Acta Physiologiae Plantarum

, Volume 34, Issue 4, pp 1345–1351 | Cite as

Effects of sucrose concentration and exogenous hormones on growth and periplocin accumulation in adventitious roots of Periploca sepium Bunge

  • Jian Zhang
  • Wen-Yuan GaoEmail author
  • Juan Wang
  • Xing-lin Li
Original Paper


Periploca sepium adventitious roots were cultured on 0.5 Murashige and Skoog solid media supplemented with exogenous hormones of different types and various concentrations, and with sucrose of different concentrations. Auxins (indole butyric acid (IBA) and naphthalene acetic acid (NAA)) and cytokinins (6-benzylaminopurine (BA) and kinetin (KT)) were selected as exogenous hormones for adventitious root proliferation. Compared with other hormones, IBA was the suitable auxin for adventitious root proliferation. Under this circumstance, every root explant generates 10–15 adventitious roots (1- to 2-cm long) after 30 days. However, nothing but callus was induced on the root explants when NAA was added into the medium and the same result was achieved when auxins (IBA or NAA) were added into the media together with cytokinins (BA or KT). The suitable concentration of IBA for adventitious root proliferation was 1–2 mg/l, when every root explant generated 10–20 adventitious roots (1- to 2-cm long). The optimum concentration of IBA for periplocin accumulation was 1 mg/l, when the periplocin content reached 95.46 μg/g. With regard to the investigation of sucrose concentration, 2–3% (w/v) sucrose was favorable for adventitious root proliferation as every root explant in this concentration generated 10–20 adventitious roots (1- to 2-cm long). The highest periplocin content (101.56 μg/g) was achieved at 5% (w/v) sucrose, whereas the periplocin content at 5% (w/v) sucrose did not show significant difference from the periplocin content (95.38 and 98.47 μg/g, respectively) at 3% (w/v) or 4% (w/v) sucrose.


Periploca sepium Bunge Adventitious root proliferation Exogenous hormones Sucrose concentration Periplocin 



Murashige and Skoog


Indole butyric acid


Naphthalene acetic acid






Lactate dehydrogenase



This research was financially supported by the Main Project of Tianjin Science and Technology Support Program (09ZCKFSH01100).


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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2012

Authors and Affiliations

  • Jian Zhang
    • 1
  • Wen-Yuan Gao
    • 2
    Email author
  • Juan Wang
    • 2
  • Xing-lin Li
    • 3
  1. 1.School of Traditional Chinese Materia MedicaTianjin University of Traditional Chinese MedicineTianjinPeople’s Republic of China
  2. 2.School of Pharmaceutical Science and TechnologyTianjin UniversityTianjinPeople’s Repubic of China
  3. 3.Institute of Biological Engineering of Traditional Chinese MedicineTianjin University of Science and TechnologyTianjinPeople’s Republic of China

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