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Cultivar specific variations in morphological and biochemical characteristics of mung bean due to foliar spray of ascorbic acid under elevated ozone

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Abstract

Effect of exogenous application of ascorbic acid (Asc) solution was examined on the growth, photosynthetic pigments, biochemical and yield characteristics of mung bean cultivars against ozone (O3). Experiment was performed on six mung bean cultivars in open top chambers under field conditions and Asc was applied as foliar spray prior to the exposure of ambient (AO+) and elevated (EO+) levels of O3. Application of Asc showed increment in growth attributes as compared to plants not provided Asc (AO). However, O3 induced the production of reactive oxygen species led to membrane damage. Reductions were depicted in lipid peroxidation, solute leakage and foliar injury % in AO+ and EO+ as compared to plants not provided with Asc. Photosynthetic pigments and ascorbic acid content along with activities of anti-oxidative enzymes (APX, CAT, GR and SOD) showed increments in AO+ and EO+ with cultivar-specific variations. Cultivars HUM-1 and HUM-2 restored yield with Asc application while less response was observed in other test cultivars. Quality of the seeds was also affected by Asc treatment in the plants exposed to ambient and elevated levels of O3. Therefore, exogenous application of Asc promotes plant’s performance by providing protection against O3 induced oxidative stress and may be used in screening of the mung bean cultivars against O3 phytotoxicity.

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Acknowledgments

Authors are grateful to the Head, Department of Botany and co-ordinator, CAS in Botany, Banaras Hindu University, Varanasi for providing all the laboratory facilities; University Grants Commission, New Delhi, for financial support and Prof. M.N. Singh, Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, B.H.U., Varanasi, for providing the seeds of mung bean cultivars.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Laboratory of Air Pollution and Global Climate Change, Department of Botany, Banaras Hindu University, Varanasi, 221005, India

    Nivedita Chaudhary & S. B. Agrawal

Authors
  1. Nivedita Chaudhary
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  2. S. B. Agrawal
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Correspondence to S. B. Agrawal.

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Communicated by P. K. Nagar.

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Chaudhary, N., Agrawal, S.B. Cultivar specific variations in morphological and biochemical characteristics of mung bean due to foliar spray of ascorbic acid under elevated ozone. Acta Physiol Plant 36, 1793–1803 (2014). https://doi.org/10.1007/s11738-014-1553-5

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  • DOI: https://doi.org/10.1007/s11738-014-1553-5

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