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The zinc finger protein ZAT11 modulates paraquat-induced programmed cell death in Arabidopsis thaliana

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Abstract

Plants use programmed cell death (PCD) as a tool in their growth and development. PCD is also involved in defense against different kinds of stresses including pathogen attack. In both types of PCD, reactive oxygen species (ROS) play an important role. ROS is not only a toxic by-product but also acts as signaling molecule that can trigger defense mechanisms in plant. Earlier transcriptome studies indicated the activation of ROS responsive and/or generating genes. Eight genes were selected with a potential role in ROS-induced PCD pathway and one of them, encoding the zinc finger protein ZAT11 (Zinc Arabidopsis thaliana11) exhibited an altered cell death phenotype. Two independent zat11 mutants in a loh2 (LAG one homologue2) genetic background showed enhanced tolerance to paraquat-induced oxidative stress and PCD; whereas, these double mutants exhibited cell death triggered by Alternaria alternata f. sp. lycopersici-toxin (AAL-toxin) or 3-aminotriazole (AT). This indicates that ZAT11 is involved in an intricate oxidative stress-induced PCD network, and the final outcome depends on ZAT11 interactions with other players specific for the particular types of oxidative stress.

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Acknowledgments

Authors acknowledge Veselin Petrov for critically reading the manuscript. MK Qureshi wishes to thank the Higher Education Commission (HEC) of Pakistan for financial support of part of this project. Part of this project was also financially supported by EC FP7 (project BioSupport, 245588).

Conflict of interest

The authors declare no conflict of interest.

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Authors and Affiliations

  1. Department Molecular Biology of Plants, Groningen Biomolecular Sciences and Biotechnology Institute (GBB), University of Groningen, Linnaeusborg, 9747 AG, Groningen, The Netherlands

    Muhammad Kamran Qureshi, Neerakkal Sujeeth & Jacques Hille

  2. Department of Plant Physiology and Plant Molecular Biology, University of Plovdiv, 24 Tsar Assen str, Plovdiv, 4000, Bulgaria

    Neerakkal Sujeeth & Tsanko S. Gechev

  3. Institute of Molecular Biology and Biotechnologies, 105 Ruski Blvd, Plovdiv, 4000, Bulgaria

    Tsanko S. Gechev

Authors
  1. Muhammad Kamran Qureshi
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  2. Neerakkal Sujeeth
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  3. Tsanko S. Gechev
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  4. Jacques Hille
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Corresponding author

Correspondence to Muhammad Kamran Qureshi.

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Communicated by J.-H. Liu.

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Qureshi, M.K., Sujeeth, N., Gechev, T.S. et al. The zinc finger protein ZAT11 modulates paraquat-induced programmed cell death in Arabidopsis thaliana . Acta Physiol Plant 35, 1863–1871 (2013). https://doi.org/10.1007/s11738-013-1224-y

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  • DOI: https://doi.org/10.1007/s11738-013-1224-y

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