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Effect of triadimefon: a triazole fungicide on oxidative stress defense system and eugenol content in Ocimum tenuiflorum L.


The effect of triadimefon (TDM) a triazole compound on antioxidant potential and eugenol content was studied in Ocimum tenuiflorum L. The plant was subjected to 15 mg/l TDM by soil-drenching on 50th, 70th and 90th days after planting (DAP). Analyses were carried out on 60th, 80th and 100th DAP. Both enzymatic and non-enzymatic antioxidant potential estimation was carried out. The non-enzymatic antioxidant viz ascorbic acid, α-tocopherol and reduced glutathione were found to increase under triadimefon treatment. The antioxidant enzyme like superoxide dismutase, ascorbate peroxidase and catalase activities showed significant increase in TDM treatment. Triazole-treated plants yielded more eugenol (a major component of Ocimum-essential oil) in comparison to control. The study evidenced that TDM residues could be detected in the upper soil layer only and does not accumulate in the soil. These preliminary results suggest that TDM may be useful to increase the antioxidant content in medicinal plants and also to act as an elicitor to enhance the production of secondary metabolites of medicinal plants.

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Fig. 1
Fig. 2





Ascorbic acid


Ascorbate peroxidase


Butylated hydroxy anisole


Butylated hydroxy toluene




Days after planting


Dry weight


Fresh weight


Farmyard manure


Reduced glutathione


Milligram per litre


Propyl gallate


Plant growth regulator


Random block design


Superoxide dismutase




Thin layer chromatography


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Correspondence to V. Divya Nair.

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Communicated by G. Bartosz.

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Divya Nair, V., Gopi, R., Mohankumar, M. et al. Effect of triadimefon: a triazole fungicide on oxidative stress defense system and eugenol content in Ocimum tenuiflorum L.. Acta Physiol Plant 34, 599–605 (2012).

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