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Acta Physiologiae Plantarum

, Volume 35, Issue 3, pp 791–800 | Cite as

Change in total phenolic content and antibacterial activity in regenerants of Vitex negundo L.

  • Naseem AhmadEmail author
  • Md Imran Khan
  • Sarfaraz Ahmed
  • Saad Bin Javed
  • Mohammad Faisal
  • Mohammad Anis
  • Sumbul Rehman
  • Syed Mohammad Umair
Original Paper

Abstract

A valuable medicinal plant, Vitex negundo L. has been investigated for its regeneration potential using shoot tip explants. Out of a range of concentrations of cytokinins [6-benzyl adenine (BA), 6-furfurylaminopurine, 2-isopentenyl adenine] used as supplement to Murashige and Skoog medium (MS), BA at 5.0 μM concentration proved best for multiple shoot induction yielding 3.60 ± 0.50 shoots after 8 weeks of culture. Inclusion of a low concentration of an auxin with optimal cytokinin concentration favoured shoot multiplication and the optimum response was observed on MS medium supplemented with BA (5.0 μM) along with α Naphthalene acetic acid (0.5 μM), where 65.0 ± 1.73 % cultures responded with a mean number of 4.80 ± 0.58 shoots per explants after 8 weeks of culture. Ex vitro rooting of in vitro derived microshoots was achieved upon dipping the cut ends of microshoots in 500 μM indole-3-butyric acid for 10 min followed by transfer to thermocol cups containing sterile soilrite. About 95 % of the plantlets survived the acclimatization procedure and were transferred to greenhouse and finally to field. Screening of the antibacterial activity and estimation of total phenolic content of ethanolic extracts of micropropagated plants were also carried out and compared with that of the mother plant.

Keywords

Vitex negundo L. Regeneration Acclimatization Antimicrobial activity Total phenolic content 

Abbreviations

BA

6-Benzyl adenine

IAA

Indole-3-acetic acid

IBA

Indole-3-butyric acid

2iP

2-Isopentenyl adenine

Kn

6-Furfurylaminopurine

PGRs

Plant growth regulators

MS

Murashige and Skoog (1962) medium

NAA

α Naphthalene acetic acid

Notes

Acknowledgments

The award of DST, Young Scientist (SR/FT/LS-014/2009) Scheme to Naseem Ahmad by the Department of Science and Technology (DST), Government of India, New Delhi, is greatly acknowledged. Research support from the Department of Science and Technology (Govt. of India) New Delhi under the DST-FIST (2011) and UGC-SAP (2009) Programme, is also acknowledged.

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2012

Authors and Affiliations

  • Naseem Ahmad
    • 1
    Email author
  • Md Imran Khan
    • 1
  • Sarfaraz Ahmed
    • 2
  • Saad Bin Javed
    • 1
  • Mohammad Faisal
    • 3
  • Mohammad Anis
    • 1
  • Sumbul Rehman
    • 4
  • Syed Mohammad Umair
    • 4
  1. 1.Plant Biotechnology Laboratory, Department of BotanyAligarh Muslim UniversityAligarhIndia
  2. 2.Natural Product Laboratory, Department of ChemistryAligarh Muslim UniversityAligarhIndia
  3. 3.Department of Botany and Microbiology, College of ScienceKing Saud UniversityRiyadhKingdom of Saudi Arabia
  4. 4.Department of Ilmul Advia, Faculty of Unani MedicineAligarh Muslim UniversityAligarhIndia

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