Skip to main content
SpringerLink
Log in

Effect of zinc nutrition on salinity-induced oxidative damages in wheat genotypes differing in zinc deficiency tolerance

  • Original Paper
  • Published:
Acta Physiologiae Plantarum Aims and scope Submit manuscript

Abstract

Zinc deficiency and salinity are well-documented soil problems and often occur simultaneously in cultivated soils. Usually, plants respond to environmental stress factors by activating their antioxidative defense mechanisms. The antioxidative response of wheat genotypes to salinity in relation to Zn nutrition is not well understood. So, we investigated the effect of Zn nutrition on the growth, membrane permeability and sulfhydryl group (–SH groups) content of root cells and antioxidative defense mechanisms of wheat plants exposed to salt stress. In a hydroponic experiment, three bread wheat genotypes (Triticum aestivum L. cvs. Rushan, Kavir, and Cross) with different Zn-deficiency tolerance were exposed to adequate (1 μM Zn) and deficient (no Zn) Zn supply and three salinity levels (0, 60, and 120 mM NaCl). The results obtained showed that adequate Zn nutrition counteracted the detrimental effect of 60 mM NaCl level on the growth of all three wheat genotypes while it had no effect on the root and shoot growth of ‘Rushan’ and ‘Kavir’ at the 120 mM NaCl treatment. At the 0 and 60 mM NaCl treatments, Zn application decreased root membrane permeability while increased –SH group content and root activity of catalase (CAT) and superoxide dismutase (SOD) in ‘Rushan’ and ‘Kavir’. In contrast, Zn had no effect on the root membrane permeability and –SH group content of ‘Rushan’ and ‘Kavir’ exposed to the 120 mM NaCl treatment. At all salinity levels, ‘Cross’ plants supplied with Zn had lower root membrane permeability and higher –SH group content compared to those grown under Zn-deficient conditions. At the 0 and 60 salinity levels, Zn-deficient roots of Kavir and Rushan genotype leaked significantly higher amounts of Fe and K than the Zn-sufficient roots. In contrast, at the 120 mM treatment, Zn application had no effect or slightly increased Fe and K concentration in the root ion leakage of these wheat genotypes. For ‘Cross’, at all salinity levels, Zn-deficient roots leaked significantly higher amounts of Fe and K compared with the Zn-sufficient roots. The differential tolerance to salt stress among wheat genotypes examined in this study was related to their tolerance to Zn-deficiency, –SH group content, and root activity of CAT and SOD. Greater tolerance to salinity of Zn-deficiency tolerant genotype ‘Cross’ is probably associated with its greater antioxidative defense capacity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

Abbreviations

CAT:

Catalase

SOD:

Superoxide dismutase

H2O2 :

Hydrogen peroxide

SH:

Sulfhydryl

References

  • Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126

    Article  PubMed  CAS  Google Scholar 

  • Arbona V, Flors V, Garcia-Agustin P, Gomez-Cadenas A (2003) Enzymatic and non-enzymatic antioxidant responses of Carrizo citrange, salt-sensitive citrus rootstock, to different levels of salinity. Plant Cell Physiol 44:388–394

    Article  PubMed  CAS  Google Scholar 

  • Asada K (1994) Production and action of active oxygen species in photosynthetic tissues. In: Foyer CH, Mullineauxs PM (eds) Causes of photooxidative stress and amelioration of defense system in plants. CRC Press, Boca Raton, pp 77–103

    Google Scholar 

  • Bartosz G (1997) Oxidative stress in plants. Acta Physiol Plant 19:47–64

    Article  CAS  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal Biochem 72:248–254

    Article  PubMed  CAS  Google Scholar 

  • Cakmak I (2000) Possible roles of zinc in protecting plant cells from damage by reactive oxygen species. New Phytol 146:185–205

    Article  CAS  Google Scholar 

  • Cakmak I, Engels C (1999) Role of mineral nutrients in photosynthesis and yield formation. In: Rengel Z (ed) Mineral nutrition of crops. Haworth Press, New York, pp 141–168

    Google Scholar 

  • Cakmak I, Atli M, Kaya R, Evliya H, Marschner H (1995) Association of high light and zinc deficiency in cold induced leaf chlorosis in grapefruit and mandarin trees. J Plant Physiol 146:355–360

    Article  CAS  Google Scholar 

  • Cakmak I, Yilmaz A, Kalayci M, Ekiz H, Torun B, Erenoglu B, Braun HJ (1996) Zinc deficiency as a critical problem in wheat production in central Anatolia. Plant Soil 180:165–172

    Article  CAS  Google Scholar 

  • Chen LM, Lin CC, Kao CH (2000) Copper toxicity in the rice seedlings: changes in antioxidative enzyme activities, H2O2 level, and cell wall peroxidase activity in roots. Bot Bull Acad Sin 41:99–103

    CAS  Google Scholar 

  • Dionisio-Sese ML, Tobita S (1998) Antioxidant responses of rice seedlings to salinity stress. Plant Sci 135:1–9

    Article  CAS  Google Scholar 

  • Fendina IS, Tsonev TD, Guleva EI (1994) ABA as a modulator of the response of Pisum sativum to salt stress. J Plant Physiol 143:245–249

    Article  Google Scholar 

  • Gossett DR, Millhollon EP, Lucas MC (1994) Antioxidant responses to NaCl stress in salt-tolerant and salt-sensitive cultivars of cotton. Crop Sci 34:706–714

    Article  CAS  Google Scholar 

  • Hall JL (2002) Cellular mechanisms for heavy metal detoxification and tolerance. J Exp Bot 53:1–11

    Article  PubMed  CAS  Google Scholar 

  • Harris MJ, Outlaw WH (1991) Rapid adjustment of guard-cell abscisic acid levels to current leaf-water status. Plant Physiol 95:171–173

    Article  PubMed  CAS  Google Scholar 

  • Imlay JA (2003) Pathway of oxidative damage. Annu Rev Microbiol 57:395–418

    Article  PubMed  CAS  Google Scholar 

  • Khan MH, Panda SK (2002) Induction of oxidative stress in roots of Oryza sativa L. in response to salt stress. Biol Plant 45:625–627

    Article  CAS  Google Scholar 

  • Khan MA, Ungar IA, Showalter AM (2000) Effects of salinity on growth, water relations and ion accumulation in the subtropical perennial halophyte, Atriplex griffithii var. stocksii. Ann Bot 85:225–232

    Article  CAS  Google Scholar 

  • Khoshgoftarmanesh AH, Shariatmadari H, Karimian N (2006a) Responses of wheat genotypes to zinc fertilization under saline soil conditions. J Plant Nutr 29:1543–1556

    Article  Google Scholar 

  • Khoshgoftarmanesh AH, Shariatmadari H, Karimian N, van der Zee SEATM (2006b) Cadmium and zinc in saline soil solutions and their concentrations in wheat. Soil Sci Soc Am J 70:582–589

    Article  CAS  Google Scholar 

  • Koyro HW (2006) Effect of salinity on growth, photosynthesis, water relations and solute composition of the potential cash crop halophyte Plantago coronopus (L.). Environ Exp Bot 56:136–146

    Article  CAS  Google Scholar 

  • Locy RD, Chang CC, Neilson BL, Singh NK (1996) Photosynthesis in salt-adapted heterotrophic tobacco cells and regenerated plants. Plant Physiol 110:321–328

    PubMed  CAS  Google Scholar 

  • Marschner H (1995) Mineral nutrition of higher plants. Academic Press, London

    Google Scholar 

  • Munns R (2002) Comparative physiology of salt and water stress. Plant Cell Environ 25:239–250

    Article  PubMed  CAS  Google Scholar 

  • Neill S, Desikan R, Hancock J (2002) Hydrogen peroxide signaling. Curr Opin Plant Biol 5:388–395

    Article  PubMed  CAS  Google Scholar 

  • Nishikimi M, Rao NA, Yagi K (1972) The occurrence of superoxide anion in the reaction of reduced phenazine methosulphate and molecular oxygen. Biochem Biophys Res Commun 48:849–854

    Article  Google Scholar 

  • Osmond CB, Grace SC (1995) Perspectives on photoinhibition and photorespiration in the field: quintessential inefficiencies of the light and dark reactions of photosynthesis? J Exp Bot 46:1351–1362

    Article  CAS  Google Scholar 

  • Parida AK, Das AB (2005) Salt tolerance and salinity effects on plants: review. Ecotox Environ Safe 60:324–349

    Article  CAS  Google Scholar 

  • Pinton R, Cakmak I, Marschner H (1994) Zinc deficiency enhanced NAD(P)H-dependent superoxide radical production in plasma membrane vesicles isolated from roots of bean plants. J Exp Bot 45:45–50

    Article  CAS  Google Scholar 

  • Rengel Z (1995) Sulfhydryl groups in root-cell plasma membranes of wheat genotypes differing in zinc efficiency. Physiol Plant 95:604–612

    Article  CAS  Google Scholar 

  • Rout NP, Shaw BP (2001) Salt tolerance in aquatic macrophytes: possible involvement of the antioxidative enzymes. Plant Sci 160:415–423

    Article  PubMed  CAS  Google Scholar 

  • Takkar PN, Walker CD (1993) The distribution and correction of zinc deficiency. In: Robson AD (ed) Zinc in soils and plants. Kluwer Academic Publishers, Dordrecht, pp 151–166

    Chapter  Google Scholar 

  • Tinker PB, Lauchli A (1984) Advances in plant nutrition. Academic Publishers, San Diego

    Google Scholar 

  • USEPA (1995) Method 3051: Microwave assisted acid digestion of sediments, sludges, soils, and oils. Available online at http://www.epa.gov/SW-846/pdfs/3051.pdf (verified 22 July 2004). USEPA, Washington, DC

  • Welch RM, Norvell WA (1993) Growth and nutrient uptake by barley (Hordeum vulgare L. cv. Herta): studies using an N-(2- hydroxyethyl) ethylene dinitrilotriaacetic acid-buffered nutrient solution technique. II. Role of zinc in the uptake and root leakage of mineral nutrients. Plant Physiol 101:627–631

    PubMed  CAS  Google Scholar 

  • Welch RM, Webb MJ, Loneragan JF (1982) Zinc in membrane function and its role in phosphorus toxicity. In: Scaife A (ed), Plant nutrition. Proceedings of the ninth international plant nutrition colloquium, Warwick University, England, August 22–27, Commonwealth Agricultural Bureaux, Farnham House, Farnham Royal, pp 710–715

  • Welch RM, Allaway WH, House WA, Kubota J (1991) Geographic distribution of trace element problems. In: Mortdvedt JJ, Cox FR, Shuman LM, Welch RM (eds) Micronutrients in agriculture. SSSA No. 4. Madison, pp 31–57

  • White JG, Zasoski RH (1999) Mapping soil micronutrients. Field Crops Res 60:11–26

    Article  Google Scholar 

  • Yan B, Dai Q, Liu X, Huang S, Wang Z (1996) Flooding-induced membrane damage, lipid oxidation and activated oxygen generation in corn leaves. Plant Soil 179:261–268

    Article  CAS  Google Scholar 

  • Yeo AR, Flowers TJ (1983) Varietal differences in the toxicity of sodium ions in rice leaves. Physiol Plant 59:189–195

    Article  CAS  Google Scholar 

  • Zhu JK (2001) Plant salt tolerance. Trends Plant Sci 6:66–71

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Soil Sciences, College of Agriculture, Isfahan University of Technology, 84156-83111, Isfahan, Iran

    Bahareh Daneshbakhsh, Amir Hossein Khoshgoftarmanesh & Hossein Shariatmadari

  2. Faculty of Engineering and Natural Sciences, Sabanci University, 34956, Istanbul, Turkey

    Ismail Cakmak

Authors
  1. Bahareh Daneshbakhsh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Amir Hossein Khoshgoftarmanesh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hossein Shariatmadari
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ismail Cakmak
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Amir Hossein Khoshgoftarmanesh.

Additional information

Communicated by G. Bartosz.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Daneshbakhsh, B., Khoshgoftarmanesh, A.H., Shariatmadari, H. et al. Effect of zinc nutrition on salinity-induced oxidative damages in wheat genotypes differing in zinc deficiency tolerance. Acta Physiol Plant 35, 881–889 (2013). https://doi.org/10.1007/s11738-012-1131-7

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11738-012-1131-7

Keywords

Search

Navigation