Skip to main content

Antioxidant, anticancer and hepatoprotective activities of Cotoneaster horizontalis Decne extract as well as α-tocopherol and amygdalin production from in vitro culture


The phytochemical analysis of the ethanolic extract of branches of Cotoneaster horizontalis, Decne revealed the presence of: β-carotene, ascorbic acid and less amounts of α-tocopherol and amygdalin (vitamin B17) in proportions of: 2,500, 70, 0.093, 0.334 mg 100 g−1, respectively. Acute oral toxicity test revealed its safety profile. In vitro study revealed its good 2, 2-diphenyl-1-picrylhydrazyl radical scavenging and anticancer activities. Invivo study, simultaneous administration of this extract at a dose of 100 or 200 mg kg−1 body weight for 4 weeks, exhibited a significant protection in a dose-dependant manner against hepatotoxicity induced by repeated dose of acetaminophen (1 g kg−1 body weight day−1, p.o.) by preserving the liver function parameters, hepatic redox state and serum lipid profile near the healthy levels. Consequently, in vitro culture was carried out on full or half strength of Murashige and Skoog medium supplemented with different concentrations of benzyl amino purine or kinetin provided shootlets production; different concentrations of 2,4-dichlorophenoxy acetic acid and naphthalene acetic acid showed an increase of callus. Determination of α-tocopherol and amygdalin in different shootlets and callus extracts showed a pronounced increases up to 30.62 and 3.69 mg 100 g−1 in shootlet extract, respectively as well as 26.61 and 12.71 mg 100 g−1 in callus extract, respectively, as compared with those of the mother plant (0.76 and 0.11 mg 100 g−1 extract, respectively).

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2
Fig. 3


  • Bhaumik A, Sharma MC (2002) Therapeutic efficacy of Liv.52 in paracetamol induced hepatopathy in rabbits. Indian J Vet Res 1:8–17

    Google Scholar 

  • Brand-Williams W, Cuvelier ME, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. Lebensm Wiss Technol 28:25–30

    Article  CAS  Google Scholar 

  • Brent J (2005) Critical care toxicology: diagnosis and management of critically poisoned patient. Elsevier Health Sciences,

  • Cakilcioglu U, Turkoglu I (2010) An ethnobotanical survey of medicinal plants in Sivrice (Elazıg-Turkey). J Ethnopharmacol 132:165–175

    PubMed  Article  Google Scholar 

  • Chin JH, Hussin AH, Ismail S (2009) Anti-hepatotoxicity effect of Orthosiphon stamineus Benth against acetaminophen-induced liver injury in rats by enhancing hepatic GST activity. Phcog Res 1:53–58

    Google Scholar 

  • Davern TJ, James LP, Hinson JA, Polson J, Larson AM, Fontana RJ, Lalani E, Munoz S, Shakil AO, Lee WM (2006) Measurement of serum acetaminophen-protein adducts in patients with acute liver failure. Gastroenterology 130:687–694

    PubMed  Article  CAS  Google Scholar 

  • Du Q, Jerz G, Ha Y, Li L, Xu Y, Zhang Q, Zheng Q, Winterhalter P, Ito Y (2005) Semi-industrial isolation of salicin and amygdalin from plant extracts using slow rotary counter-current chromatography. J Chromatogr A 1074:43–46

    PubMed  Article  CAS  Google Scholar 

  • El-Mousallamy AM, Hussein SA, Irmgard M, Nawwar MA (2000) Unusual phenolic glycosides from Cotoneaster orbicularis. Phytochemistry 53:699–704

    PubMed  Article  CAS  Google Scholar 

  • Escriva A, Esteve MJ (2002) Determination of liposoluble vitamins in cooked meals, milk and milk products by liquid chromatography. J Chromatogr A 947:313–318

    PubMed  Article  CAS  Google Scholar 

  • Friedewald WT, Levy RI, Fredrickson DS (1972) Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 18:499–505

    PubMed  CAS  Google Scholar 

  • Kapche GD, Harmut L, Serge F, Simeon FK, Pascal W, Bonaventure TN, Berhanu MA (2007) Lanneanol: a new cytotoxic dihydroalkylcyclohexenol and phenolic compounds from Lannea nigritana (Sc. Ell.) Keay. Indian J Vet Res 35:539–543

    CAS  Google Scholar 

  • Khalifa SF, Loutify MH (2006) Ornamental cultured plant collection. In: The occasion of the first international conference on: strategy of Botanic Gardens, vol 31

  • Konings EJM, Roomans HHS (1996) Evaluation and validation of an LC method for the analysis of carotenoids in vegetables and fruits. J Food Chem 59:599–603

    Article  Google Scholar 

  • Kulkarni KS, Rafiq M, Gopumadhavan S, Venkataranganna MV, Madhumathi BG, Mitra SK (2002) Protective effect of Liv. 52 on Na+-K+-ATPase activity in paracetamol-induced hepatotoxicity. Med Update 5:53–56

    Google Scholar 

  • Mohit D, Parminder N, Jaspreet N, Manish M (2011) Hepatotoxicity V/S hepatoprotective agents-a pharmacological review. Int Res J Pharm 2(3):31–37

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Neilsen SS (1998) Food analysis, 2nd edn. Aspen Publishers, Inc, Gaitherburg, pp 287–288

  • OECD (1996) Guidelines for the testing of chemicals. Test no. 423: acute oral toxicity-acute toxic class method. Organisation for economic co-operation and development, Paris

  • Palme E, Bilia AR, Morelli I (1996) Flavonols and isoflavones from Cotoneaster simonsii. Phytochemistry 42(3):903–905

    Article  CAS  Google Scholar 

  • Raghavendran HR, Sathivel A, Devaki T (2005) Effect of Sargassum polycystum (Phaeophyceae)-sulphated polysaccharide extract against acetaminophen-induced hyperlipidemia during toxic hepatitis in experimental rats. Mol Cell Biochem 276:89–96

    PubMed  Article  CAS  Google Scholar 

  • Rajneesh CP, Manimaran A, Sasikala K R, Adaikappan P (2008) Lipid peroxidation and antioxidant status in patients with breast cancer. Singapore Med J 49(8): 640–463

    Google Scholar 

  • Skehan P, Storeng R (1990) New coloremtric cytotoxicity assay for anti-cancer drug screening. J Natl Cancer Inst 82:1107–1112

    PubMed  Article  CAS  Google Scholar 

  • Wang B, Ji C (2008) Tannin concentration enhances seed caching by scatter-hoarding rodents: an experiment using artificial seeds. Acta Oecol 34:379–385

    Article  Google Scholar 

  • Welcome to

Download references


The authors are thankful to the operating team in laboratory of “in vitro culture” in National Cancer Institute (NCI), Cairo, Egypt for their kind help in running the anticancer activity.

Conflict of interest

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations


Corresponding author

Correspondence to Nadia Sokkar.

Additional information

Communicated by K.-Y. Paek.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Sokkar, N., El-Gindi, O., Sayed, S. et al. Antioxidant, anticancer and hepatoprotective activities of Cotoneaster horizontalis Decne extract as well as α-tocopherol and amygdalin production from in vitro culture. Acta Physiol Plant 35, 2421–2428 (2013).

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI:


  • Cotoneaster horizontalis
  • Bioactive constituents
  • Tissue culture