Abstract
The Arabidopsis Ethylene-Insensitive 2 (EIN2) gene was shown to be involved in the regulation of abiotic and biotic stresses, such as ozone stress, high salt, oxidative stress, lead and disease resistances. However, it is unclear whether EIN2 plays a role in mediating the ultraviolet (UV)-B response in Arabidopsis. Here, we show that EIN2 is involved in the regulation of the UV-B response. The EIN2 expression was repressed by UV-B exposure, and ein2-1 mutant plants were more tolerant to UV-B than wild-type plants, as indicated by analysis of survival rates. Significant higher increases in flavonoids and anthocyanins were detected in ein2-1 plants than in wild-type plants treated with or without UV-B treatment, which was associated, at least in part, with constitutively increased transcript levels of two key genes CHALCONE SYNTHASE (CHS) and CINNAMATE 4-HYDROXYLASE (C4H) involved in the biosynthesis pathway of flavonoid and anthocyanins. These results suggest that EIN2 mediates the UV-B response, at least in part, via the modulation of expression of CHS and C4H genes.
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Abbreviations
- C4H :
-
CINNAMATE 4-HYDROXYLASE
- CHS :
-
CHALCONE SYNTHASE
- EIN2 :
-
Ethylene-insensitive 2
- MS :
-
Murashige and Skoog
- MYB4 :
-
Myb transcription factor 4
- RT :
-
Reverse transcription
- SAD2 :
-
SENSITIVE TO ABA AND DROUGHT 2
- Uli3 :
-
UV-light-insensitive 3
- UV :
-
Ultraviolet
- UVR8 :
-
UV resistance locus 8
- WT:
-
Wild-type
References
Alonso JM, Hirayama T, Roman G, Nourizadeh S, Ecker JR (1999) EIN2, a bifunctional transducer of ethylene and stress responses in Arabidopsis. Science 284:2148–2152
Bharti AK, Khurana JP (1997) Mutants of Arabidopsis as tools to understand the regulation of phenylpropanoid pathway and UVB protection mechanisms. Photochem Photobiol 65:765–776
Bieza K, Lois R (2001) An Arabidopsis mutant tolerant to lethal ultraviolet-B levels shows constitutively elevated accumulation of flavonoids and other phenolics. Plant Physiol 126:1105–1115
Cakırlar H, Ciçek N, Fedina I, Georgieva K, Doğru A, Velitchkova M (2008) NaCl induced cross-acclimation to UV-B radiation in four Barley (Hordeum vulgare L.) cultivars. Acta Physiol Plant 30:561–567
Cao SQ, Jiang ST, Zhang RX (2006) Evidence for a role of EIN2 gene in the regulation of the oxidative stress response in Arabidopsis. Acta Physiol Plant 28:417–425
Cao SQ, Chen ZY, Liu GQ, Jiang L, Yuan HB, Ren G, Bian XH, Jian HY, Ma XL (2009) The Arabidopsis Ethylene-Insensitive 2 gene is required for lead resistance. Plant Physiol Biochem 47:308–312
Chappell J, Hahlbrock K (1984) Transcription of plant defense genes in response to UV light or fungal elicitor. Nature 311:76–78
Frohnmeyer H, Staiger D (2003) Ultraviolet-B radiation-mediated responses in plants. Balancing damage and protection. Plant Physiol 133:1420–1428
Guzmán P, Ecker JR (1990) Exploiting the triple response of Arabidopsis to identify ethylene-related mutants. Plant Cell 2:513–523
Hahlbrock K, Scheel D (1989) Physiology and molecular biology of phenylpropanoid metabolism. Annu Rev Plant Physiol Plant Mol Biol 40:347–369
Jansen MAK, Gaba V, Greenberg BM (1998) Higher plants and UV-B radiation: balancing damage, repair and acclimation. Trends Plant Sci 3:131–135
Jin H, Cominelli E, Bailey P, Parr A, Mehrtens F, Jones J, Tonelli C, Weisshaar B, Martin C (2000) Transcriptional repression by AtMYB4 controls production of UV-protecting sunscreens in Arabidopsis. EMBO J 19:6150–6161
Kliebenstein DJ, Lim JE, Landry LG, Last RL (2002) Arabidopsis UVR8 regulates ultraviolet-B signal transduction and tolerance and contains sequence similarity to human regulator of chromatin condensation 1. Plant Physiol 130:234–243
Landry LG, Chapple CCS, Last RL (1995) Arabidopsis mutants lacking phenolic sunscreens exhibit enhanced ultraviolet-B injury and oxidative damage. Plant Physiol 109:1159–1166
Li J, Ou-Lee TM, Raba R, Amundson RG, Last RL (1993) Arabidopsis flavonoid mutants are hypersensitive to UV-B irradiation. Plant Cell 5:171–179
Lois R, Buchanan BB (1994) Severe sensitivity to ultraviolet radiation in an Arabidopsis mutant deficient in flavonoid accumulation. Planta 4:504–509
Ormrod DP, Landry LG, Conklin PL (1995) Shortterm UV-B radiation and ozone exposure effects on aromatic secondary metabolite accumulation and shoot growth of flavonoid dericient Arabidopsis mutants. Physiol Plant 93:602–610
Thomma BP, Eggermont K, Tierens KF, Broekaert WF (1999) Requirement of functional ethylene-insensitive 2 gene for efficient resistance of Arabidopsis to infection by Botrytis cinerea. Plant Physiol 121:1093–1102
Wang YN, Liu C, Li KX, Sun FF, Hu HZ, Li X, Zhao YK, Han CY, Zhang WS, Duan YF, Liu MY, Li X (2007) Arabidopsis EIN2 modulates stress response through abscisic acid response pathway. Plant Mol Bio 64:633–644
Yuan JS, Reed A, Chen F, Stewart CN (2006) Statistical analysis of real-time PCR data. BMC Bioinformatics 7:85
Zhao JF, Zhang WH, Zhao Y, Gong XM, Guo L, Zhu GL, Wang XC, Gong ZZ, Schumaker KS, Guo Y (2007) SAD2, an importin β-Like protein, is required for UV-B response in Arabidopsis by mediating MYB4 nuclear trafficking. Plant Cell 19:3805–3818
Acknowledgments
We thank Jiajia Sun, Kai Zong, Yang Wang, and Jizhi Zhou for their technical assistances. This work was supported by the National Natural Science Foundation of China (grant no. 20777014), the National Key Technology R&D Program of China (grant no. 2008BADC0B00), and the National Transgenic Plant R&D Project of China (2009ZX08009-063B).
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Communicated by J. Sadowski.
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Sun, Z., Wen, C., Chen, Z. et al. A role for Ethylene-Insensitive 2 gene in the regulation of the ultraviolet-B response in Arabidopsis. Acta Physiol Plant 33, 1025–1030 (2011). https://doi.org/10.1007/s11738-010-0593-8
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DOI: https://doi.org/10.1007/s11738-010-0593-8