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Exogenous nitric oxide protects against salt-induced oxidative stress in the leaves from two genotypes of tomato (Lycopersicom esculentum Mill.)

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Abstract

Nitric oxide (NO) has emerged as a key molecule involved in many physiological events in plants. To characterize roles of NO in tolerance of tomato (Lycopersicom esculentum Mill.) to salt stress, the protective effects of NO against salt-induced oxidative stress in the leaves of tomato cultivar Hufan1480 (salt-tolerant) and Hufan2496 (salt-sensitive) were evaluated. Under salt stress, Hufan1480 showed higher biomass accumulation, and less oxidative damage when compared with the Hufan2496. Application of exogenous sodium nitroprusside, a NO donor, dramatically alleviated growth suppression induced by salt stress in two tomato ecotypes, reflected by decreased malondialdehyde and O ·−2 production. Furthermore, the antioxidant enzymes superoxide dismutase, guaiacol peroxidase, catalase and ascorbate peroxidase, the antioxidant metabolites ascorbate and reduced glutathione, and the osmosis molecules proline and soluble sugar were increased in both ecotypes in the presence of NO under salt stress. Therefore, the protective effect of NO against salt-induced oxidative damages in tomato seedlings is most likely mediated through stimulation of antioxidant system.

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Acknowledgments

This work was supported by the Shanghai Committee of Science and Technology, China (Grant No. 09JC1412700) and by Shanghai Prospering Agriculture by Science and Technology Plan, China (Grant No. 2006-2-1).

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Authors and Affiliations

  1. Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, 201106, Shanghai, China

    Xuexia Wu, Weimin Zhu, Hui Zhang & Haidong Ding

  2. Shanghai Key Lab of Protected Horticultural Technology, 201106, Shanghai, China

    Weimin Zhu

  3. College of Biosciences and Biotechnology, Yangzhou University, 225009, Yangzhou, China

    Hong Juan Zhang

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  1. Xuexia Wu
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Correspondence to Haidong Ding.

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Communicated by G. Bartosz.

X. Wu, W. Zhu contributed equally to this work.

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Wu, X., Zhu, W., Zhang, H. et al. Exogenous nitric oxide protects against salt-induced oxidative stress in the leaves from two genotypes of tomato (Lycopersicom esculentum Mill.). Acta Physiol Plant 33, 1199–1209 (2011). https://doi.org/10.1007/s11738-010-0648-x

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