Skip to main content
Log in

In vitro propagation and assessment of genetic integrity of Talinum triangulare (Jacq.) Willd: a valuable medicinal herb

  • Original Paper
  • Published:
Acta Physiologiae Plantarum Aims and scope Submit manuscript

Abstract

Talinum triangulare is a medicinally important herb and various parts of the plant are used pharmaceutically for the treatment of different diseases. In our study, a rapid and efficient protocol for micropropagation has been developed from shoot tip and nodal explants of T. triangulare. High shooting frequency (93.33 %) was achieved with shoot tip explants when cultured on Murashige and Skoog’s (MS) medium supplemented with 1.0 mg/L 6-benzyl amino purine (BAP) producing an average of 12.50 ± 0.23 shoots and 5.07 ± 0.02 cm shoot length per explant. A combination of 0.5 mg/L BAP and 0.5 mg/L kinetin was found to be more effective by producing 15.67 ± 0.25 shoots and 6.22 ± 0.02 cm shoot length per explant. The microshoots were excised and cultured on half-strength MS and full-strength MS medium containing different concentrations of indole-3-acetic acid and indole-3-butyric acid (IBA) for root induction. More number of roots (45.10 ± 0.96) with an average length of 5.46 ± 0.08 cm was obtained on half-strength MS medium supplemented with 0.5 mg/L IBA. The rooted shoots were successfully transplanted from different planting substrates to the field with a 100 % survival rate. Random amplified polymorphic DNA analysis was carried out using four random decamer primers. The amplification products were monomorphic in the micropropagated plants and were similar to the mother plant. Absence of polymorphism revealed that no variation was induced, thus maintaining the genetic integrity of the micropropagated plants of T. triangulare.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Adefolaju GA, Ajao MS, Olatunji LA, Enaibe BU, Musa MG (2009) Hepatoprotective effect of aqueous extract of water leaf (Talinum triangulare) on carbon tetrachloride (CCl4) induced liver damage in wistar rats. Internet J Pathol 8(1):1–9

    Google Scholar 

  • Adewunmi AO, Sofowora EA (1980) Preliminary screening of some plant extracts for molluscicidal activity. Planta Med 39:57–82

    Article  Google Scholar 

  • Agble F (1970) Germination of seeds of Talinum triangulare. Ghana J Sci 10:29–32

    Google Scholar 

  • Andarwulan N, Batari R, Sandrasari DA, Bolling B, Wijaya H (2010) Flavonoid content and antioxidant activity of vegetables from Indonesia. Food Chem 121:1231–1235

    Article  CAS  Google Scholar 

  • Anonymous (1976) The wealth of India: a dictionary of raw materials and industrial products, vol X (Sp-W). CSIR, New Delhi, pp 113–114

  • Balaraju K, Agastian P, Preetamraj JP, Arokiyaraj S, Ignacimuthu S (2008) Micrpropagation of Vitex agnus-castus (Verbenaceae)—a valuable medical plant. In Vitro Cell Dev Biol Plant 44:436–441

    Article  CAS  Google Scholar 

  • Baskaran P, Jayabalan N (2005) An efficient micropropagation system for Eclipta alba—a valuable medicinal herb. In Vitro Cell Dev Biol Plant 41:532–539

    Article  CAS  Google Scholar 

  • Berlin J, Sieg S, Strack D, Bokern M, Harms H (1986) Production of betalains by suspension cultures of Chenopodium rubrum L. Plant Cell Tissue Organ Cult 5:163–174

    Article  CAS  Google Scholar 

  • Bianco-Colomas J, Hugues M (1990) Establishment and characterization of a betacyanin producing cell line of Amaranthus tricolor: inductive effects of light and cytokinin. J Plant Physiol 136:734–739

    Article  CAS  Google Scholar 

  • Biddington NL, Thomas TH (1973) A modified Amaranthus betacyanin bioassay for the rapid determination of cytokinins in plant extracts. Planta 111:183–186

    Article  CAS  Google Scholar 

  • Ceasar SA, Maxwell SL, Prasad KB, Karthigan M, Ignacimuthu S (2010) Highly efficient shoot regeneration of Bacopa monnieri (L.) using a two-stage culture procedure and assessment of genetic integrity of micropropagated plants by RAPD. Acta Physiol Plant 32:443–452

    Article  CAS  Google Scholar 

  • Chishti N, Sahwl AS, Kaloo ZA, Bhat MA, Sultan P (2006) Clonal propagation of Mentha arvensis L. through nodal explants. Pak J Biol Sci 9:1416–1419

    Article  CAS  Google Scholar 

  • Cronquist A (1981) An integrated system of classification of flowering plants. Columbia University Press, New York, pp 235–239

    Google Scholar 

  • Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation. Version II. Plant Mol Biol Rep 1:19–21

    Article  CAS  Google Scholar 

  • Escribano J, Pedreno MA, Garcia-Carmona F, Munoz R (1998) Characteristic of the antiradical activity of betalains from Beta vulgaris L. roots. Phytochem Anal 9:124–127

    Article  CAS  Google Scholar 

  • Ezekwe MO, Besong SA, Igbokwe PE, Ezekwe EI (2002) Beneficial influence of Purslane and Waterleaf supplementation on cardiovascular disease risk in humans. FASEB J 16(4):A639

    Google Scholar 

  • Fawusi MOA (1979) Germination of Talinum triangulare L. seeds as affected by various chemical and physical treatments. Ann Bot 44:617–622

    CAS  Google Scholar 

  • Fontem DA, Schippers RR (2004) Talinum triangulare (Jacq.) Willd [Internet] Record from Protabase: Grubben GJH, Denton OA (Editors). PROTA (Plant Resources of Tropical Africa/Ressources végétales de l’Afrique tropicale), Wageningen, Netherlands. http://database.prota.org/search.htm Accessed 20 Oct 2010

  • Frabetti M, Pesce PG, Gyves EM, Rugini E (2009) Micropropagation of Teucrium fruticans L., an ornamental and medicinal plant. In Vitro Cell Dev Biol Plant 45:129–134

    Article  CAS  Google Scholar 

  • Franca SC, Bertoni BW, Pereira AMS (1995) Anti-hepatotoxic agent in micropropagated plantlets of Eclipta alba. Plant Cell Tissue Organ Cult 40:297–299

    Article  CAS  Google Scholar 

  • George EF (1993) Plant propagation by tissue culture. Part I. The technology exegetics Ltd, Edignton

    Google Scholar 

  • Georgiev V, Ilieva M, Bley T, Pavlov A (2008) Betalain production in plant in vitro systems. Acta Physiol Plant 30:581–593

    Article  CAS  Google Scholar 

  • Guo B, Gao M, Liu CZ (2007) In vitro propagation of an endangered medicinal plant Saussurea involucrata Kar. et Kir. Plant Cell Rep 26:261–265

    Article  PubMed  CAS  Google Scholar 

  • Husain MK, Anis M (2006) Rapid in vitro propagation of Eclipta alba (L.) Hassk. through high frequency axillary shoot proliferation. Acta Physiol Plant 28(4):325–330

    Article  CAS  Google Scholar 

  • Indhra DB, Dhar U (2000) Micropropagation of Indian wild strawberry. Plant Cell Tissue Organ Cult 60:83–88

    Article  Google Scholar 

  • Keay RWJ (1981) Flora of west tropical Africa. Whitefriars Press Ltd., London

    Google Scholar 

  • Khare CP (2007) Indian medicinal plants an illustrated dictionary. Springer publication, New York, p 643

    Google Scholar 

  • Kimler LM (1975) Betanin, the red beet pigment as an antifungal agent. Botanical Society of America, Abstract of Paper 36

  • Kochhar VK, Kochhar S, Mohr H (1981) An analysis of the action of light on betalain synthesis in the seedling of Amaranthus caudatus var. viridis. Planta 151:81–87

    Article  CAS  Google Scholar 

  • Leathers RR, Davin C, Zryd JP (1992) Betalain producing cell cultures of Beta vulgaris L. var. bikores monogerm (red beet). In Vitro Cell Dev Biol Plant 28:39–45

    Google Scholar 

  • Liang D, Zhou Q, Gong W, Wang Y, Nie Z, He H, Li J, Wu J, Wu C, Zhang J (2011) Studies on the antioxidant and hepatoprotective activities of polysaccharides from Talinum triangulare. J Ethnopharmacol 136(2):316–321

    Article  PubMed  CAS  Google Scholar 

  • Murashige T, Skoog FA (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Neves LO, Tomaz L, Favereiro MPS (2001) Micropropogation of Medicago truncatula Gaertn. c.v. Jemalong and Medicago truncatula ssp. narbonensis. Plant Cell Tissue Organ Cult 67:81–84

    Article  CAS  Google Scholar 

  • Nwoke FIO (1982) Effects of photoperiod on germination of seeds of Talinum triangulare (Jacq.) Willd. Ann Bot 49:23–29

    Google Scholar 

  • Pattnaik SK, Chand PK (1996) In vitro propagation of the medicinal herbs Ocimum americanum L. syn. O. canum Sims (hoary basil) and Ocimum sanctum (holy basil). Plant Cell Rep 15:846–850

    Article  CAS  Google Scholar 

  • Rajeswari V, Paliwal K (2006) In vitro propagation of Albizia odoratissima L.F. (Benth.) from cotyledonary node and leaf nodal explants. In Vitro Cell Dev Biol Plant 42:399–404

    Article  CAS  Google Scholar 

  • Rani G, Talwar D, Nagpal A, Virk G (2006) Micropropagation of Coleus blumei from nodal segments and shoot tips. Biol Plant 50:496–500

    Article  CAS  Google Scholar 

  • Rout GR, Das G (2002) An assessment of genetic integrity of micropropagated plants of Plumbago zeylanica by RAPD markers. Biol Plant 45:27–32

    Article  CAS  Google Scholar 

  • Safdari Y, Kazemitabar SK (2009) Plant tissue culture study on two different races of purslane (Portulaca oleracea L.). Afr J Biotechnol 8:5906–5912

    CAS  Google Scholar 

  • Sakuta M, Takagi T, Komamine A (1986) Growth related accumulation of betacyanin in suspension cultures of Phytolacca americana L. J Plant Physiol 125:337–343

    Article  CAS  Google Scholar 

  • Selvi DN, George L, Eapen S (2002) Micropropagation and field evaluation of micropropagated plants of turmeric. Plant Cell Tissue Organ Cult 68:143–151

    Article  Google Scholar 

  • Singh SK, Rai MK, Sahoo L (2012) An improved and efficient micropropagation of Eclipta alba through transverse thin cell layer culture and assessment of clonal fidelity using RAPD analysis. Ind Crop Prod 37:328–333

    Article  CAS  Google Scholar 

  • Sridhar R, Lakshminarayana G (1993) Lipid classes, fatty acids and tocopherols of leaves of six edible plant species. J Agric Food Chem 41:61–63

    Article  CAS  Google Scholar 

  • Srivastava A, Joshi AG (2009) In vitro behaviour of nodal explants of Portulaca grandiflora under the influence of cytokinins. Acta Univ Latv 753:43–48

    Google Scholar 

  • Sujatha G, Kumari BDR (2007) Effect of phytohormones on micropropagation of Artemisia vulgaris L. Acta Physiol Plant 29:189–195

    Article  Google Scholar 

  • Thangavel K, Maridass M, Sasikala M, Ganesan V (2008) In vitro micropropagation of Talinum portulacifolium L. through axillary bud culture. Ethnobot Leafl 12:413–418

    Google Scholar 

  • Thirugnanasampandan R, Mahendran G, Bai YN (2010) High frequency in vitro propagation of Isodon wightii (Bentham) H. Hara. Acta Physiol Plant 32:405–409

    Article  Google Scholar 

  • Thomas TD, Yoichiro H (2010) In vitro propagation for the conservation of a rare medicinal plant Justicia gendarussa Burm. f. by nodal explants and shoot regeneration from callus. Acta Physiol Plant 32:943–950

    Article  Google Scholar 

  • Valladares S, Anchez CS, Martınez MT, Ballester A, Vieitez AM (2006) Plant regeneration through somatic embryogenesis from tissues of mature oak trees: true-to-type conformity of plantlets by RAPD analysis. Plant Cell Rep 25:879–886

    Article  PubMed  CAS  Google Scholar 

  • Venkatachalam LE, Sreedhar RV, Bhagyalakshmi N (2007) Micropropagation in banana using high levels of cytokinins does not involve any genetic changes as revealed by RAPD and ISSR markers. Plant Growth Regul 51:193–205

    Article  CAS  Google Scholar 

  • Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535

    Article  PubMed  CAS  Google Scholar 

  • Yan N, Hu H, Huang JL, Xu K, Wang H, Zhou ZK (2006) Micropropagation of Cypripedium flavum through multiple shoots of seedlings derived from multiple seeds. Plant Cell Tissue Organ Cult 84:114–118

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to R. Ravindhran.

Additional information

Communicated by B. Borkowska.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Swarna, J., Ravindhran, R. In vitro propagation and assessment of genetic integrity of Talinum triangulare (Jacq.) Willd: a valuable medicinal herb. Acta Physiol Plant 34, 1987–1996 (2012). https://doi.org/10.1007/s11738-012-0999-6

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11738-012-0999-6

Keywords

Navigation