Abstract
Really interesting new gene (RING)-H2 proteins are widely represented in plants and play important roles in the regulation of many developmental processes as well as in plant–environment interactions. In the present report, a full-length cDNA encoding a putative RING-H2 protein with high sequence homology to Arabidopsis thaliana RING-H2 gene XERICO, designated as ZmXERICO, was isolated from maize. The full-length cDNA of ZmXERICO contained a 435 bp open reading frame encoding a precursor protein of 145 amino acid residues. Comparative and bioinformatics analyses revealed that ZmXERICO showed a high degree of homology with RING-H2 proteins and contained the transmembrane domain (TM) and RING-H2 motif. In our study, ZmXERICO was analyzed for expression profiles in various organs (root, stem, leaf, stamen, pistil and seed) and responding to exogenous hormone treatment and abiotic stresses using real-time PCR. The results showed that the expression of ZmXERICO was different regulated after treated by gibberellins acid-3 (GA3) and GA biosynthetic inhibitor paclobutrazol between in light and darkness. Moreover, the expression of ZmXERICO under different abiotic stresses was analyzed at different time points. The ZmXERICO was up-regulated in salt, drought, abscisic acid and cold stress. These expression results suggested that ZmXERICO played an important role in plant hormones signal pathways and light signal pathways, and that ZmXERICO might be involved in plant defense system against environmental stresses in maize.
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Abbreviations
- RING:
-
Really interesting new gene
- ORF:
-
Open reading frame
- ABA:
-
Abscisic acid
- GA:
-
Gibberellins
- PEG:
-
Polyethylene glycol
- PAC:
-
Paclobutrazol
References
Bopopi JM, Vandeputte OM, Himanen K, Mol A, Vaessen Q, El Jaziri M, Baucher M (2010) Ectopic expression of PtaRHE1, encoding a poplar RING-H2 protein with E3 ligase activity, alters plant development and induces defence-related responses. J Exp Bot 61:297–310
de Lucas M, Daviere JM, Rodriguez-Falcon M, Pontin M, Iglesias-Pedraz JM, Lorrain S, Fankhauser C, Blazquez MA, Titarenko E, Prat S (2008) A molecular framework for light and gibberellin control of cell elongation. Nature 451:480–484
Du QL, Cui WZ, Zhang CH, Yu DY (2010) GmRFP1 encodes a previously unknown RING-type E3 ubiquitin ligase in Soybean (Glycine max). Mol Biol Rep 37:685–693
Feng S, Martinez C, Gusmaroli G, Wang Y, Zhou J, Wang F, Chen L, Yu L, Iglesias-Pedraz JM, Kircher S, Schafer E, Fu X, Fan LM, Deng XW (2008) Coordinated regulation of Arabidopsis thaliana development by light and gibberellins. Nature 451:475–479
Freemont PS, Hanson IM, Trowsdale J (1991) A novel cysteine-rich sequence motif. Cell 64:483–484
Haglund K, Dikic Y (2005) Ubiquitylation and cell signaling. EMBO J 24:3353–3359
Jensen RB, Jensen KL, Jespersen HM, Skriver K (1998) Widespread occurrence of a highly conserved RING-H2 zinc finger motif in the model plant Arabidopsis thaliana. FEBS Lett 436:283–287
Joazeiro CA, Weissman AM (2000) RING finger proteins: mediators of ubiquitin ligase activity. Cell 102:549–552
Kam J, Gresshoff P, Shorter R, Xue GP (2007) Expression analysis of RING zinc finger genes from Triticum aestivum and identification of TaRZF70 that contains four RING-H2 domains and differentially responds to water deficit between leaf and root. Plant Sci 173:650–659
Katoh S, Tsunoda Y, Murata K, Minami E, Katoh E (2005) Active site residues and amino acid specificity of the ubiquitin carrier protein-binding RING-H2 finger domain. J Biol Chem 280:41015–41024
Ko JH, Yang SH, Han KH (2006) Upregulation of an Arabidopsis RING-H2 gene, XERICO, confers drought tolerance through increased abscisic acid biosynthesis. Plant Journal 47:343–355
Kumar S, Tamura K, Nei M (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163
Laity JH, Lee BM, Wright PE (2001) Zinc finger proteins: new insights into structural and functional diversity. Curr Opin Struct Biol 11:39–46
Liu HZ, Zhang HJ, Yang YY, Li GJ, Yang YX, Wang X, Vindhya BM, Basnayake S, Li DY, Song FM (2008) Functional analysis reveals pleiotropic effects of rice RING-H2 finger protein gene OsBIRF1 on regulation of growth and defense responses against abiotic and biotic stresses. Plant Mol Biol 68:17–30
Molnar G, Bancos S, Nagy F, Szekeres M (2002) Characterisation of BRH1, a brassinosteroid-responsive RING-H2 gene from Arabidopsis thaliana. Planta 215:127–133
Piskurewicz U, Jikumaru Y, Kinoshita N, Nambara E, Kamiya Y, Lopez-Molina L (2008) The gibberellic acid signaling repressor RGL2 inhibits Arabidopsis seed germination by stimulating abscisic acid synthesis and ABI5 activity. Plant Cell 20:2729–2745
Sahin-Cevik M, Moore GA (2006) Isolation and characterization of a novel RING-H2 finger gene induced in response to cold and drought in the interfertile Citrus relative Poncirus trifoliata. Physiol Plant 126:153–161
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
Saurin AJ, Borden KLB, Boddy MN, Freemont PS (1996) Does this have a familiar RING? Trends Biochem Sci 21:208–214
Schwechheimer C, Willige BC, Zourelidou M, Dohmann EMN (2009) Examining protein stability and its relevance for plant growth and development. Methods Mol Biol 479:147–171
Spartz AK, Gray WM (2008) Plant hormone receptors: new perceptions. Genes Dev 22:2139–2148
Stone SL, Hauksdottir H, Troy A, Herschleb J, Kraft E, Callis J (2005) Functional analysis of the RING-type ubiquitin ligase family of Arabidopsis. Plant Physiol 137:13–30
Zentella R, Zhang ZL, Park M, Thomas SG, Endo A, Murase K, Fleet CM, Jikumaru Y, Nambara E, Kamiya Y, Sun TP (2007) Global analysis of della direct targets in early gibberellin signalling in Arabidopsis. Plant Cell 19:3037–3057
Zhu JK (2002) Salt and drought stress signal transduction in plants. Annu Rev Plant Biol 53:247–273
Acknowledgments
This work was supported by National Natural Science Foundation of China (No. 31101093), Natural science Research Project for colleges and universities in Jiangsu Province (No.11KJB210005) and National Program on Key Basic Research Project (973 Program, 2009CB118400). We also thank another financial support for the Priority Academic Program Development of Jiangsu Higher Education Institutions.
Author contribution
The work presented here was carried out in collaboration between all authors. Yong Gao defined the research theme. Yong Gao and Hua Li designed methods and experiments, carried out the gene cloning and Real-time PCR experiments. Yong Gao and Jianmin Chen analyzed the data, interpreted the results and wrote the paper. Dexiang Deng provided seeds of maize. Shiqiang Chen and Wei Jiang co-worked the stress treatment and hormone treatment experiments. Jianmin Chen co-designed experiments, discussed analyses, interpretation, and presentation. All authors have contributed to, seen and approved the manuscript.
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Communicated by Y. Wang.
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Gao, Y., Li, H., Deng, D. et al. Characterization and expression analysis of the maize RING-H2 finger protein gene ZmXERICO responsive to plant hormones and abiotic stresses. Acta Physiol Plant 34, 1529–1535 (2012). https://doi.org/10.1007/s11738-012-0951-9
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DOI: https://doi.org/10.1007/s11738-012-0951-9