Acta Physiologiae Plantarum

, Volume 34, Issue 5, pp 1935–1941 | Cite as

Salinity-induced expression of pyrrolline-5-carboxylate synthetase determine salinity tolerance in Brassica spp

  • K. Chakraborty
  • R. K. Sairam
  • R. C. Bhattacharya
Original Paper

Abstract

The objective of the present study was to assess the role of salinity-induced expression of pyrrolline 5-carboxylate synthetase (P5CS), P5CS activity, and proline accumulation on salinity tolerance in Brassica genotypes. A pot culture experiment was conducted with four Brassica genotypes viz. CS 52, CS 54, Varuna, (B. juncea) and T 9 (B. campestris) under control and two salinity levels, i.e., 1.65, 4.50 and 6.76 dS m−1. Proline contents increased with increasing levels of salinity, and the highest content were recorded at post-flowering stage in CS 52 and CS 54. Activity of P5CS recorded at flowering stage was highest at higher level of salinity, with CS 52 and CS 54 recording highest activity. Gene expression of P5CS, which regulates the synthesis of proline, was higher in CS 52 and CS 54 under salt stress than Varuna and T 9. Comparison of partial nucleotide as well as amino acid sequence showed conserved domains, and inter and intra generic relatedness of these genes. The study suggests that salinity-induced expression of P5CS, pyrrolline-phosphate synthetase activity and proline accumulation may serve as one of the mechanism of salinity stress tolerance in Brassica genotypes.

Keywords

Brassica Compatible solutes Gene expression Osmolytes Proline Pyrrolline 5-carboxylae synthetase Salinity stress 

Abbreviations

ECe

Electrical conductivity of extract

MSI

Membrane stability index

P5CS

Pyrrolline 5-carboxylae synthetase

RWC

Relative water content

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2012

Authors and Affiliations

  • K. Chakraborty
    • 1
  • R. K. Sairam
    • 1
  • R. C. Bhattacharya
    • 2
  1. 1.Division of Plant PhysiologyIndian Agriculture Research InstituteNew DelhiIndia
  2. 2.National Research Center on Plant BiotechnologyIndian Agriculture Research InstituteNew DelhiIndia

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