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Effects of fertilization and drought stress on tannin biosynthesis of Casuarina equisetifolia seedlings branchlets

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Abstract

Nutrient, water, and their interactions influence the allocation of investment by plants to resistance and tolerance traits. We used a completely crossed randomized-block design experiment to examine the independent and interactive effects of nutrients and water availability on tannin production of C. equisetifolia seedlings. The results showed that nitrogen and phosphorus fertilizer have significant effects on total phenolics (TP) and extractable condensed tannins (ECT) concentrations of branchlets. TP and ECT concentrations decreased with fertilizer addition and increased in arid condition. This pattern lends to support source-sink hypothesis such as the carbon-nutrient balance (CNB) hypothesis and the growth-differentiation balance (GDB) hypothesis. Soluble sugars or starch concentrations were both inversely related to TP concentrations. However, there was no significant correlation between them and ECT concentrations. In addition, chlorophyll concentration had a positive linear correlation with TP and no significant correlation with ECT. On the contrary, chlorophyll a/b ratios were negatively correlated with TP and positively correlated with ECT. The discrepancy of relationship between carbohydrates and TP or ECT showed that the biosynthetic routes of different tannins were different. In this study, no significant correlation between TP and N, or ECT and N, did not support protein competition model (PCM). TP:N and ECT:N ratios were higher in nutrient deficiency and arid conditions, which were one of the important nutrient conservation strategies for C. equisetifolia.

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Acknowledgments

This work was supported by the National Eleventh Five-Year Key Project (No. 2009BADB2B0302), One Hundred-Talent Plan of the Chinese Academy of Sciences (CAS), the National Natural Science Foundation of China (No. 41171216; 41001137; 31100313), the CAS/SAFEA International Partnership Program for Creative Research Teams, the Science & Technology Development Plan of Shandong Province (2010GSF10208),Yantai Science and Technology Development Project (2011016; 2010245), the Important Direction Project of CAS (KZCX2-YW-JC203) and the Strategic Priority Research Program of the Chinese Academy of Sciences(CAS)(XDA01020304) and Yantai Double-hundred Talent Plan.

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Authors and Affiliations

  1. Key Laboratory of Coastal Environmental Processes, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Yantai, 264003, People’s Republic of China

    Li Hua Zhang & Hong Bo Shao

  2. Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes, YICCAS, Yantai, 264003, People’s Republic of China

    Li Hua Zhang & Hong Bo Shao

  3. Institute of Life Sciences, Qingdao University of Science and Technology, Zhengzhou Rd.53, Qingdao, 266042, People’s Republic of China

    Hong Bo Shao

  4. Fujian Academy of Forestry, Fuzhou, 350012, People’s Republic of China

    Gong Fu Ye

  5. Key Lab of Coastal and Wetland Ecosystems, Ministry of Education, and School of Life Sciences, Xiamen University, Xiamen, 361005, People’s Republic of China

    Yi Ming Lin

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  1. Li Hua Zhang
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  2. Hong Bo Shao
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  3. Gong Fu Ye
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Correspondence to Hong Bo Shao or Gong Fu Ye.

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Communicated by W. Filek.

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Zhang, L.H., Shao, H.B., Ye, G.F. et al. Effects of fertilization and drought stress on tannin biosynthesis of Casuarina equisetifolia seedlings branchlets. Acta Physiol Plant 34, 1639–1649 (2012). https://doi.org/10.1007/s11738-012-0958-2

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