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24-Epibrassinolide regulates carbohydrate metabolism and increases polyamine content in cucumber exposed to Ca(NO3)2 stress

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Abstract

Key message

This study focuses on the impact of carbohydrate metabolism and endogenous polyamines levels in leaves of cucumber seedlings under salt stress by exogenous BRs.

Abstract

The effects of 24-epibrassinolide (EBL) on carbohydrate metabolism and endogenous content of polyamines were investigated in cucumber seedlings (Cucumis sativus L. cv. Jinyou No. 4) exposed to salinity stress [80 mM Ca(NO3)2]. Spraying of exogenous EBL partially enhanced the enzyme activities of sucrose phosphate synthase, sucrose synthase and acid invertase; thus, raising the level of sucrose, fructose and total soluble sugars. The amylase activity was also increased by EBL, companied by the rising of sucrose level. These results indicated that EBL improved the carbohydrate metabolism of cucumber under Ca(NO3)2 stress. Moreover, EBL raised the levels of soluble conjugated and insoluble bound polyamines while lowered the free polyamines content, particularly putrescine. Our experiment demonstrated that exogenous EBL elevated stability of cellular membrane and positively improve the carbohydrate metabolism in cucumber growing under Ca(NO3)2 stress.

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Abbreviations

AI:

Acid invertase

BRs:

Brassinosteroids

EBL:

24-Epibrassinolide

MSI:

Membrane stability index

PAs:

Polyamines

Put:

Purtescine

Spd:

Spermidine

Spm:

Spermine

SPS:

Sucrose phosphate synthase

SS:

Sucrose synthase

ROS:

Reactive oxygen species

RWC:

Relative water content

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Acknowledgments

This work was funded by National Basic Research Program of China (973 Program, No. 2009CB119000), National Natural Science Foundation of China (No. 31071831; No. 31272209), and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), and supported by the China Earmarked Fund for Modern Agro-industry Technology Research System (CARS-25-C-03).

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Authors and Affiliations

  1. Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China

    Lingyun Yuan, Shuhai Li, Sheng Shu, Jin Sun & Shirong Guo

  2. College of Horticulture, Anhui Agricultural University, Hefei, 230061, China

    Lingyun Yuan & Shidong Zhu

Authors
  1. Lingyun Yuan
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  2. Shidong Zhu
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  3. Shuhai Li
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  4. Sheng Shu
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  5. Jin Sun
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  6. Shirong Guo
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Corresponding author

Correspondence to Shirong Guo.

Additional information

Communicated by A. Krolicka.

L. Yuan and S. Zhu contributed equally to this paper.

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Yuan, L., Zhu, S., Li, S. et al. 24-Epibrassinolide regulates carbohydrate metabolism and increases polyamine content in cucumber exposed to Ca(NO3)2 stress. Acta Physiol Plant 36, 2845–2852 (2014). https://doi.org/10.1007/s11738-014-1612-y

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  • DOI: https://doi.org/10.1007/s11738-014-1612-y

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