  • Chen F, Liu H, Yang H, Lai S, Cheng X, Xin Y, Yang B, Hou H, Yao Y, Zhang S, Bu G, Deng Y (2011) Quality attributes and cell wall properties of strawberries (Fragaria annanassa Duch) under calcium chloride treatment. Food Chem 126:450–459

    Article  CAS  Google Scholar 

  • Cheng GW, Breen PJ (1991) Activity of phenylalanine ammonia-Lyase (PAL) and concentrations of anthocyanins and phenolics in developing strawberry fruit. J Am Soc Hortic Sci 116:865–869

    CAS  Google Scholar 

  • Field TS, Lee DW, Holbrook NM (2001) Why leaves turn red in autumn the role of anthocyanins in senescing leaves of Red-Osier Dogwood. Plant Physiol 127:566–574

    Article  CAS  Google Scholar 

  • Figueroa CR, Opazo MC, Vera P, Arriagada O, Diaz M, Alejandra Moya-Leon M (2012) Effect of postharvest treatment of calcium and auxin on cell wall composition and expression of cell wall-modifying genes in the Chilean strawberry (Fragaria chiloensis) fruit. Food Chem 132:2014–2022

    Article  CAS  Google Scholar 

  • Fuggate P, Wongs-Aree C, Noichinda S, Kanlayanarat S (2010) Quality and volatile attributes of attached and detached ‘Pluk Mai Lie’ papaya during fruit ripening. Sci Hortic 126:120–129

    Article  CAS  Google Scholar 

  • Given NK, Venis MA, Grierson D (1988) Phenylalanine ammonia-lyase activity and anthocyanin synthesis in ripening strawberry fruit. J Plant Physiol 133:25–30

    Article  CAS  Google Scholar 

  • Hörtensteiner S (2006) Chlorophyll degradation during senescence. Annu Rev Plant Biol 57:55–77

    Article  PubMed  Google Scholar 

  • Iannetta PPM, Laarhoven LJ, Medina-Escobar N, James EK, McManus MT, Davies HV, Harren FJM (2006) Ethylene and carbon dioxide production by developing strawberries show a correlative pattern that is indicative of ripening climacteric fruit. Physiol Plant 127:247–259

    Article  CAS  Google Scholar 

  • Inskeep WP, Bloom PR (1985) Extinction coefficients of chlorophyll-a and chlorophyll-b in N, N-dimethylformamide and 80-percent acetone. Plant Physiol 77:483–485

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Jeong ST, Goto-Yamamoto N, Kobayashi S, Esaka M (2004) Effects of plant hormones and shading on the accumulation of anthocyanins and the expression of anthocyanin biosynthetic genes in grape berry skins. Plant Sci 167:247–252

    Article  CAS  Google Scholar 

  • Jia HF, Chai YM, Li CL, Lu D, Luo JJ, Qin L, Shen YY (2011) Abscisic acid plays an important role in the regulation of strawberry fruit ripening. Plant Physiol 157:188–199

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Jiang Y, Joyce D (2003) ABA effects on ethylene production, PAL activity, anthocyanin and phenolic contents of strawberry fruit. Plant Growth Regul 39:171–174

    Article  Google Scholar 

  • Jiang Y, Joyce DC, Terry LA (2001) 1-Methylcyclopropene treatment affects strawberry fruit decay. Postharvest Biol Technol 23:227–232

    Article  CAS  Google Scholar 

  • Kader AA (1999) Fruit maturity, ripening, and quality relationships. In: Michalezuk L (ed) International symposium on effect of preharvest and postharvest. Factors on storage of fruit, pp 203–208

  • Lin SF, Walsh CS (2008) Studies of the “tree factor” and its role in the maturation and ripening of ‘Gala’ and ‘Fuji’ apples. Postharvest Biol Technol 48:99–106

    Article  Google Scholar 

  • Lin-Wang K, Bolitho K, Grafton K, Kortstee A, Karunairetnam S, McGhie T, Espley R, Hellens R, Allan A (2010) An R2R3 MYB transcription factor associated with regulation of the anthocyanin biosynthetic pathway in Rosaceae. BMC Plant Biol 10:50

    PubMed Central  PubMed  Google Scholar 

  • Lin-Wang K, Micheletti D, Palmer J, Volz R, Lozano L, Espley R, Hellens RP, Chagne D, Rowan DD, Troggio M, Iglesias I, Allan AC (2011) High temperature reduces apple fruit colour via modulation of the anthocyanin regulatory complex. Plant Cell Environ 34:1176–1190

    Article  PubMed  Google Scholar 

  • Lopes-da-Silva F, Escribano-Bailón MT, Pérez-Alonso JJ, Rivas-Gonzalo JC, Santos-Buelga C (2007) Anthocyanin pigments in strawberry. Lebenson Wiss Technol 40:374–382

    Article  CAS  Google Scholar 

  • Lopez AP, Portales RB, López-Ráez JA, Medina-Escobar N, Blanco JM, Franco AR (2006) Characterization of a strawberry late-expressed and fruit-specific peptide methionine sulphoxide reductase. Physiol Plant 126:129–139

    Article  CAS  Google Scholar 

  • Marty I, Douat C, Tichit L, Jungsup K, Leustek T, Abagnac G (2000) The cystathionine-γ-synthase gene involved in methionine biosynthesis is highly expressed and auxin-repressed during wild strawberry (Fragaria vesca L) fruit ripening. Theor Appl Genet 100:1129–1136

    Article  CAS  Google Scholar 

  • Nguyen H, Kim J, Hyun W, Nguyen N, Hong SW, Lee H (2013) TTG1-mediated flavonols biosynthesis alleviates root growth inhibition in response to ABA. Plant Cell Rep 32:503–514

    Article  CAS  PubMed  Google Scholar 

  • Ornelas-Paz, J de J, Yahia EM, Ramírez-Bustamante N, Pérez-Martínez JD, Escalante-Minakata, M de P, Ibarra-Junquera V, Acosta-Muñiz C, Guerrero-Prieto V, Ochoa-Reyes E (2013) Physical attributes and chemical composition of organic strawberry fruit (Fragaria × ananassa Duch, cv. Albion) at six stages of ripening. Food Chem 138:372–381

    Article  CAS  Google Scholar 

  • Paul V, Pandey R, Srivastava G (2012) The fading distinctions between classical patterns of ripening in climacteric and non-climacteric fruit and the ubiquity of ethylene—an overview. J Food Sci Technol 49:1–21

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Rogiers SY, Knowles NR (1999) A comparison of preharvest and postharvest ethylene production and respiration rates of saskatoon (Amelanchier alnifolia Nutt) fruit during development. Can J Bot 77:323–332

    CAS  Google Scholar 

  • Saure MC (1990) External control of anthocyanin formation in apple. Sci Hortic 42:181–218

    Article  CAS  Google Scholar 

  • Schaart JG, Dubos C, De La Fuente IR, van Houwelingen A, de Vos RCH, Jonker HH, Xu WJ, Routaboul JM, Lepiniec L, Bovy AG (2013) Identification and characterization of MYB-bHLH-WD40 regulatory complexes controlling proanthocyanidin biosynthesis in strawberry (Fragaria × ananassa) fruit. New Phytol 197:454–467

    Article  CAS  PubMed  Google Scholar 

  • Shiomi S, Wamocho LS, Agong SG (1996) Ripening characteristics of purple passion fruit on and off the vine. Postharvest Biol Technol 7:161–170

    Article  Google Scholar 

  • Symons GM, Chua YJ, Ross JJ, Quittenden LJ, Davies NW, Reid JB (2012) Hormonal changes during non-climacteric ripening in strawberry. J Exp Bot 63:4741–4750

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Tadesse T, Nichols MA, Hewett EW (1998) Ripening of attached and detached sweet pepper fruit cv. ‘Domino’. In: Bieleski R, Laing WA, Clark CJ (eds) Postharvest ‘96—proceedings of the international postharvest science conference, vol 464, pp 503

  • Tian MS, Prakash S, Elgar HJ, Young H, Burmeister DM, Ross GS (2000) Responses of strawberry fruit to 1-methylcyclopropene (1-MCP) and ethylene. Plant Growth Regul 32:83–90

    Article  CAS  Google Scholar 

  • Trainotti L, Pavanello A, Casadoro G (2005) Different ethylene receptors show an increased expression during the ripening of strawberries: does such an increment imply a role for ethylene in the ripening of these non-climacteric fruit? J Exp Bot 56:2037–2046

    Article  CAS  PubMed  Google Scholar 

  • Villarreal NM, Bustamante CA, Civello PM, Martínez GA (2010) Effect of ethylene and 1-MCP treatments on strawberry fruit ripening. J Sci Food Agric 90:683–689

    CAS  PubMed  Google Scholar 

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This research is supported by the National Basic Research Program (973 program) of China (2013CB127101) and the National Natural Science Foundation of China (31372113).

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The authors declare that they have no conflict of interest.

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Correspondence to Linchun Mao.

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Communicated by P. K. Nagar.

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Chen, J., Mao, L., Mi, H. et al. Detachment-accelerated ripening and senescence of strawberry (Fragaria × ananassa Duch. cv. Akihime) fruit and the regulation role of multiple phytohormones. Acta Physiol Plant 36, 2441–2451 (2014).

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