Acta Physiologiae Plantarum

, 33:2501 | Cite as

A rapid in vitro propagation of red sanders (Pterocarpus santalinus L.) using shoot tip explants

  • K. BalarajuEmail author
  • P. Agastian
  • S. Ignacimuthu
  • Kyungseok Park
Original Paper


An efficient protocol has been developed for the in vitro propagation of Pterocarpus santalinus L. using shoot tip explants which is a valuable woody medicinal plant. Various parts of this plant are pharmaceutically used for the treatment of different diseases. Multiple shoots were induced from shoot tip explants derived from 20 days old in vivo germinated seedlings on 1:1 ratio of sand and soil after treating with gibberellic acid (GA3). The highest frequency for shoot regeneration (83.3%) with maximum number of shoot buds (11) per explant was obtained on Murashige and Skoog (MS) medium supplemented with 1.0 mg/l of 6-benzylaminopurine (BAP) along with 0.1 mg/l of thidiazuron (TDZ) after 45 days of culture. A proliferating shoot culture was established by repeatedly subculturing the original shoot tip explants on fresh medium after each harvest of the newly formed shoots. Sixty percent of the shoots produced roots were transferred to rooting medium containing MS salts and 0.1 mg/l indole-3-butyric acid (IBA) after 30 days. About 73.33% of the in vitro raised plantlets were established successfully in earthen pots. Random amplified polymorphic DNA (RAPD)-based DNA fingerprinting profiles were generated for the first time using shoot tip explants of this species and confirmed that there was no genetic variability. This protocol might be helpful for the mass multiplication of P. santalinus in the future.


Apical meristem RAPD fingerprinting MS medium Multiple shoots Pterocarpus santalinus 





Gibberellic acid


Indole-3-acetic acid


Indole-3-butyric acid




Murashige and Skoog


α-Naphthalene acetic acid





The authors are grateful to the University Grants Commission, New Delhi, India, for providing financial assistance (Sanction No. F.31-238/2005 (SR) dated 05.04.2006).


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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2011

Authors and Affiliations

  • K. Balaraju
    • 2
    • 3
    Email author
  • P. Agastian
    • 1
  • S. Ignacimuthu
    • 2
  • Kyungseok Park
    • 3
  1. 1.Department of Plant Biology and Biotechnology, School of Life SciencesLoyola CollegeChennaiIndia
  2. 2.Plant Biotechnology Division, Entomology Research InstituteLoyola CollegeChennaiIndia
  3. 3.National Academy of Agricultural Science (NAAS)RDASuwonSouth Korea

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