Abstract
Applying the genomic library construction process and colony screening, a novel aroA gene encoding 5-enopyruvylshikimate-3-phosphate synthase from Proteus mirabilis was identified and isolated. Furthermore, the transgenic Arabidopsis with the novel aroA gene was obtained to confirm the potential of the novel aroA gene in developing glyphosate-resistant crops.
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Acknowledgments
The research was supported financially by the Shanghai National Science Foundation (11ZR14324000, 13ZR1460600), the Development Fund of Shanghai Academy of agricultural Sciences (No. 2011-10), National Natural Science Foundation (31071486, 31200076,31300237), Agriculture science technology achievement transformation fund (133919N1300), the Key Project Fund of Shanghai Minhang Science and Technology Committee (2012MH059), the Key Project Fund of the Shanghai Municipal Committee of Agriculture (No. 2011-1-8) and International Scientific and Technological Cooperation (13440701700, 11230705900).
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Communicated by Y. Wang.
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Tian, YS., Jin, XF., Xu, J. et al. Isolation from Proteus mirabilis of a novel class I 5-enopyruvylshikimate-3-phosphate synthase with glyphosate tolerance in transgenic Arabidopsis thaliana . Acta Physiol Plant 36, 549–554 (2014). https://doi.org/10.1007/s11738-013-1414-7
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DOI: https://doi.org/10.1007/s11738-013-1414-7