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Antioxidant, anticancer and hepatoprotective activities of Cotoneaster horizontalis Decne extract as well as α-tocopherol and amygdalin production from in vitro culture

Acta Physiologiae Plantarum volume 35, pages 2421–2428 (2013)Cite this article

Abstract

The phytochemical analysis of the ethanolic extract of branches of Cotoneaster horizontalis, Decne revealed the presence of: β-carotene, ascorbic acid and less amounts of α-tocopherol and amygdalin (vitamin B17) in proportions of: 2,500, 70, 0.093, 0.334 mg 100 g−1, respectively. Acute oral toxicity test revealed its safety profile. In vitro study revealed its good 2, 2-diphenyl-1-picrylhydrazyl radical scavenging and anticancer activities. Invivo study, simultaneous administration of this extract at a dose of 100 or 200 mg kg−1 body weight for 4 weeks, exhibited a significant protection in a dose-dependant manner against hepatotoxicity induced by repeated dose of acetaminophen (1 g kg−1 body weight day−1, p.o.) by preserving the liver function parameters, hepatic redox state and serum lipid profile near the healthy levels. Consequently, in vitro culture was carried out on full or half strength of Murashige and Skoog medium supplemented with different concentrations of benzyl amino purine or kinetin provided shootlets production; different concentrations of 2,4-dichlorophenoxy acetic acid and naphthalene acetic acid showed an increase of callus. Determination of α-tocopherol and amygdalin in different shootlets and callus extracts showed a pronounced increases up to 30.62 and 3.69 mg 100 g−1 in shootlet extract, respectively as well as 26.61 and 12.71 mg 100 g−1 in callus extract, respectively, as compared with those of the mother plant (0.76 and 0.11 mg 100 g−1 extract, respectively).

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Acknowledgments

The authors are thankful to the operating team in laboratory of “in vitro culture” in National Cancer Institute (NCI), Cairo, Egypt for their kind help in running the anticancer activity.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Natural Products and Alternative Medicine Department, Faculty of Pharmacy, King Abdulaziz University, Jeddah, 80200, Kingdom of Saudi Arabia

    Nadia Sokkar

  2. Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Cairo, 11562, Egypt

    Nadia Sokkar

  3. Pharmacognosy Department, Faculty of Pharmacy, Russian Univ. Cairo, Cairo, 11829, Egypt

    Omyma El-Gindi

  4. Ornamental Department, Horticulture Research Institute, Agricultural Research Center, Giza, 12619, Egypt

    Sawsan Sayed

  5. Pharmacognosy Department, Faculty of Pharmacy, AL-Azhr Univ, Cairo, 11884, Egypt

    Shaza Mohamed

  6. Biochemistry Department, National Organization for Drug Control and Research (NODCAR), Giza, 354, Egypt

    Zeinab Ali

  7. Medical Administration, Al-Azhar Univ, Cairo, 11884, Egypt

    Iman Alfishawy

Authors
  1. Nadia Sokkar
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  2. Omyma El-Gindi
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  3. Sawsan Sayed
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  4. Shaza Mohamed
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  5. Zeinab Ali
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Correspondence to Nadia Sokkar.

Additional information

Communicated by K.-Y. Paek.

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Sokkar, N., El-Gindi, O., Sayed, S. et al. Antioxidant, anticancer and hepatoprotective activities of Cotoneaster horizontalis Decne extract as well as α-tocopherol and amygdalin production from in vitro culture. Acta Physiol Plant 35, 2421–2428 (2013). https://doi.org/10.1007/s11738-013-1276-z

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  • DOI: https://doi.org/10.1007/s11738-013-1276-z

Keywords

  • Cotoneaster horizontalis
  • Bioactive constituents
  • Tissue culture