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Acta Physiologiae Plantarum

, Volume 35, Issue 3, pp 749–761 | Cite as

Variability of phenolic composition and biological activities of two Tunisian halophyte species from contrasted regions

  • Najla TrabelsiEmail author
  • Pierre Waffo-Téguo
  • Mejdi Snoussi
  • Riadh Ksouri
  • Jean Michel Mérillon
  • Abderrazek Smaoui
  • Chedly Abdelly
Original Paper

Abstract

The present study consists in evaluating the inter- and intraspecific variability of phenolic contents and biological capacities of Limoniastrum monopetalum L. and L. guyonianum Boiss. extracts. Ultimately, they were subjected to HPLC for phenolic identification. Results showed a great variation of phenolic content as function of species and localities. In fact, L. guyonianum extracts (El Akarit) contained the highest polyphenol (57 mg GAE g−1 DW), flavonoid (9.47 mg CE g−1 DW) and condensed tannin contents (106.58 mg CE g−1 DW). These amounts were accompanied by the greatest total antioxidant activity (128.53 mg GAE g−1 DW), antiradical capacity (IC50 = 4.68 μg/ml) and reducing power (EC50 = 120 μg/ml). In addition, L. monopetalum and L. guyonianum extracts exhibited an important and variable antibacterial activity with a diameter of inhibition zone ranging from 6.00 to 14.83 mm. Furthermore, these extracts displayed considerable antifungal activity. L. monopetalum extracts (Enfidha) showed the strongest activity against Candida glabrata and C. krusei with a diameter exceeding 12 mm. The phytochemical investigation of these extracts confirmed the variability of phenolic composition, since the major phenolic compound varied as a function of species and locality. These findings suggest that these two halophytes may be a new source of natural antioxidants that are increasingly important for human consumption, as well as for agro-food, cosmetic and pharmaceutical industries.

Keywords

Limoniastrum species Provenances Phenolic contents Biological activities HPLC analysis 

Abbreviations

DPPH

2,2-Diphenyl-1-picrylhydrazyl

IC50

Inhibition concentration at 50 %

EC50

Effective concentration at which the absorbance was 0.5

BHT

Butylated hydroxytoluene

GAE

Gallic acid equivalents

CE

Catechin equivalents

MeOH

Methanol

TFA

Trifluoroacetic acid

MeCN

Acetonitrile

ATCC

American type culture collection

NCIMB

National Collection of Industrial Marine and Food Bacteria

DH

Department of Agriculture

CIP

Collection Institut Pasteur

MR

Methicillin resistant

EGCG

Epigallocatechin-3-O-gallate

Notes

Acknowledgments

This work was supported by the Tunisian Ministry of Higher Education and Scientific Research (LR10CBBC02), by the Tunisian–French “Comité Mixte de Coopération Universitaire” (CMCU) network # 08G0917 and by the SATREPES Project: “Valorization of Bio-resources in Semi-Arid and Arid Land for Regional Development”.

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2012

Authors and Affiliations

  • Najla Trabelsi
    • 1
    • 2
    Email author
  • Pierre Waffo-Téguo
    • 2
  • Mejdi Snoussi
    • 3
  • Riadh Ksouri
    • 1
  • Jean Michel Mérillon
    • 2
  • Abderrazek Smaoui
    • 1
  • Chedly Abdelly
    • 1
  1. 1.Laboratoire des Plantes Extrêmophiles, Centre de Biotechnologie de Borj CédriaHammam-LifTunisia
  2. 2.Laboratoire de Sciences Végétales, Mycologie et Biotechnologie. Groupe d’Etude des Substances Végétales à Activité Biologique (GESVAB) EA 3675, UFR PharmacieInstitut des Sciences de la Vigne et du Vin (ISVV), Université Victor Segalen Bordeaux 2BordeauxFrance
  3. 3.Laboratoire de Traitement et de Recyclage des EauxCentre de Recherches et des Technologies des Eaux, Technopole de Borj-CédriaHammam-LifTunisia

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