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Effect of bioreactor angle and aeration rate on growth and hydromechanics parameters in bioreactor culture of ginseng suspension cells

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Abstract

Suspension cells of Panax ginseng C.A. Meyer were cultivated in 3-L balloon-type bubble bioreactors and the bioreactor with the angle of 90° at the bottom side was optimized. The gaseous composition in plant cell and tissue cultures is regarded as an important factor affecting the plant growth. Gas hold-up was remarkably higher in the bioreactor with an angle of 90° than the other ones. Aeration rates impacted on the growth ratio, the specific O2 uptake rate (SOUR) of ginseng cells were investigated. 0.4 vvm was selected as the optimal aeration rate with a dry weight of 6.45 g L−1. The specific O2 uptake rate in the culture time was detected and reached the top value at the maximum growth ratio.

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Abbreviations

2,4-D:

2,4-Dichlorophenoxyacetic acid

KT:

Kinetin

MS:

Murashige and Skoog (1962)

BTBB:

Balloon-type bubble bioreactor

vvm:

Volumes of gas per culture medium

KLa:

Volumetric oxygen transfer coefficient, Eq. 1 (h−1)

SOUR:

Specific oxygen uptake ratio

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Authors and Affiliations

  1. School of Pharmaceutical Science and Technology, Tianjin University, No. 92 Weijin Road, Nankai District, Tianjin, 300072, China

    Wen-Yuan Gao & Juan Wang

  2. Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China

    Yanyan Dong

  3. Key Laboratory of Industrial Fermentation Microbiology, Tianjin University of Science and Technology, Ministry of Education, Tianjin, 300457, China

    Shuli Man & Beimei Zuo

  4. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China

    Luqi Huang

  5. Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, 100193, China

    Peigen Xiao

Authors
  1. Yanyan Dong
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  2. Wen-Yuan Gao
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  3. Shuli Man
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  4. Beimei Zuo
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  5. Juan Wang
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  6. Luqi Huang
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  7. Peigen Xiao
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Corresponding author

Correspondence to Wen-Yuan Gao.

Additional information

Communicated by K.-Y. Paek.

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Dong, Y., Gao, WY., Man, S. et al. Effect of bioreactor angle and aeration rate on growth and hydromechanics parameters in bioreactor culture of ginseng suspension cells. Acta Physiol Plant 35, 1497–1501 (2013). https://doi.org/10.1007/s11738-012-1190-9

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  • DOI: https://doi.org/10.1007/s11738-012-1190-9

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