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Enhanced production of vasicine in Adhatoda vasica (L.) Nees. cell culture by elicitation

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Abstract

Vasicine, isolated from the shrub Adhatoda vasica, is a well-known pyrroloquinazoline alkaloidal compound which has been used for the treatment of various diseases and disorders, mostly for the respiratory-tract ailments. To counter the present low content in leaves and uneconomical chemical synthesis, alternate ways to produce vasicine have been required; but vasicine remains subtle in cell cultures of A. vasica despite the wide studies undertaken. This work reports the successful approach for production of vasicine by cell cultures of A. vasica. High and fast-growing leaf-derived cell culture was established from A. vasica. In the present study, elicitation as the yield enhancement strategy was developed by the addition of selected elicitor like methyl jasmonate (MeJ), chitosan, yeast extract, ascorbic acid, and sodium salicylate at optimized concentrations. This resulted in 0.45 and 0.39% (on dry weight basis) vasicine, which was 3.7 and 3.2-fold higher yield over the control cultures (0.121%) with 20 μM MeJ and 50 mg l−1 yeast extract treatment, respectively.

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Fig. 1
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Abbreviations

2,4-D:

2,4-Dichlorophenoxyacetic acid

AA:

Ascorbic acid

BAP:

Benzyl amino purine

CT:

Chitosan

DW:

Dry weight

GB5:

Gamborg B5

IBA:

Indole-3-butyric acid

Kn:

Kinetin

MeJ:

Methyl jasmonate

MS:

Murashige and Skoog

NAA:

1-Napthaleneacetic acid

PGR:

Plant growth regulator

SS:

Sodium salicylate

YE:

Yeast extract

References

  • Atal CK (1980) Chemistry and pharmacology of vasicine: an oxytocic and abortifacient. Regional Research Laboratory, Jammu-Tawi

    Google Scholar 

  • Azad MAK, Amin MN, Begum F (2003) Rapid clonal propagation of Adhatoda vasica Nees by tissue culture technique. Online J Biol Sci 3:172–182

    Google Scholar 

  • Bagchi GD, Dwivedi PD, Haider F, Singh S, Srivastava S, Chattopadhyay SK (2003) Seasonal variation in vasicine content in Adhatoda species grown under north Indian plain conditions. J Med Aromat Plant Sci 25:37–40

    CAS  Google Scholar 

  • Baldi A, Dixit VK (2008) Yield enhancement strategies for artemisinin production by suspension cultures of Artemisia annua. Bioresour Technol 99:4609–4614

    Article  PubMed  CAS  Google Scholar 

  • Conklin PL (2001) Recent advances in the role and biosynthesis of ascorbic acid in plants. Plant Cell Environ 24:383–394

    Article  CAS  Google Scholar 

  • Das C, Poi R, Chowdhury A (2005) HPTLC determination of vasicine and vasicinone in Adhatoda vasica. Phytochem Anal 16:90–92

    Article  PubMed  CAS  Google Scholar 

  • Dong X (1998) SA, JA, ethylene, and disease resistance in plants. Curr Opin Plant Biol 1:316–323

    Google Scholar 

  • Durner J, Shah J, Klessig DF (1997) Salicylic acid and disease resistance in plants. Trends Plant Sci 6:266–274

    Article  Google Scholar 

  • Ellard-lvey M, Douglas CJ (1996) Role of jasmonates in the elicitor- and wound inducible expression of defense genes in Parsley and transgenic Tobacco. Plant Physiol 112:183–192

    Google Scholar 

  • Fang Y, Smith MAL, Pepin MF (1999) Effects of exogenous methyl jasmonate in elicited anthocyanin producing cell cultures of Ohelo (Vaccinium pahalae). In Vitro Cell Dev Biol Plant 35:106–113

    Article  CAS  Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension culture of soybean root cells. Exp Cell Res 50:151–158

    Article  PubMed  CAS  Google Scholar 

  • Grange JM, Snell NJ (1996) Activity of bromohexine and ambroxiol, semi synthetic derivatives of vaccine from the Indian shrub Adhatoda vasica, against Mycobacterium tuberculosis in vitro. J Ethno Pharmacol 50:49–53

    CAS  Google Scholar 

  • Gupta OP, Sharma ML, Ghatak BJR, Atal CK (1977) Pharmacological investigations of vasicine and vasicinone- the alkaloids of Adhatoda vasica. Indian J Med Res 66:680–691

    PubMed  CAS  Google Scholar 

  • Gupta AK, Tandon N, Sharma M (eds) (2005) Quality standards of Indian medicinal plants vol 3, Indian Council of Medical Research, New Delhi, pp 1–7

  • Jaiswal VS, Narayan P, Lal M (1989) Micropropagation of Adhatoda vasica Nees, through nodal segments culture. In: Kukreja AK, Mathur AK, Ahuja PS, Thakur S (eds) Tissue culture and biotechnology of medicinal and aromatic plants. CIMAP, Lucknow, pp 7–11

    Google Scholar 

  • Jayapaul K, Kavikishor PB, Reddy KJ (2005) Production of pyrroloquinazoline alkaloid from leaf and petiole-derived callus cultures of A. zeylanica. In Vitro Cell Dev Biol Plant 41:682–685

    Article  CAS  Google Scholar 

  • Kahl G (1983) Wound repair and tumor induction in higher plants. In: Akazawa T, Imasei H (eds) The new frontiers in plants biochemistry. Japan Scientific Society Press Tokyo and Martinus Nijhoff/Dr. W. Junk Publishers, The Hague, pp 193–216

    Google Scholar 

  • Karwasara VS, Jain R, Tomar P, Dixit VK (2010) Elicitation as yield enhancement strategy for glycyrrhizin production by cell cultures of Abrus precatorius Linn. In Vitro Cell Dev Biol-Plant 46:354–362

    Article  CAS  Google Scholar 

  • Karwasara VS, Tomar P, Dixit VK (2011a) Oxytocin influences the production of glycyrrhizin from cell cultures of Abrus precatorius Linn. Plant Growth Regul 65:401–405

    Article  CAS  Google Scholar 

  • Karwasara VS, Tomar P, Dixit VK (2011b) Influence of fungal elicitation on glycyrrhizin production in transformed cell cultures of Abrus precatorius Linn. Pharmacogn Mag 7:307–313

    Article  PubMed  CAS  Google Scholar 

  • Khalekuzzaman M, Rahman MS, Rashid MH, Hossain MS (2008) High frequency in vitro propagation of Adhatoda vasica Nees through shoot tip and nodal explants culture. J Bio-sci 16:35–39

    Google Scholar 

  • Komaraiah P, Amrutha RN, Kishor PBK, Ramakrishna SV (2002) Elicitor enhanced production of plumbagin in suspension cultures of Plumbago rosea L. Enzym Microb Technol 31:634–639

    Article  CAS  Google Scholar 

  • Lahiri PK, Pradhan SN (1964) Pharmacological investigations of vasicinol: an alkaloid from Adhatoda vasica Nees. Ind J Exp Biol 2:219–223

    Google Scholar 

  • Liso R, Calahrese G, Bitonti MB, Arrigoni O (1984) Relationship between ascorbic acid and cell division. Exp Cell Res 150:314–320

    Article  PubMed  CAS  Google Scholar 

  • Malamy J, Sanchez-Casas P, Hennig J, Guo A, Klessig D (1996) Discussion of the salicylic acid signaling pathway in tobacco. Mol Plant Microb Interact 9:474–482

    Article  CAS  Google Scholar 

  • Mathew AS, Patel KN, Shah BK (1998) Investigation on antifeedant and antihelmintic potential of Adhatoda vasica Nees. Ind J Nat Prod 14:11–16

    Google Scholar 

  • Mueller MJ, Brodschelm W, Spannagl E, Zenk MH (1993) Signaling in the elicitation process is mediated through the octadecanoid pathway leading to jasmonic acid. Proc Natl Acad Sci USA 90:7490–7494

    Article  PubMed  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco cell cultures. Physiol Planta 15:473–479

    Article  CAS  Google Scholar 

  • Nath S, Buragohain AK (2004) Micropropagation of Adhatoda vasica Nees- A woody medicinal plant by shoot tip culture. Ind J Biotech 4:396–399

    Google Scholar 

  • Orlita A, Sidwa-Gorycka M, Paszkiewicz M, Mallinski E, Kumirska J, Siedlecka EM, Lojkowska E, Stepnowski P (2008) Application of chitin and chitosan as elicitors of coumarins and fluoroquinolone alkaloids in Ruta graveolens L. (common rue). Biotechnol Appl Biochem 51:91–96

    Article  PubMed  CAS  Google Scholar 

  • Patel PK, Bhatt PH (1984) In vitro study of antimicrobial activity of Adhatoda vasica (leaf extract) on gingival inflammation of preliminary report. Ind J Med Sci 38:70–72

    CAS  Google Scholar 

  • Pitta-Alvarez SI, Spollansky TC, Giulietti AM (2000) The influence of different biotic and abiotic elicitors on the production and profile of tropane alkaloids in hairy root cultures of Brugmansia candida. Enzym Microb Technol 26:252–258

    Article  CAS  Google Scholar 

  • Repka V, Fischerova I, Silharova K (2001) Methyl jasmonate is a potent elicitor of multiple defense responses in grapevine leaves and cell-suspension cultures. Biol Planta 48:273–283

    Article  Google Scholar 

  • Robert AC, John EM (1997) Biosynthesis and action of jasmonates in plants. Plant Physiol Plant Mol Biol 48:355–381

    Article  Google Scholar 

  • Roja G, Vikrant BH, Sandur SK, Sharma A, Pushpa KK (2011) Accumulation of vasicine and vasicinone in tissue cultures of Adhatoda vasica and evaluation of the free radical-scavenging activities of the various crude extracts. Food Chem 126:1033–1038

    Article  CAS  Google Scholar 

  • Shalaka DK, Parameswaran S (2009) Micropropogation and organogenesis in Adhatoda vasica for the estimation of vasicine. Phcog Mag 5:359–363

    Article  Google Scholar 

  • Shohael AM, Murthy HN, Hahn EJ, Paek KY (2007) Methyl jasmonate induced overproduction of eleutherosides in somatic embryos of Eleutherococcus senticosus cultured in bioreactors. Electron J Biotechnol 10:633–637

    Article  Google Scholar 

  • Shualev V, Leon J, Raskin I (1995) Is salicylic acid a translocated signal of systemic acquired resistance in tobacco? Plant Cell 7:1691–1701

    Google Scholar 

  • Smirnoff N, Wheeler GL (2000) Ascorbic acid in plants: biosynthesis and functions. Crit Rev Biochem Mol Biol 35:291–314

    Article  PubMed  CAS  Google Scholar 

  • Suzuki T, Mori H, Yamame T, Shimuzu S (1985) Automatic supplementation of minerals in fed-batch culture to high cell mass concentration. Biotech Bioeng 27:192–201

    Article  CAS  Google Scholar 

  • The wealth of India: a dictionary of Indian raw materials and industrial products (2003), vol A, NISCAIR, Council of Scientific and Industrial Research, New Delhi, pp 76–79

  • Walker TS, Bais HP, Vivanco JM (2002) Jasmonic acid induced hypericin production in Hypericum perforatum L. (St. John wort). Phytochem 60:289–293

    Article  CAS  Google Scholar 

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Acknowledgments

The author VSK wishes to acknowledge University Grant Commission (Basic Scientific Research-Meritorious Research Fellowship, grant letter No. F.4-1/2006-BSR/7-57/2007-BSR) New Delhi, India for providing research fellowship. The authors are thankful to Mr. G. Trimurtulu, Laila Impex Research Centre, Vijayawada, Andhra Pradesh, India for the generous gift sample of standard vasicine.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Biotechnology, Dr. Hari Singh Gour University, Sagar, 470 003, Madhya Pradesh, India

    Sweta Bhambhani & Smita Banerjee

  2. Department of Pharmaceutical Sciences, Dr. Hari Singh Gour University, Sagar, 470 003, Madhya Pradesh, India

    Vijai Singh Karwasara & Vinod Kumar Dixit

Authors
  1. Sweta Bhambhani
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  2. Vijai Singh Karwasara
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  3. Vinod Kumar Dixit
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  4. Smita Banerjee
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Corresponding author

Correspondence to Vinod Kumar Dixit.

Additional information

Communicated by Z.-L. Zhang.

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Bhambhani, S., Karwasara, V.S., Dixit, V.K. et al. Enhanced production of vasicine in Adhatoda vasica (L.) Nees. cell culture by elicitation. Acta Physiol Plant 34, 1571–1578 (2012). https://doi.org/10.1007/s11738-011-0921-7

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  • DOI: https://doi.org/10.1007/s11738-011-0921-7

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