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Productivity of greengram in tebuconazole-stressed soil, by using a tolerant and plant growth-promoting Bradyrhizobium sp. MRM6 strain

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Abstract

In this study, a total of 50 rhizobial isolates were recovered from the root nodules of greengram plants. Of the 50 isolates, 9 bradyrhizobial strains namely, MRM1, MRM2, MRM3, MRM4, MRM5, MRM6, MRM7, MRM8, and MRM9, exhibiting a higher tolerance levels of 600, 800, 1,200, 1,000, 1,000, 1,600, 1,400, 1,400, and 1,000 μg ml−1, respectively, to triazole fungicide tebuconazole (chromatographically pure) were selected and tested for plant growth-promoting activities. Generally, the rhizobial strain with maximum fungicide-tolerance ability produced higher amounts of plant growth-promoting substances. Among the nine bacterial strains, Bradyrhizobium strain MRM6 was preferably selected due to its ability to tolerate tebuconazole maximally (up to 1,600 μg ml−1) on minimal salt agar medium. In addition, the strain MRM6 grew well in minimal salts medium supplemented with 100 (recommended), 200 (two times of the recommended), and 300 μg tebuconazole l−1 (three times of the recommended rate) and synthesized highest amounts of plant growth-promoting substances like indole acetic acid, siderophores, exopolysaccharides, hydrogen cyanate, and ammonia, both in the absence and presence of 100, 200, and 300 μg l−1 of tebuconazole. Following these properties, the strain MRM6 was used as inoculant and the inoculated greengram plants was raised in soils treated separately with recommended, two and three times the recommended dose of tebuconazole. Generally, tebuconazole at recommended and the higher rates decreased biomass, nodulation, nutrient-uptake, and grain yield of uninoculated greengram plants. Interestingly, Bradyrhizobium sp. (vigna) strain MRM6 when used with any concentration of tebuconazole, significantly increased the measured phyto-chemical-parameters of greengram plants when compared with those grown in soils treated exclusively (without inoculant) with tebuconazole. This study inferred that the strain MRM6 of Bradyrhizobium sp. (vigna) was compatible with tebuconazole and may be co-inoculated with this fungicide for enhancing the production of legumes especially greengram in soils poisoned with fungicides.

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Abbreviations

CAS:

Chrome Azurol S

DAS:

Days after seeding

IAA:

Indole-3-acetic acid

LB:

Luria Bertani

MTL:

Maximum tolerance level

YEM:

Yeast extract mannitol

References

  • Abd El-Ghany TM, Ahmed AT (2009) Efficacy of certain agrochemicals application at field rates on soil fungi and their ultrastructures. Res J Agric Biol Sci 5:150–160

    CAS  Google Scholar 

  • Ahemad M, Khan MS (2010) Plant growth promoting activities of phosphate solubilizing Enterobacter asburiae as influenced by fungicides. EurAsia J BioSci 4:88–95

    Article  Google Scholar 

  • Ahemad M, Khan MS (2011) Assessment of plant growth promoting activities of rhizobacterium Pseudomonas putida under insecticide stress. Microbiol J 1:54–64

    Article  Google Scholar 

  • Alexander DB, Zuberer DA (1991) Use of chrome azurol S reagents to evaluate siderophore production by rhizosphere bacteria. Biol Fertil Soils 12:39–45

    Article  CAS  Google Scholar 

  • Ayansina ADV (2009) Pesticide use in agriculture and microorganisms. In: Khan MS, Zaidi A, Musarrat J (eds) Microbes in sustainable agriculture. Nova Science Publishers, Inc., New York, pp 261–284

  • Bakker AW, Schipper B (1987) Microbial cyanide production in the rhizosphere in relation to potato yield reduction and Pseudomonas spp mediated plant growth stimulation. Soil Biol Biochem 19:451–457

    Article  CAS  Google Scholar 

  • Becker A, Pühler A (1998) Production of exopolysaccharides. In: Spaink HP, Kondorosi A, Hooykaas PJJ (eds) Rhizobiaceae. Kluwer, Dordrecht, pp 97–118

    Chapter  Google Scholar 

  • Borthakur D, Johnston AWB (1987) Sequence of psi, a gene of the symbiotic plasmid of Rhizobium phaseoli which inhibits exopolysaccharide synthesis and nodulation and demonstration that its transcription is inhibited by psr, another gene on the symbiotic plasmid. Mol Gen Genet 207:149–154

    Article  PubMed  CAS  Google Scholar 

  • Brick JM, Bostock RM, Silversone SE (1991) Rapid in situ assay for indole acetic acid production by bacteria immobilized on nitrocellulose membrane. Appl Environ Microbiol 57:535–538

    Google Scholar 

  • Cernohlavkova J, Jarkovsky J, Hofman J (2009) Effects of fungicides mancozeb and dinocap on carbon and nitrogen mineralization in soils. Ecotoxicol Environ Safety 72:80–85

    Article  PubMed  CAS  Google Scholar 

  • Chen YK, Batley M, Redmond JW, Rolfe BG (1985) Alteration of the effective nodulation properties of a fast growing broad host range Rhizobium due to change in exopolysaccharides synthesis. J Plant Physiol 120:331–349

    CAS  Google Scholar 

  • David LJ, Rick LB, Jack EB, Johnson PD, Brian MR, Virginia LC (2006) Compatibility of in-furrow application of acephate, inoculant and tebuconazole in peanut (Arachis hypogaea L.). Peanut Sci 33:112–117

    Article  Google Scholar 

  • Dubey RC, Maheshwari DK, Kumar H, Choure K (2010) Assessment of diversity and plant growth promoting attributes of rhizobia isolated from Cajanus cajan L. Afr J Biotechnol 9:8619–8629

    CAS  Google Scholar 

  • Dunfield KE, Siciliano SD, Germida JJ (2000) The fungicides thiram and captan affect the phenotypic characteristics of Rhizobium leguminosarum strain C1 as determined by FAME and Biolog analyses. Biol Fertil Soils 31:303–309

    Article  CAS  Google Scholar 

  • Dye DW (1962) The inadequacy of the usual determinative tests for the identification of Xanthomonas spp. Nat Sci 5:393–416

    Google Scholar 

  • Fox JE, Gulledge J, Engelhaupt E, Burow E et al (2007) Pesticides reduce symbiotic efficiency of nitrogen-fixing rhizobia and host plants. PNAS 104:10282–10287

    Article  PubMed  CAS  Google Scholar 

  • Gaind S, Rathi MS, Kaushik BD, Nain L et al (2007) Survival of bio-inoculants on fungicides-treated seeds of wheat, pea and chickpea and subsequent effect on chickpea yield. J Environ Sci Health B 42:663–668

    Article  PubMed  CAS  Google Scholar 

  • Gordon S, Weber RP (1951) The colorimetric estimation of IAA. Plant Physiol 26:192–195

    Article  PubMed  CAS  Google Scholar 

  • Guene NFD, Diouf A, Gueye M (2003) Nodulation and nitrogen fixation of field grown common bean (Phaseolus vulgaris) as influenced by fungicide seed treatment. Afr J Biotechnol 2:198–201

    CAS  Google Scholar 

  • Holt JG, Krieg NR, Sneath PHA, Staley JT et al (1994) Bergey’s manual of determinative bacteriology. Williams and Wilkins, Balitmore

  • Iswaran V, Marwah TS (1980) A modified rapid Kjeldahl method for determination of total nitrogen in agricultural and biological materials. Geobioscience 7:281–282

    Google Scholar 

  • Jackson ML (1967) Soil chemical analysis. Prentice-Hall of India, New Delhi

  • Janczarek M, Skorupska A (2007) The Rhizobium leguminosarum bv. trifolii RosR:transcriptional regulator involved in exopolysaccharide production. Mol Plant Microbes Interact 20:867–881

    Article  CAS  Google Scholar 

  • Kaur C, Maini P, Shukla NP (2007) Effect of captan and carbendazim fungicides on nodulation and biological nitrogen fixation in soybean. Asian J Exp Sci 21:385–388

    Google Scholar 

  • Kishorekumar A, Abdul Jaleel C, Manivannan P, Sankar B, Sridharan R, Panneerselvam R (2007) Comparative effects of different triazole compounds on growth, photosynthetic pigments and carbohydrate metabolism of Solenostemon rotundifolius. Colloids Surf B Biointerfaces 60:207–212

    Article  PubMed  CAS  Google Scholar 

  • Kloepper JW, Leong J, Teintze M, Schroth MN (1980) Enhanced plant growth by siderophores produced by plant growth promoting rhizobacteria. Nature 286:885–886

    Article  CAS  Google Scholar 

  • Kumari BS, Ram MR, Mallaiah KV (2009) Studies on exopolysaccharide and indole acetic acid production by Rhizobium strains from Indigofera. Afr J Microbiol Res 3:10–14

    Google Scholar 

  • Kyei-Boahen S, Slinkard AE, Walley FL (2001) Rhizobial survival and nodulation of chickpea as influenced by fungicide seed treatment. Can J Microbiol 47:585–589

    Article  PubMed  CAS  Google Scholar 

  • Leigh JA, Singer ER, Walker GC (1988) Exopolysaccharide deficient mutants of Rhizobium meliloti that form ineffective nodules. PNAS 82:6231–6235

    Article  Google Scholar 

  • Mohapatra S, Ahuja AK, Deepa M, Jagadish GK, Prakash GS, Kumar S (2010) Behaviour of trifloxystrobin and tebuconazole on grapes under semi-arid tropical climatic conditions. Pest Manag Sci 66:910–915

    PubMed  CAS  Google Scholar 

  • Mody BR, Bindra MO, Modi VV (1989) Extracellular polysaccharides of cowpea rhizobia: compositional and functional studies. Arch Microbiol 1:2–5

    Google Scholar 

  • Morgante JWM, Otieno PE, Cheminingwa GN, Nderitu JH et al (2007) Effect of legume root rot pathogens and fungicide seed treatment on nodulation and biomass accumulation. J Biol Sci 7:1163–1170

    Article  Google Scholar 

  • Perret X, Staehelin C, Broughton W (2000) Molecular basis of symbiotic promiscuity. Microbiol Mol Biol Rev 64:180–201

    Article  PubMed  CAS  Google Scholar 

  • Phipps PM (2003) The response of CBR-susceptible and -resistant cultivars to preplant treatment with metam and in-furrow application of Folicur and Abound for disease management, 2002. F&N Reports 58:FC020

  • Revellin C, Leterme P, Catroux G (1993) Effect of some fungicide treatments on the survival of some Bradyrhizobium japonicum and on the nodulation and yield of soybean (Glycine max L. Merr). Biol Fertil Soils 16:211–214

    Article  CAS  Google Scholar 

  • Reeves MW, Pine L, Neilands JB, Balows A (1983) Absence of siderophore activity in Legionella species grown in iron-deficient media. J Bacteriol 154:324–329

    PubMed  CAS  Google Scholar 

  • Rolfe BG, Carlson RW, Ridge RW, Dazzo RW, Mateos FB, Pankhurst CE (1996) Defective infection and nodulation of clovers by exopolysaccharide mutants of Rhizobium leguminosarum bv. trifolii. Aust J Plant Physiol 23:285–303

    Article  CAS  Google Scholar 

  • Sadasivam S, Manikam A (1992) Biochemical methods for agricultural sciences. Wiley Eastern Limited, New Delhi

  • Shew BB (2006) Peanut disease management. In: 2006 Peanut Information. North Carolina Coop. Ext. Ser. AG-331, pp 79–104, 116

  • Singh N, Dureja P (2009) Effect of biocompost-amendment on degradation of triazoles fungicides in soil. Bull Environ Contam Toxicol 82:120–123

    Article  PubMed  CAS  Google Scholar 

  • Somasegaran P, Hoben HJ (1994) Handbook for Rhizobia: methods in legume Rhizobium technology. Springer, Berlin

  • Spaink HP (2000) Root nodulation and infection factors produced by rhizobial bacteria. Annu Rev Microbiol 54:257–288

    Article  PubMed  CAS  Google Scholar 

  • Tomlin CDS (1997) The pesticide manual. The British Crop Protection Council, Surrey

    Google Scholar 

  • van Workum WA, Canter Cremers HCJ, Wijfjes AHM, van der Kolk C, Wijffelman CA, Kijne JW (1997) Cloning and characterization of four genes of Rhizobium leguminosarum bv. trifolii involved in exopolysaccharide production and nodulation. Mol Plant Microbe Interact 10:290–301

    Article  PubMed  Google Scholar 

  • Vincent JM (1970) A manual for the practical study of root nodule bacteria. Blackwell Scientific Publications, Oxford

  • Wani PA, Khan MS, Zaidi A (2008) Chromium-reducing and plant growth-promoting Mesorhizobium improves chickpea growth in chromium-amended soil. Biotechnol Lett 30:159–163

    Article  PubMed  CAS  Google Scholar 

  • Yang C, Lee C (2008) Enrichment, isolation, and characterization of 4-chlorophenol-degrading bacterium Rhizobium sp. 4-CP-20. Biodegradation 19:329–336

    Article  PubMed  CAS  Google Scholar 

  • Zablotowicz RM, Reddy KN (2004) Impact of glyphosate on the Bradyrhizobium japonicum symbiosis with glyphosate-resistant transgenic soybean: a minireview. J Environ Qual 33:825–831

    Article  PubMed  CAS  Google Scholar 

  • Zahran HH (1999) Rhizobium-legume symbiosis and nitrogen fixation under severe conditions and in an arid climate. Microbiol Mol Biol Rev 63:968–989

    PubMed  CAS  Google Scholar 

Download references

Acknowledgments

The authors thank Dr. N. A. Naqvi, Parijat Agrochemicals, New Delhi, India, for providing technical grade fungicides. Financial assistance from University Grants Commission (UGC), New Delhi, India is also gratefully acknowledged.

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The authors have declared no conflict of interest.

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Authors and Affiliations

  1. Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh, 202002, UP, India

    Munees Ahemad & Mohammad Saghir Khan

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  1. Munees Ahemad
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  2. Mohammad Saghir Khan
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Correspondence to Mohammad Saghir Khan.

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Communicated by B. Barna.

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Ahemad, M., Khan, M.S. Productivity of greengram in tebuconazole-stressed soil, by using a tolerant and plant growth-promoting Bradyrhizobium sp. MRM6 strain. Acta Physiol Plant 34, 245–254 (2012). https://doi.org/10.1007/s11738-011-0823-8

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  • DOI: https://doi.org/10.1007/s11738-011-0823-8

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