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Fluoranthene-induced production of ethylene and formation of lysigenous intercellular spaces in pea plants cultivated in vitro

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Abstract

The effect of increasing concentration of polycyclic aromatic hydrocarbon (PAH) fluoranthene (FLT; 0.1, 1 and 5 mg l−1) on the growth, ethylene production and anatomy of stems of 21-day-old pea plants cultivated in vitro in MS medium, with or without FLT, enriched with 0.1 mg l−1 indole-3-acetic acid (IAA) or with combination of 0.1 mg l−1 IAA + 0.1 mg l−1 N6-benzyladenine (BA) were investigated. The low concentration of 0.1 mg l−1 FLT, in both IAA- and IAA + BA-treated plants, significantly stimulated the growth of pea callus, while higher concentrations 1 mg l−1 and especially 5 mg l−1 FLT significantly inhibited it. Pea shoots were significantly influenced only after application of 5 mg l−1 FLT in IAA treatment. Significantly increased production of ethylene was found in IAA + BA treatments in all concentrations of FLT, whereas in IAA treatments in 1 and 5 mg l−1 FLT. The lysigenous aerenchyma formation in the cortex of pea stems significantly increased in all FLT treatments and its highest proportion was found in plants exposed to 1 mg l−1 FLT.

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Acknowledgments

This work was supported by Czech Science Foundation (GAČR 522/09/0239).

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Authors and Affiliations

  1. Department of Plant Physiology and Anatomy, Institute of Experimental Biology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37, Brno, Czech Republic

    Lucie Váňová & Marie Kummerová

  2. Department of Plant Physiology, Faculty of Science, Charles University in Prague, Viničná 5, 128 44, Prague, Czech Republic

    Olga Votrubová

Authors
  1. Lucie Váňová
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  2. Marie Kummerová
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  3. Olga Votrubová
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Correspondence to Lucie Váňová.

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Communicated by J. Ueda.

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Váňová, L., Kummerová, M. & Votrubová, O. Fluoranthene-induced production of ethylene and formation of lysigenous intercellular spaces in pea plants cultivated in vitro. Acta Physiol Plant 33, 1037–1042 (2011). https://doi.org/10.1007/s11738-010-0618-3

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  • DOI: https://doi.org/10.1007/s11738-010-0618-3

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