Skip to main content
SpringerLink
Log in

Effect of triadimefon: a triazole fungicide on oxidative stress defense system and eugenol content in Ocimum tenuiflorum L.

Acta Physiologiae Plantarum volume 34, pages 599–605 (2012)Cite this article

Abstract

The effect of triadimefon (TDM) a triazole compound on antioxidant potential and eugenol content was studied in Ocimum tenuiflorum L. The plant was subjected to 15 mg/l TDM by soil-drenching on 50th, 70th and 90th days after planting (DAP). Analyses were carried out on 60th, 80th and 100th DAP. Both enzymatic and non-enzymatic antioxidant potential estimation was carried out. The non-enzymatic antioxidant viz ascorbic acid, α-tocopherol and reduced glutathione were found to increase under triadimefon treatment. The antioxidant enzyme like superoxide dismutase, ascorbate peroxidase and catalase activities showed significant increase in TDM treatment. Triazole-treated plants yielded more eugenol (a major component of Ocimum-essential oil) in comparison to control. The study evidenced that TDM residues could be detected in the upper soil layer only and does not accumulate in the soil. These preliminary results suggest that TDM may be useful to increase the antioxidant content in medicinal plants and also to act as an elicitor to enhance the production of secondary metabolites of medicinal plants.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Fig. 1
Fig. 2

Abbreviations

α-toc:

α-Tocopherol

AA:

Ascorbic acid

APX:

Ascorbate peroxidase

BHA:

Butylated hydroxy anisole

BHT:

Butylated hydroxy toluene

CAT:

Catalase

DAP:

Days after planting

DW:

Dry weight

FW:

Fresh weight

FYM:

Farmyard manure

GSH:

Reduced glutathione

mg/l:

Milligram per litre

PG:

Propyl gallate

PGR:

Plant growth regulator

RBD:

Random block design

SOD:

Superoxide dismutase

TDM:

Triadimefon

TLC:

Thin layer chromatography

References

  • Alscher RG, Donbahue JL, Cramer CL (1997) Reactive oxygen species and antioxidants relationships in green cells. Physiol Plant 100:224–233

    Article  CAS  Google Scholar 

  • Anna P, Fiorella P, Mattia L, Russo A, Gaeta L, Maria L, Giulia T, Giorgio F (2003) Determination of blood total, reduced, and oxidized glutathione in paediatric subjects. Clin Chem 47:1467–1469

    Google Scholar 

  • Archana R, Namasivayam A (2000) Effect of Ocimum sanctum on noise induced changes in neutrophil functions. J Ethnol Pharmacol 73:81–85

    CAS  Google Scholar 

  • Asada K (1992) Ascorbate peroxidase: a hydrogen peroxide-scavenging enzyme in plants. Physiol Plant 85:235–241

    Article  CAS  Google Scholar 

  • Asada K, Takahashi M (1987) Production and scavenging of active oxygen in photosynthesis. In: Kyle DJ, Osmond B, Arntzen CJ (eds) Photoinhibition. Elsevier, Amsterdam, pp 227–287

    Google Scholar 

  • Backer H, Frank O, De Angells B, Feingold O (1980) Plasmatocopherol in man at various times after ingesting free or acetylated tocopherol. Nutr Rep Int 21:531–536

    Google Scholar 

  • Beauchamp C, Fridovich I (1971) Superoxide dismutase improve assays and an essay applicable to acetylamide gels. Ann Biochem 44:276–287

    Article  CAS  Google Scholar 

  • Berova M, Zlateu Z, Stoeva N (2002) Effect of paclobutrazol on wheat seedlings under low temperature stress. Bulg J Plant Physiol 28:75–84

    CAS  Google Scholar 

  • Bhattacharjee S (2008) Triadimefon pre-treatment protects newly assembled membrane system and causes up-regulation of stress proteins in salinity stressed Amaranathus lividus L. during early germination. J Environ Biol 29:805–810

    PubMed  CAS  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for quantification of microgram quantities of proteins utilizing the principle of protein dye binding. Ann Biochem 72:248–253

    Article  CAS  Google Scholar 

  • Bromillow RH, Evans AA, Nicholls PH (1999) Factors affecting dissipation rates of five triazole fungicides in soil types: 2. field studies. Pestic Sci 55:1129–1142

    Google Scholar 

  • Burritt DJ, MacKenzie S (2003) Antioxidant metabolism during acclimation of Begonia × erythrophylla to high light levels. Ann Bot 91:783–794

    Article  PubMed  CAS  Google Scholar 

  • Chandlee JM, Scandalios JG (1984) Analysis of variants affecting the catalase development program in Maize scutellum. Theor Appl Genet 69:71–77

    Article  CAS  Google Scholar 

  • Chelikani P, Fita I, Loewen PC (2004) Diversity of structures and properties among catalases. Cell Mol Life Sci 61:192–208

    Article  PubMed  CAS  Google Scholar 

  • Chu C, Lin HT, Wong SS, Li GC (2003) Dissipation and distribution of difenoconazole in a model paddy field ecosystem. Plant Prot Bull 45:225–235

    CAS  Google Scholar 

  • Del Río L, Sandalio L, Palma J, Bueno P, Corpas F (1992) Metabolism of oxygen radicals in peroxisomes and cellular implications. Free Radic Biol Med 13:557–580

    Article  PubMed  Google Scholar 

  • Dixon RA, Paiva NL (1995) Stress induced phenyl propanoid metabolism. Plant Cell 7:1085–1097

    Article  PubMed  CAS  Google Scholar 

  • Dorman HJ, Surai P, Deans SG (2000) In vitro antioxidant activity of a number of plant essential oils and phytoconstituents. J Essent Oil Res 12:241–248

    CAS  Google Scholar 

  • Fletcher RA, Hofstra G (1988) Triazole as potential plant protectants. In: Berg D, Plample M (eds) Sterol Biosynthesis Inhibitors. Ellis Horwood Ltd., Cambridge, pp 321–331

    Google Scholar 

  • Fletcher RA, Gill A, Davis TD, Shankhala N (2000) Triazole as plant growth regulators and stress protectants. Horticult Rev 24:55–138

    CAS  Google Scholar 

  • Fujisawa S, Atsumi T, Kadoma Y, Sakagami H (2002) Antioxidant and prooxidant action of eugenol-related compounds and their cytotoxicity. Toxicology 177:35–54

    Article  Google Scholar 

  • Griffith OW, Meister A (1979) Potent and specific inhibition of glutathione synthesis by buthionine sulfoximine (S-n-butylmonocysteine sulfoximine). J Bio Chem 254:7558–7560

    CAS  Google Scholar 

  • Gupta SK, Raghava RP, Raghava N (2004) Stomatal studies of cowpea (Vigna unguicualta (L.) Walp) cultivars in relation to bromiconazole. J Indian Bot Soc 83:116–119

    Google Scholar 

  • Havaux M, Lutz C, Grimm B (2003) Chloroplast membrane photostability in chlp transgenic tobacco plants deficient in tocopherols. Plant Physiol 132:300–310

    Article  PubMed  CAS  Google Scholar 

  • Hwang SY, Lin HW, Chern RH, Lo HF, Li L (1999) Reduce susceptibility to water logging together with high light stress is related to increase in superoxide dismutase and catalase activity in sweet potato. Plant Growth Regul 27:167–172

    Article  CAS  Google Scholar 

  • Jain SK, Singh R, Misra KK (2002) Effect of paclobutrazol on growth yield-and fruit quality of lemon (Citrus lemon). Indian J Agr Sci 72:488–489

    Google Scholar 

  • Koleva II, Neederlander HAG, Van Beek TA (2000) An online HPLC method for detection of radical scavenging compounds in complex mixtures. Anal Chem 72:2323–2328

    Article  PubMed  CAS  Google Scholar 

  • Lin CC, Kao CH (2000) Effect of NaCl stress on H2O2 metabolism in rice leaves. Plant Growth Regul 30:151–155

    Article  CAS  Google Scholar 

  • Liu R, Xu S, Li J, Hu Y, Lin Z (2006) Expression profile of a PAL gene from Astrgalus membraceous var Mongholicus and its crucial role in flux into flavonoid biosynthesis. Plant Cell Rep 25:705–710

    Article  PubMed  CAS  Google Scholar 

  • Maxwell PJ, Praveen KS, Susan JM (2009) Elicitation of secondary metabolism in Echinarea purpurea L. by gibberellic acid and triazoles. Eng Life Sci 9:205–210

    Article  Google Scholar 

  • Meister (1994) Glutathione, ascorbate and cellular protection. Cancer Res 54:1969S–1975S

    PubMed  CAS  Google Scholar 

  • Omay ST, Turnbull JD, Sauberillich HE (1979) Selected methods for the determination of ascorbic acid in animal cells tissues and fluids. methods in enzymology. Academic Press, New York, pp 3–11

    Google Scholar 

  • Pastori G, Mullineaux P, Foyer CH (2000) Post transcriptional regulation prevents accumulation of glutathione reductase protein activity in the bundle sheath cells of maize, implication of the sensitivity of maize to low temperature. Plant Physiol 122:667–675

    Article  PubMed  CAS  Google Scholar 

  • Phippen WB, Simon JE (2000) Anthocyanin inheritance and instability in purple basil (Ocimum bascilicum L.). J Hard 91:289–296

    CAS  Google Scholar 

  • Pompella A, Visvikis A, Paolicchi A, De Tata V, Casini AF (2003) The changing faces of glutathione, a cellular protagonist. Biochem Pharmacol 66:1499–1503

    Article  PubMed  CAS  Google Scholar 

  • Rueegg J, Siegfried W (1996) Residues of difenoconazole and penconazole on apple leaves and grass and soil in an apple orchard in north-eastern Switzerland. Crop Prot 15:27–31

    Article  CAS  Google Scholar 

  • Scandalios JG, Guan L, Polindoros AN (1997) Catalase in plants, gene structure, properties, regulation and expression. In: Scandalios JG (ed) Oxidative stress and the molecular biology of antioxidant defenses. Cold Spring Harbor Laboratory, New York, pp 343–406

    Google Scholar 

  • Senaratna T, Mackay CE, McKersie BD, Fletcher RA (1988) Uniconazole induced chilling tolerance in tomato and its relationship to antioxidant content. J Plant Physiol 133:56–61

    Article  CAS  Google Scholar 

  • Shim IS, Momose Y, Yamamoto AH, Kim DWA, Usui K (2003) Inhibition of catalase activity by oxidative stress and its relationship to salicylic acid accumulation in plants. Plant Growth Regul 39:285–292

    Article  CAS  Google Scholar 

  • Slamenova D, Horvathova E, Wsolova L, Srankova M, Navarova J (2009) Investigaton of anti-oxidative cytotoxic, DNA-damaging and DNA-protective effects of plant volatiles eugenol and borneol in human-derived HepG2, CaCO-2 and VHIO cell lines. Mutat Res 677:46–52

    PubMed  CAS  Google Scholar 

  • Smirnoff N, Wheeler GL (2000) Ascorbic acid in plants: biosynthesis and function. Crit Rev Biochem Mol Biol 35:291–314

    Article  PubMed  CAS  Google Scholar 

  • Srivalli B, Chinnuswamy V, Chopra RK (2003) Antioxidant defense in response to abiotic stresses in plants. J Plant Biol 30:121–139

    Google Scholar 

  • Surveswaran S, Cai Yi-Z, Corke H, Sun M (2007) Systematic evaluation of natural phenolic antioxidants from 133 Indian medicinal plants. Food Chem 102:938–953

    Article  CAS  Google Scholar 

  • Xiongai Yi, Yitong Lu (2006) Residues and dynamics of probenconazole in rice field ecosystem. Chemosphere 65:639–643

    Article  Google Scholar 

  • Zamocky M, Koller F (1999) Understanding the structure and function of catalases: clues from molecular evolution and in vitro mutagenesis. Prog Biophys Mol Biol 72:19–66

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Stress Physiology Laboratory, Department of Botany, Annamalai University, Annamalainagar, Chidambaram, 608 002, Tamil Nadu, India

    V. Divya Nair, R. Gopi, M. Mohankumar, J. Kavina & R. Panneerselvam

Authors
  1. V. Divya Nair
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. Gopi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Mohankumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. Kavina
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. R. Panneerselvam
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to V. Divya Nair.

Additional information

Communicated by G. Bartosz.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Divya Nair, V., Gopi, R., Mohankumar, M. et al. Effect of triadimefon: a triazole fungicide on oxidative stress defense system and eugenol content in Ocimum tenuiflorum L.. Acta Physiol Plant 34, 599–605 (2012). https://doi.org/10.1007/s11738-011-0860-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11738-011-0860-3

Keywords

search

Navigation