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Effects of cement dust on volatile oil constituents and antioxidative metabolism of Aleppo pine (Pinus halepensis) needles

Abstract

The effects of cement dust on the chemical composition of essential oil, lipid peroxidation and antioxidant enzyme activities of Aleppo pine (P. halepensis) needles were studied. Cement dust resulted in a significant decrease in the yield of essential oil with the effect being more pronounced in the close vicinity of the cement factory. A concomitant decrease in all components of the oil was observed and δ-2-carene, trans-carveol, trans-carvyl acetate, α-terpinyl acetate, β-copaene, (E,E)-α-farnesene, α-calacorene, α-cadinene, spathulenol, humulene oxide II, 8-epi-γ-eudesmol, Ί-muurolol, cubenol and ethyl hexadecanoate have been proposed as biological indicators of cement dust. Moreover, a redirection of the secondary metabolism toward the biosynthesis of monoterpenes has been evidenced. Malondialdehydes (MDA), a decomposition product of polyunsaturated fatty acids, often considered as a suitable biomarker for lipid peroxidation was induced in the needles exposed to cement dust. Similarly, a remarkable induction of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activities was noticed. The positive relationships were observed among activities of antioxidant enzymes, and between MDA content and activities of antioxidant enzymes, indicating the cooperative action of these antioxidant enzymes to cope with the oxidative stress induced by cement dust. The results obtained indicate that P. halepensis needles are useful bio-monitors of cement dust pollution.

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Acknowledgments

This work was supported by the Direction Générale de la Recherche Scientifique (DGRS, Tunisia) and the Centre National de la Recherche Scientifique (CNRS, France), Research Project 11/R 09-11. The authors are grateful to the anonymous reviewer for his helpful comments and suggestions.

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Correspondence to Karim Hosni.

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Communicated by J. V. Jorrin-Novo.

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Dziri, S., Hosni, K. Effects of cement dust on volatile oil constituents and antioxidative metabolism of Aleppo pine (Pinus halepensis) needles. Acta Physiol Plant 34, 1669–1678 (2012). https://doi.org/10.1007/s11738-012-0962-6

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  • DOI: https://doi.org/10.1007/s11738-012-0962-6

Keywords

  • Pinus halepensis
  • Cement dust
  • Essential oil
  • Malondialdehydes
  • Antioxidant enzymes