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Protection effect of nitric oxide on photosynthesis in rice under heat stress

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Abstract

The effect of exogenous applied nitric oxide on photosynthesis under heat stress was investigated in rice seedlings. High temperature resulted in significant reductions of the net photosynthetic rate (P N) due to non-stomatal components. Application of nitric oxide donors, sodium nitroprusside (SNP) or S-nitrosoglutathione (GSNO), dramatically alleviated the decrease of P N induced by high temperature. Chlorophyll fluorescence measurement revealed that high temperature caused significant increase of the initial fluorescence (F o) and non-photochemical quenching (NPQ) whereas remarkable decrease of the maximal fluorescence (F m), the maximal efficiency of PSII photochemistry (F v/F m), the actual PSII efficiency (ΦPSII), and photochemical quenching (q p). In the presence of SNP or GSNO pretreatment, the increase of F o and decrease of F m, F v/F m, ΦPSII and q p were markedly mitigated, but NPQ was further elevated. Moreover, with SNP or GSNO pretreatment, H2O2 accumulation and electrolyte leakage induced by heat treatment were significantly reduced, whereas zeaxanthin content and carotenoid content relative to chlorophyll were elevated. The potassium salt of 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO), a specific NO scavenger, arrested NO donors mediated effects. These results suggest that NO can effectively protect photosynthesis from damage induced by heat stress. The activation effect of NO on photosynthesis may be mediated by acting as ROS scavenging, or/and alleviating oxidative stress via maintaining higher carotenoid content relative to chlorophyll or/and enhancing thermal dissipation of excess energy through keeping higher level of zeaxanthin content under heat stress.

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Abbreviations

PSII:

Photosystem II

RCs:

Reaction centers

OEC:

Oxygen-evolving complex

P N :

Net photosynthetic rate

T r :

Transpiration rate

g s :

Stomatal conductance

C i :

Intercellular CO2 concentration

F o :

Initial fluorescence

NPQ:

Non-photochemical quenching

F m :

The maximal fluorescence

F v/F m :

The maximal efficiency of PSII photochemistry

ΦPSII :

Actual PSII efficiency

q p :

Photochemical quenching

SNP:

Sodium nitroprusside

cPTIO:

2-(4-Carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide

GSNO:

S-nitrosoglutathione

ROS:

Reactive oxygen species

Gln synthase:

Glutamine synthase

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

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Acknowledgments

This work was supported by the Key Course Construction Program of Shanghai Municipal Education Commission: Plant Physiology; and Alliance Plan of Shanghai Promotion Association of Science and Technology Achievements: The design and application of root-control box.

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Correspondence to Lili Song.

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Communicated by M. Garstka.

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Song, L., Yue, L., Zhao, H. et al. Protection effect of nitric oxide on photosynthesis in rice under heat stress. Acta Physiol Plant 35, 3323–3333 (2013). https://doi.org/10.1007/s11738-013-1365-z

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  • DOI: https://doi.org/10.1007/s11738-013-1365-z

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