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Acta Physiologiae Plantarum

, Volume 33, Issue 4, pp 1547–1551 | Cite as

Development of an optimal culture system for callogenesis of Chrysanthemum indicum protoplasts

  • Tom EeckhautEmail author
  • Johan Van Huylenbroeck
Short Communication

Abstract

This study is a comparison of four methods to induce calli formation in a protoplast culture of Chrysanthemum indicum. Culture in liquid medium (17.6 calli/105 protoplasts) was preferable to culture in solid agarose beads, although the efficiency of the latter could be improved by layering them on glass beads (12.5 vs. 0.83 calli/105 protoplasts). Culture of protoplasts on moistened filter paper was unsuccessful. In the liquid media, microcalli and calli were induced efficiently and easily after 6 weeks. These effects may be explained by reduced toxicity due to cell breakdown and improved aeration.

Keywords

Aeration In vitro Liquid medium Microcalli Regeneration 

Notes

Acknowledgments

The authors wish to thank Ronald Van Den Oord for his technical assistance.

References

  1. Amagasa K, Kameya T (1989) Plant regeneration and callus formation from Chrysanthemum morifolium, C. coronarium and Lactuca sativa protoplasts. J Japan Soc Hort Sci 57:620–625Google Scholar
  2. Anthony P, Davey M, Power J, Lowe K (1995) An improved protocol for the culture of cassava leaf protoplasts. Plant Cell Tissue Organ Cult 42:299–302CrossRefGoogle Scholar
  3. Davey M, Anthony P, Power J, Lowe K (2005) Plant protoplasts: status and biotechnological perspectives. Biotechnol Adv 23:131–171PubMedCrossRefGoogle Scholar
  4. Eeckhaut T, Van Laere K, De Riek J, Van Huylenbroeck J (2006) Overcoming interspecific barriers in ornamental plant breeding. In: Teixeira da Silva J (ed) Floriculture, ornamental and plant biotechnology: advances and topical issues, 1st edn. Global Science Books, London, pp 540–551Google Scholar
  5. Endo M, Fujii N, Fujita S, Inada I (1997) Improvement of plating efficiency on the mesophyll protoplast culture of Chrysanthemum, dendranthema × grandiflorum (Ram.) Kitam. Plant Biotechnol 14:81–83Google Scholar
  6. Fujii Y, Shimizu K (1990) Callus formation from mesophyll protoplasts of pyrethrum (Chrysanthemum coccineum). Plant Tissue Cult Lett 7:111–113Google Scholar
  7. Furuta H, Shinoyama H, Nomura Y, Maeda M, Makara K (2004) Production of intergeneric somatic hybrids of chrysanthemum [Dendranthema × grandiflorum (Ramat.) Kitamura] and wormwood (Artemisia sieversiana J.F.Ehrh. ex. Willd) with rust (Puccinia horiana Henning) resistance by electrofusion of protoplasts. Plant Sci 166:695–702CrossRefGoogle Scholar
  8. Kao K, Michayluk M (1975) Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126:105–110CrossRefGoogle Scholar
  9. Lindsay G, Ledger S (1993) A protoplast to plant system for the chrysanthemum Dendranthema zawadskii × D. grandiflora. Plant Cell Rep 12:278–280CrossRefGoogle Scholar
  10. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  11. Okamura M, Hayashi T, Miyazaki S (1984) Inhibiting effect of ammonium ion in protoplast culture of some Asteraceae plants. Plant Cell Physiol 25:281–286Google Scholar
  12. Sauvadet M, Brochard P, Boccon-Gibod J (1990) A protoplast-to-plant system in chrysanthemum: differential responses among several commercial clones. Plant Cell Rep 8:692–695CrossRefGoogle Scholar
  13. Teixeira Da Silva J (2003) Chrysanthemum: advances in tissue culture, cryopreservation, postharvest technology, genetics and transgenic biotechnology. Biotechnol Adv 21:715–766PubMedCrossRefGoogle Scholar
  14. Teixeira Da Silva J (2004) Ornamental chrysanthemums: improvement by biotechnology. Plant Cell Tissue Organ Cult 79:1–18CrossRefGoogle Scholar
  15. Waara S, Glimelius K (1995) The potential of somatic hybridization in crop breeding. Euphytica 85:217–233CrossRefGoogle Scholar
  16. Zhou J, Wang B, Zhu L (2005) Conditioned culture for protoplasts isolated from chrysanthemum: an efficient approach. Colloids Surf B Biointerfaces 45:113–119PubMedCrossRefGoogle Scholar

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2010

Authors and Affiliations

  1. 1.Institute for Agricultural and Fisheries Research (ILVO), Plant Sciences Unit-Applied Genetics and BreedingMelleBelgium

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