Abstract
The present study was aimed at improving sprouting and establishment of bud chip seed stocks of sugarcane cultivar CoSe 92423 by pre-planting soaking in growth-promoting chemicals viz ethephon (0.1 g dm−3) and calcium chloride (1 g dm−3) along with water-soaked control for 24 h. Treated bud chips recorded higher bud sprouting, shoot height, root number, fresh weight of leaves, shoot and roots, and plant vigor index. In both the treatments, reducing sugars contents, acid invertase, and ATPase activity increased in developing sprouts; increase was about 86.5 and 40.7% in reducing sugars, 28 and 70% in acid invertase, and 15 and 23% in ATPase activities over control by ethephon and calcium chloride treatment, respectively. Reducing sugars contents and activity of acid invertase and ATPase enzymes of sprouted buds exhibited significant positive correlation with bud sprouting and plant vigor index. These findings indicate that soaking of bud chips in growth-promoting chemicals viz ethephon (0.1 g dm−3) and calcium chloride (1 g dm−3) solutions helps in enhancing bud sprouting, root growth, and plant vigor by altering some of the key biochemical attributes essential for the early growth and better establishment of bud chips under field conditions which is otherwise poor in untreated chips.
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Abbreviations
- ATPase:
-
Adenosine triphosphatase
- EDTA:
-
Ethylenediaminetetraacetic acid
- HCl:
-
Hydrochloric acid
- TCA:
-
Trichloroacetic acid
- KCl:
-
Potassium chloride
- FW:
-
Fresh weight
References
Anthony BB, Schaller GE (1996) The mechanism of ethylene perception. Plant Physiol 111:653–660
Bewly JD, Black BM (1982) Germination of seeds. In: Khan AA (ed) Physiology and biochemistry of seed germination. Springer, New York, pp 40–80
Burstrom HG (1968) Calcium and plant growth. Biol Rev 43:287–316
Cassab GI, Iin JJ, Lin LS, Varner JE (1988) Ethylene effect on extension and peroxidase distribution in the sub-apical region of pea epicotyls. Plant Physiol 88:522–524
Chrispeels MJ, Varner JE (1967) Gibberellic acid enhanced synthesis and release of amylase and ribonuclease by isolated barley aleurone layers. Plant Physiol 42:398–406
Davidson FM, Long C (1958) The structure of the naturally occurring phosphoglycerides. 4. Action of cabbage-leaf phospholipase D on ovolecithin and related substances. Biochem J 69:458–466
Fisher J, Hodges TK (1969) Monovalent ion stimulated adenosine triphosphatase from oat roots. Plant Physiol 44:385–395
Fiske CH, Subbarow YJ (1925) The colorimetric determination of phosphorus. J Biol Chem 66:375–400
Gayler KR, Glasziou KT (1972) Physiological functions of acid and neutral invertases in growth and sugar storage in sugarcane. Physiol Plant 27:25–31
Gokhale MN (1977) A new approach to cane seed nurseries. Sugar technologists Association of India, sixth joint convention, pp 163–166
Hatch MD, Glasziou KT (1963) I. Sugar accumulation cycle in sugarcane. II. Relationship of invertase activity to sugar content and growth rate in storage tissue of plants grown in controlled environments. Plant Physiol 38:344–348
Hepler PK (2005) Calcium: a central regulator of plant growth and development. Plant Cell 17:2142–2155
Hepler PK, Wayne RO (1985) Calcium and plant development. Annu Rev Plant Physiol 36:379–439
Iqbal MT, Eusufzal SUK, Rukshana F (2002) Performance of sugarcane bud chip settlings. Indian J Sugarcane Technol 17:88
Jain R, Shrivastava AK, Solomon S, Yadav RL (2007) Low temperature stress-induced biochemical changes in stubble bud sprouting in sugarcane (Saccharum sp. hybrid). Plant Growth Regul 53:17–23
Jain R, Solomon S, Lal P, Shrivastava AK (2009) Nutrient application improves stubble bud sprouting under low temperature conditions in sugarcane. Sugar Tech 11(1):83–85
Kalckar HM (1944) Adenylpyrophosphatase and myokinase. J Biol Chem 153:355–367
Leopold AC, Poovaiah BW, DeLa Fuente RK, Williams RJ (1974) Regulation of growth with inorganic solutes. In: Sumiki Y (ed) Plant growth substances. Hirokawa, Tokyo, pp 780–788
Li YR, Solomon S (2003) Ethephon, a versatile growth regulator for sugarcane industry. Sugar Tech 5:213–224
Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–375
Marschner H (1995) Mineral nutrition of higher plants, 2nd edn. Academic Press, London
Narasimha Rao G (1977) Chip bud method. The Hindu, July 9, 1977
Narasimha Rao G, Satyanarayana Y (1974) Studies in control of sett borne infection of red rot of sugarcane. J Res Andhra Pradesh Agric Univ 1:83–86
Narendranath M (1992) Cost-effectiveness of transplanting nursery-raised sugarcane bud chip plants on commercial sugar cane plantations. In: Proceedings of 11th International Society for Sugar Cane Technologists, Poster paper, p 332
Nelson N (1944) A photometric adaptation of the Somogy’s method for determination of glucose. J Biol Chem 153:375–380
Panse VG, Sukhatme P (1985) Statistical methods for agricultural workers, 4th edn. ICAR, New Delhi
Prasad RN, Sreenivasan TV (1996) Developing technology for sugarcane varietal exchange through bud chips. Indian J Sugarcane Technol 11:25–28
Ramaiah BB, Narasimha Rao G, Prasad GH (1977) Elimination of internodes in sugarcane seed piece. In: Proceedings of International Society for Sugar Cane Technologists, pp 1509–1513
Raven PH, Evert RF, Eichhorn SE (1992) Biology of plants. Worth, New York, pp 545–572
Reddy TB, Reddy TY, Reddy DS, Murthy CK, Rao MRM, Reddy GHS (1986) Effect of different types of seed material on growth and yield of sugarcane. Coop Sugar 17:947–949
Shrivastava AK, Solomon S, Singh P, Rai RK, Jain R, Singh I, Kumar R (2006) Improving sprouting of stubble buds in sugarcane under sub-optimal temperatures by pre-harvest foliar application of ethrel. In: Li YR, Solomon S (eds) Technologies to improve sugar productivity in developing countries. China Agricultural Press, Beijing, pp 495–499
Solomon S, Kumar A (1987) Status of β-fructofuranosidase during germination of sugarcane setts. Indian J Sugarcane Tech 4:11–16
Solomon S, Singh I, Madan VK (1998) Effect of 2-chloroethyl phosphonic acid on early growth and advancement of maturity in sugarcane. In: Proceedings of 60th annual convention of the Sugar Technologists Association of India, Shimla
Solomon S, Shahi HN, Duttamajumder SK, Singh I, Madan VK (2001) Effect of ethephon on sugarcane grown under subtropical climate. In: Proceedings of International Society for Sugar Cane Technologists, vol 24, pp 177–179
Tamilselvan N (2006) Sugar cane response to chip bud method of planting. In: Proceedings of International Society for Sugar Cane Technologists, Agronomy Workshop, Khon Kaen, Thailand, 23–26 May 2006
Van Andel OM (1973) Morphological effects on vegetative plants of Poa pratensis L. of 6-azuuacil, 2-chloroethylphosphonic acid and 2-chloroethyl trimethyl ammonium chloride and their interaction with gibberellic acid. J Exp Bot 24:245–247
van Dillewijn C (1952) The Chronica Botanica Co., USA
White PJ, Broadley MR (2003) Calcium in plants. Ann Bot 92:1–25
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We would like to thank Director, Indian Institute of Sugarcane Research, Lucknow, India for his encouragement and critical reading of this manuscript.
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Jain, R., Solomon, S., Shrivastava, A.K. et al. Effect of ethephon and calcium chloride on growth and biochemical attributes of sugarcane bud chips. Acta Physiol Plant 33, 905–910 (2011). https://doi.org/10.1007/s11738-010-0617-4
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DOI: https://doi.org/10.1007/s11738-010-0617-4