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Oxidative stress in the root nodules of Phaseolus vulgaris is induced under conditions of phosphorus deficiency

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Abstract

One of the most adverse effects of phosphorus (P) deficiency on N2-fixing legumes is the generation of harmful active oxygen species which cause oxidative stress. And although oxidative stress has been widely studied in roots and shoots of various plant species, it has not yet sufficiently been documented in bean nodules so far. In this study, two recombinant inbred lines RIL115 (P-deficiency tolerant) and RIL147 (P-deficiency sensitive) of common bean and Concesa (local variety) were inoculated separately with the reference strain R. tropici CIAT899, RhM11 (R. gallicum) or RhM14 (R. tropici); two local strains of the Marrakesh region of Morocco. Nodulated plants were grown under semi-hydroponic conditions with sufficient or deficient P supply and analyzed for their oxidative responses at the flowering stage. The results indicated that P-deficiency decreased the growth of shoots (48 %) and nodules (32 %), particularly with RhM14 exhibiting the highest decrease (52 %) of nodulation. This constraint increased electrolyte leakage of nodules (40 %) as compared to leaves (20 %), especially for plants inoculated with RhM14 and CIAT899. Moreover, high H2O2 and malondialdehyde contents were noticed in P-deficient nodules of RhM14 and RhM11. These variations were associated with peroxidase activity stimulation in P-deficient nodules induced by CIAT899 and RhM14. In symbiosis with RIL115, these last strains exhibited the highest nodule phenol content. Overall, phenol content was mainly enhanced in P-deficient nodules (35 %) as compared to the leaves (16 %). It was concluded that the genotypes inoculated with CIAT899 and RhM11 are relatively P-deficiency tolerant combinations as compared to those inoculated with RhM14. Increase of oxidative stress in nodules rather than in leaves points to the need for further investigations of mechanisms that improve the root-nodule efficiency under adverse conditions.

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Acknowledgments

This work was supported by a ministerial fellowship (CNRST of Morocco) and also by AVERROES scholarship program provided by European Union. We thank Mr Brahim Ait HADDOU and Dr. Usman IRSHAD for editing the manuscript in English.

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Authors and Affiliations

  1. Faculté des Sciences et Techniques Guéliz, Equipe de Biotechnologie Végétale et Agrophysiologie des Symbioses, BP 549, 40000, Marrakech, Morocco

    Adnane Bargaz, Mustapha Faghire, Mohamed Farissi & Cherki Ghoulam

  2. INRA, UMR Eco&Sols-Ecologie Fonctionnelle & Biogéochimie Des Sols & Agroécosystèmes, 2 Place Viala, 34060, Montpellier, France

    Adnane Bargaz & Jean-Jacques Drevon

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  1. Adnane Bargaz
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  2. Mustapha Faghire
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  3. Mohamed Farissi
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  4. Jean-Jacques Drevon
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  5. Cherki Ghoulam
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Correspondence to Adnane Bargaz or Cherki Ghoulam.

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Communicated by G. Bartosz.

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Bargaz, A., Faghire, M., Farissi, M. et al. Oxidative stress in the root nodules of Phaseolus vulgaris is induced under conditions of phosphorus deficiency. Acta Physiol Plant 35, 1633–1644 (2013). https://doi.org/10.1007/s11738-012-1206-5

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  • DOI: https://doi.org/10.1007/s11738-012-1206-5

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