Advertisement

Acta Physiologiae Plantarum

, 33:2501 | Cite as

A rapid in vitro propagation of red sanders (Pterocarpus santalinus L.) using shoot tip explants

  • K. BalarajuEmail author
  • P. Agastian
  • S. Ignacimuthu
  • Kyungseok Park
Original Paper

Abstract

An efficient protocol has been developed for the in vitro propagation of Pterocarpus santalinus L. using shoot tip explants which is a valuable woody medicinal plant. Various parts of this plant are pharmaceutically used for the treatment of different diseases. Multiple shoots were induced from shoot tip explants derived from 20 days old in vivo germinated seedlings on 1:1 ratio of sand and soil after treating with gibberellic acid (GA3). The highest frequency for shoot regeneration (83.3%) with maximum number of shoot buds (11) per explant was obtained on Murashige and Skoog (MS) medium supplemented with 1.0 mg/l of 6-benzylaminopurine (BAP) along with 0.1 mg/l of thidiazuron (TDZ) after 45 days of culture. A proliferating shoot culture was established by repeatedly subculturing the original shoot tip explants on fresh medium after each harvest of the newly formed shoots. Sixty percent of the shoots produced roots were transferred to rooting medium containing MS salts and 0.1 mg/l indole-3-butyric acid (IBA) after 30 days. About 73.33% of the in vitro raised plantlets were established successfully in earthen pots. Random amplified polymorphic DNA (RAPD)-based DNA fingerprinting profiles were generated for the first time using shoot tip explants of this species and confirmed that there was no genetic variability. This protocol might be helpful for the mass multiplication of P. santalinus in the future.

Keywords

Apical meristem RAPD fingerprinting MS medium Multiple shoots Pterocarpus santalinus 

Abbreviations

BAP

6-benzylaminopurine

GA3

Gibberellic acid

IAA

Indole-3-acetic acid

IBA

Indole-3-butyric acid

KN

Kinetin

MS

Murashige and Skoog

NAA

α-Naphthalene acetic acid

TDZ

Thidiazuron

Notes

Acknowledgments

The authors are grateful to the University Grants Commission, New Delhi, India, for providing financial assistance (Sanction No. F.31-238/2005 (SR) dated 05.04.2006).

References

  1. Anis M, Husain MK, Shahzad A (2005) In vitro plantlet regeneration of Pterocarpus marsupium Roxb. an endangered leguminous tree. Curr Sci 88:861–863Google Scholar
  2. Anuradha M, Pullaiah T (1999) In vitro seed culture and induction of enhanced axillary branching in Pterocarpus santalinus and a method for rapid multiplication. Phyotomorphology 49:157–163Google Scholar
  3. Arockiasamy S, Ignacimuthu S, Melchias G (2000) Influence of growth regulators and explant type on in vitro shoot propagation and rooting of red sandalwood (Pterocarpus santalinus L.). Ind J Exp Bot 38:1270–1273Google Scholar
  4. Asthana P, Jaiswal VS, Jaiswal U (2011) Micropropagation of Sapindus trifoliatus L. and assessment of genetic fidelity of micropropagated plants using RAPD analysis. Acta Physiol Plant. doi: 10.1007/s11738-011-0721-0
  5. Berger K, Schaffner W (1995) In vitro propagation of the leguminous tree Swartzia madegascariensis. Plant Cell Tissue Organ Cult 40:289–291CrossRefGoogle Scholar
  6. Bisht MS, Vyas P, Bag N, Palni LMS (1998) Micropropagation of some plants of Indian Himalayan region. In plant tissue culture and molecular biology—applications and prospects. Narosa Publishing House, New Delhi, pp 126–170Google Scholar
  7. Caboni ED, Angeli S, Chiappetta A, Innocenti AM, Van OH, Damiano C (2002) Adventitious shoot regeneration from vegetative shoot apices in pear and putative role of cytokinin accumulation in the morphogenetic process. Plant Cell Tissue Organ Cult 70:199–206CrossRefGoogle Scholar
  8. Ceasar SA, Maxwell SL, Prasad KB, Karthigan M, Ignacimuthu S (2010) Highly efficient shoot regeneration of Bacopa monnieri (L.) using a two-stage culture procedure and assessment of genetic integrity of micropropagated plants by RAPD. Acta Physiol Plant 32:443–452CrossRefGoogle Scholar
  9. Chand S, Singh AK (2004a) In vitro shoot regeneration from cotyledonary node explants of a multipurpose leguminous tree, Pterocarpus marsupium Roxb. In Vitro Cell Dev Biol 40:167–170CrossRefGoogle Scholar
  10. Chand S, Singh AK (2004b) In vitro shoot regeneration from cotyledonary node explants of a multipurpose leguminous tree, Pterocarpus marsupium Roxb. In Vitro Cell Dev Biol 40:464–466CrossRefGoogle Scholar
  11. Chaturani GDG, Subasinghe S, Jayatilleke MP (2006) In vitro establishment, germination and growth performance of Red sandal wood (Pterocarpus santalinus L.). Trop Agric Res Ext 9:116–130Google Scholar
  12. Cho JY, Park J, Kim PS, Yoo ES, Baik KU, Park MH (2001) Savinin a lignan from Pterocarpus santalinus inhibits tumor necrosis factor-alpha production and T-cell proliferation. Biol Pharm Bull 71:10–13Google Scholar
  13. Das M, Pal A (2005) Clonal propagation and production of genetically uniform regenerants from axillary meristems of adult bamboo. J Plant Biochem Biotechnol 14:185–188Google Scholar
  14. Dayanand T, Lohidas T (1998) Effect of different treatments on pod germination of red sanders (Pterocarpus santalinus L.). Indian J Forest 11:87–88Google Scholar
  15. Dewan A, Nanda K, Gupta SC (1992) In vitro micropropagation of Acacia nilotica subsp. Indica Brenen via cotyledonary nodes. Plant Cell Rep 12:18–21CrossRefGoogle Scholar
  16. Elghag HM (1991) In vitro propagation of Catha edulis. Hortic Sci 26:212Google Scholar
  17. Feyissa T, Welander M, Negash L (2005) Micropropagation of Hagenia abyssinica (Bruce) J.F. Gmel: a multipurpose tree. Plant Cell Tissue Organ Cult 80:119–127CrossRefGoogle Scholar
  18. Husain MK, Anis M, Shahzad A (2007) In vitro propagation of Indian Kino (Pterocarpus marsupium Roxb.) using thidiazuron. In Vitro Cell Dev Biol Plant 43:59–64Google Scholar
  19. Husain MK, Anis M, Shahzad A (2008) In vitro propagation of a multipurpose leguminous tree (Pterocarpus marsupium Roxb.) using nodal explants. Acta Physiol Plant 30:353–359CrossRefGoogle Scholar
  20. Jha AK, Prakash S, Jain N, Nanda K, Gupta SC (2004) Micropropagation of Sesbania rostrata from the cotyledonary node. Biol Plant 48:289–292CrossRefGoogle Scholar
  21. Kalimuthu K, Lakshmanan KK (1995) Effect of different treatments on pod germination of Pterocarpus species. Indian J For 18:104–106Google Scholar
  22. Kameswara Rao B, Giri R, Kesavulu MM, Apparao C (2001) Effect pf pra; administration of bark extracts of Pterocarpus santalinus on blood glucose level in experimental animals. J Ethnopharmacol 74:69–74PubMedCrossRefGoogle Scholar
  23. Kaur K, Verma B, Kant U (1998) Plants obtained from the Khair tree (Acacia catechu Wild) using mature nodal segment. Plant Cell Rep 17:427–429CrossRefGoogle Scholar
  24. Kesava Reddy K, Srivasuki KP (1990) Vegetative propagation of red sanders (Pterocarpus santalinus Linn.). Indian Forest 116:536–540Google Scholar
  25. Koroch K, Juliani HP, Kapteyn J, Simon JE (2003) In vitro regeneration of Echinacea purpurea from leaf explants. Plant Cell Tissue Organ Cult 69:79–83CrossRefGoogle Scholar
  26. Luis PBC, Adraine CMGM, Silvica BRCC, Anna Christina MB (1999) Plant regeneration from seedling explants of Eucalyptus grandis x E. urophylla. Plant Cell Tiss Org Cult 56:17–23CrossRefGoogle Scholar
  27. Manjunatha BK (2006) Hepatoprotective activity of Pterocarpus santalinus L.f., an endangered medicinal plant. Indian J Pharmacol 38:25–28CrossRefGoogle Scholar
  28. Murashige T, Skoog FA (1962) Revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497CrossRefGoogle Scholar
  29. Naidu CV, Rajendrudu G (2001) Influence of kinetin and nitrogenous salts on seed germination of red sanders (Pterocarpus santalinus Linn.F.). Seed Sci Technol 29:669–672Google Scholar
  30. Onay A (2000) Micropropagation of Pistachio from mature trees. Plant Cell Tiss Org Cult 60:159–162CrossRefGoogle Scholar
  31. Pereira AMS, Moro JR, Cedeira RMM, Franca SC (1995) Effect of phytoregulators and physiological characteristics of the explants on micropropagation of Maytenus ilicifolia. Plant Cell Tissue Organ Cult 42:295–297CrossRefGoogle Scholar
  32. Perinet P, Lalonde K (1983) In vitro propagation and nodulation of actinorhizal host plant Alnus glutinosa (L.) Gaertn. Plant Sci Lett 29:9–17CrossRefGoogle Scholar
  33. Pradhan C, Kar S, Pattnaik S, Chand PK (1998) Propagation of Dalbergia sissoo Roxb. through in vitro shoot proliferation from cotyledonary nodes. Plant Cell Rep 18:122–126CrossRefGoogle Scholar
  34. Prakash E, Khan PSSV, Sreenivasa Rao TJV, Meru ES (2006) Micropropagation of red sanders (Pterocarpus santalinus L.) using mature nodal explants. J For Res 11:329–335CrossRefGoogle Scholar
  35. Purohit SD, Dave A (1996) Micropropagation of Sterculia urens Roxb, an endangered tree species. Plant Cell Rep 15:704–706CrossRefGoogle Scholar
  36. Purohit VK, Palni LMS, Nandi SK, Rikhari HC (2002a) In vitro plant regeneration through cotyledonary nodes of Quercus floribunda Lindl. ex A. Camus (Tilonj oak), a high value tree species of central Himalaya. Curr Sci 833:101–104Google Scholar
  37. Purohit VK, Tamta S, Chandra S, Vyas P, Palni LMS, Nandi SK (2002b) In vitro multiplication of Quercus leucotrichophora and Q. glauca: important Himalayan oaks. Plant Cell Tiss Org Cult 69:121–133CrossRefGoogle Scholar
  38. Rajeswari V, Paliwal K (2008) In vitro plant regeneration of red sanders (Pterocarpus santalinus L.f.) from cotyledonary nodes. Indian J Biotechnology 7:541–546Google Scholar
  39. Raju KK, Nagaraju A (1999) Geobotany of red sanders (Pterocarpus santalinus)—a case study from the South-eastern portion of Andhra Pardesh. Environ Geol 37:340–344CrossRefGoogle Scholar
  40. Romano A, Barros S, Martinus-Lucao MA (2002) Micropropagation of the Mediterranean tree Ceratomia siliqua. Plant Cell Tiss Org Cult 68:35–41CrossRefGoogle Scholar
  41. Sanjappa M (2001) Leguminosae. In: Singh NP, Singh DK (eds) Floristic diversity and conservation strategies in India, vol 4. Botanical Survey of India, Dehra Dun, pp 1846–1960Google Scholar
  42. Sha Valli Khan PS, Prakash E, Rao KR (1997) In vitro micropropagation of an endemic fruit tree of Syzygium alternifolium (Wight.) Walp. Plant Cell Rep 16:325–328CrossRefGoogle Scholar
  43. Shailendra V, Joshi N, Tak K, Purohit SD (2005) In vitro adventitious shoot bud differentiation and plantlet regeneration in Feronia limonia L. (swingle). In Vitro Cell Dev Biol Plant 41:296–302CrossRefGoogle Scholar
  44. Sharma AK, Prasad RN, Chaturvedi HC (1981) Clonal propagation of Bougainvillea glabra ‘Magnifica’ through shoot apex culture. Plant Cell Tiss Organ Cult 1:33–38CrossRefGoogle Scholar
  45. Shekhawat NS, Rathore TS, Singh RP, Deora NS, Rao SR (1993) Factors affecting in vitro clonal propagation of Prosopis cineraria. Plant Growth Regul 12:273–280CrossRefGoogle Scholar
  46. Sita GL, Sreenatha KS, Sujatha S (1992) Plantlet production from shoot tip cultures of Red sandalwood (Pterocarpus santalinus L.). Curr Sci 62:7–10Google Scholar
  47. Suresh C, Ajay KS (2004) In vitro shoot regeneration from cotyledonary node explants of a multipurpose leguminous tree, Pterocarpus marsupium Roxb. In Vitro Cell Dev Biol Plant 40:167–170CrossRefGoogle Scholar
  48. Tiwari SK, Kashyap MK, Ujjaini MM, Agrawal AP (2002) In vitro propagation of Lagerstromia parviflora Roxb. from adult tree. Indian J Exp Biol 40:212–215PubMedGoogle Scholar
  49. Tiwari S, Shah P, Singh K (2004) In vitro propagation of Pterocarpus marsupium Roxb.: an endangered medicinal tree. Indian J Biotechnol 3:422–425Google Scholar
  50. Trigiano RN, Geneve RL, Merkle SA, Preece JE (1992) Tissue and cell cultures of woody legumes. Hortic Rev 14:265–332Google Scholar

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2011

Authors and Affiliations

  • K. Balaraju
    • 2
    • 3
    Email author
  • P. Agastian
    • 1
  • S. Ignacimuthu
    • 2
  • Kyungseok Park
    • 3
  1. 1.Department of Plant Biology and Biotechnology, School of Life SciencesLoyola CollegeChennaiIndia
  2. 2.Plant Biotechnology Division, Entomology Research InstituteLoyola CollegeChennaiIndia
  3. 3.National Academy of Agricultural Science (NAAS)RDASuwonSouth Korea

Personalised recommendations