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Combination effect of lactoalbumin hydrolysate and methyl jasmonate on ginsenoside and polysaccharide production in Panax quinquefolium L. cells cultures

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Abstract

Lactoalbumin hydrolysate (LH) at 100 mg L−1 with methyl jasmonate (MJ) at 2 mg L−1 synergistically stimulated ginsenoside accumulation in Panax quinquefolium cells compared with 100 mg L−1 LH. Combination elicitors led to higher ginsenoside productivity (45.93 mg L−1) than single treatment of 100 mg L−1 LH (31.37 mg L−1). This present result will be helpful in providing a tool for enhancing the productivity of ginsenoside by Panax quinquefolium cell cultures on a commercial scale.

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Abbreviations

MS:

Murashige and Skoog

2,4-D:

2,4-Dichlorophenoxyacetic acid

KT:

Kinetin

LH:

Lactoalbumin hydrolysate

MJ:

Methyl jasmonate

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Acknowledgments

This research was funded by China National Center for Biotechnology Development (2006BAI06A16-02).

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Authors and Affiliations

  1. School of Pharmaceutical Science and Technology, Tianjin University, No. 92 Weijin Road, Nankai District, Tianjin, 300072, People’s Republic of China

    Juan Wang, Wen-Yuan Gao & Tao Huang

  2. Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, People’s Republic of China

    Jian Zhang

  3. Institute of Biological Engineering of Traditional Chinese Medicine, Tianjin University of Science and Technology, Tianjin, 300457, People’s Republic of China

    Tian-Tian Wen

  4. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, People’s Republic of China

    Lu-Qi Huang

Authors
  1. Juan Wang
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  2. Wen-Yuan Gao
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  3. Jian Zhang
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  4. Tao Huang
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  5. Tian-Tian Wen
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Corresponding author

Correspondence to Wen-Yuan Gao.

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Communicated by E. Lojkowska.

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Wang, J., Gao, WY., Zhang, J. et al. Combination effect of lactoalbumin hydrolysate and methyl jasmonate on ginsenoside and polysaccharide production in Panax quinquefolium L. cells cultures. Acta Physiol Plant 33, 861–866 (2011). https://doi.org/10.1007/s11738-010-0611-x

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  • DOI: https://doi.org/10.1007/s11738-010-0611-x

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