Advertisement

Acta Physiologiae Plantarum

, Volume 33, Issue 2, pp 473–480 | Cite as

Influence of freezing and non-freezing temperature on somatic embryogenesis and vinblastine production in Catharanthus roseus (L.) G. Don.

  • Junaid Aslam
  • A. MujibEmail author
  • M. P. Sharma
Original Paper

Abstract

Catharanthus roseus (L.) G. Don. (Apocynaceae) is an important dicotyledonous medicinal plant. It produces vinblastine and vincristine, two alkaloids that are being used against a variety of cancers. In the present study, the freezing (−196, 4, 15°C) and non-freezing (25°C) temperature was imposed on embryogenic cultures, and later in vitro embryogeny and vinblastine production in C. roseus was studied. Somatic embryo (SE) production was maximum at 15°C, but the SE maturation was high at 4°C. The SEs, grown at 25°C, showed highest germination and plantlet conversion. Quantitative estimation of vinblastine was carried out using high-performance liquid chromatography in various in vitro raised tissues (embryogenic callus), embryo stages (proliferated, matured and germinated embryos)], and SE-derived plantlets (leaf, shoot, root and whole plant) after various freezing- and non-freezing temperature treatments. Vinblastine synthesis was temperature dependent in C. roseus that has been discussed in this present article.

Keywords

Catharanthus roseus High-performance liquid chromatography Anticancerous alkaloids 

Notes

Acknowledgments

The first author (Dr. Junaid Aslam) gratefully acknowledges the Head, Department of Botany, F/O Science, Hamdard University, New Delhi, INDIA, for providing the necessary facilities during the course of present investigation.

References

  1. Bewley JD, Black M (1994) Seeds: physiology of development and germination. Springer, BerlinGoogle Scholar
  2. Bhat MA, Ahmad S, Junaid A, Mujib A, Mahmooduzzafar (2008) Salinity stress enhanced production of solasodine in Solanum nigrum L. Chem Pharm Bull 56(1):17–21Google Scholar
  3. Carraway DT, Merkle SA (1997) Plantlet regeneration from somatic embryos of American chestnut. Can J For Res 27:1805–1812CrossRefGoogle Scholar
  4. Cheong EJ, Pooler MR (2004) Factors affecting somatic embryogenesis in Prunus incisa cv. February Pink. Plant Cell Rep 22:810–815CrossRefPubMedGoogle Scholar
  5. Chu I, Bodnar JA, White EL, Bowman RN (1996) Quantification of vincristine and vinblastine in Catharanthus roseus plants by capillary zone electrophoresis. J Chromatogr 755:281–288CrossRefGoogle Scholar
  6. Corredoira E, Ballester A, Vieitez AM (2003) Proliferation, maturation and germination of Castanea sativa Mill somatic embryos originated from leaf explants. Anal Bot 92:129–136CrossRefGoogle Scholar
  7. Datta A, Srivastava PS (1997) Variation in vinblastine production by Catharanthus roseus during in vivo and in vitro differentiation. Phytochemistry 46:135–137CrossRefGoogle Scholar
  8. Engelmann F (1997) In vitro conservation methods. In: Callow JA, Ford-Lloyd BV, Newbury HJ (eds) Biotechnology and plant genetic resources. CAB International, Oxford, pp 119–161Google Scholar
  9. Favretto D, Piovan A, Filippini R, Caniato R (2001) Monitoring the production yields of vincristine and vinblastine in Catharanthus roseus from somatic embryogenesis. Semiquantitative determination by flow-injection electrospray ionization mass spectrometry. Rapid Commun Mass Spectrom 15(5):364–369CrossRefPubMedGoogle Scholar
  10. Hagmann HM, Ryynanen LA, Aronen TS, Krajinakova J (1998) Cryopreservation of embryogenic cultures of Scots pine. Plant Cell Tissue Organ Cult 54:45–53CrossRefGoogle Scholar
  11. Hincha DK, Zuther E, Hundertmark M, Heyer AG (2006) The role of compatible solutes in plant freezing tolerance: a case study on Raffinose. In: Chen THH, Uemura M, Fujikawa S (eds) Cold hardiness in plants: molecular genetics, cell biology and physiology. CAB International, Oxford, pp 203–217Google Scholar
  12. Holliday C, Merkle SA (2000) Preservation of American chestnut germplasm by cryostorage of embryogenic cultures. J Am Chestnut Found 14:46–52Google Scholar
  13. Joy IV, Yeung RWEC, Kong L, Thorpe TA (1991) Development of white spruce somatic embryos: I. Storage product deposition. In Vitro Cell Dev Biol Plant 27:32–41CrossRefGoogle Scholar
  14. Junaid A, Bhatt MA, Mujib A, Sharma MP (2006) Somatic embryo proliferation, maturation and germination in Catharanthus roseus. Plant Cell Tissue Organ Cult 84:325–332CrossRefGoogle Scholar
  15. Junaid A, Mujib A, Sharma MP, Samaj J (2007a) Somatic embryogenesis and plant regeneration in Catharanthus roseus. Biol Plant 51(4):641–646CrossRefGoogle Scholar
  16. Junaid A, Mujib A, Sharma MP, Tang W (2007b) Growth regulators affect primary and secondary somatic embryogenesis in Madagaskar periwinkle (Catharanthus roseus (L.) G Don.) at morphological and biochemical levels. Plant Growth Regul 51:271–281CrossRefGoogle Scholar
  17. Junaid A, Mujib A, Fatima S, Sharma MP (2008) Culture condition affect somatic embryogenesis in Catharanthus roseus L (G.) Don. Plant Biotechnol Rep 2:179–190CrossRefGoogle Scholar
  18. Junaid A, Mujib A, Nasim SA, Sharma MP (2009) Screening of vincristine yield in ex vitro and in vitro somatic embryos derived plantlets of Catharanthus roseus L. (G) Don. Sci Hortic 119:325–329CrossRefGoogle Scholar
  19. Karam NS, Al-Majathoub M (2000) Direct shoot regeneration and microtuberization in wild Cyclamen persicum Mill using seedling tissue. Sci Hortic 86:235–246CrossRefGoogle Scholar
  20. Kuboyama T, Yokoshima S, Tokuyama H, Fukuyama T (2004) Stereocontrolled total synthesis of (+) vincristine. PNAS 101:11966–11970CrossRefPubMedGoogle Scholar
  21. Lizbeth A, Castro-Concha L, Maria Escobedo R, de Lourdes Miranda-Ham M (2005) Measurement of cell viability protocol. Plant Cell Cult Protocol 318:71–76Google Scholar
  22. Mathur G, Alkutkar VA, Nadgauda RS (2003) Cryopreservation of embryogenic culture of Pinus roxburghii. Biol Plant 46:205–210CrossRefGoogle Scholar
  23. Meijer AH, Verpoorte R, Hoge JHC (1993) Regulation of enzymes and genes involved in terpenoids indole alkaloid biosynthesis in Catharanthus roseus. J Plant Res 3:145–164Google Scholar
  24. Merkle SA, Parrot WA, Flinn BS (1995) Morphogenetic aspects of somatic embryogenesis. In: Thorpe TA (ed) In vitro embryogenesis in plants. Kluwer, Dordrecht, pp 155–203Google Scholar
  25. Miura Y, Hirata K, Kurano KN, Miyamoto K, Chiida K (1988) Formation of vinblastine in multiple shoot culture of Catharanthus roseus. Planta Med 54:18–20CrossRefPubMedGoogle Scholar
  26. Moreno PRH, Van der Heijden R, Verpoorte R (1995) Cell, tissue cultures of Catharanthus roseus; a literature survey II. Updating from 1988–1993. Plant Cell Tissue Organ Cult 42:1–25CrossRefGoogle Scholar
  27. Moriguchi T, Kozaki I, Yamaki S, Sanada T (1990) Low temperature storage of Pear shoots in vitro. Bull Fruit Tree Res Stn 17:11–18Google Scholar
  28. Mujib A, Samaj J (2006) Somatic embryogenesis. Springer, BerlinCrossRefGoogle Scholar
  29. Mukherjee AK, Basu S, Sarkar N, Ghosh AC (2001) Advances in cancer therapy with plant based natural products. Curr Med Chem 8:1467–1486PubMedGoogle Scholar
  30. Pan ZG, Liu CZ, Murch SJ, El-Demerdash M, Saxena PK (2003) Plant regeneration from mesophyll protoplasts of the Egyptian medicinal plants Artemisia judaica L. and Echinops spinosissimus Turra. Plant Sci 165:681–687CrossRefGoogle Scholar
  31. Pande D, Purohit M, Srivastava PS (2002) Variation in xanthotoxin content in Ammi majus L. cultures during in vitro flowering and fruiting. Plant Sci 162:583–587CrossRefGoogle Scholar
  32. Pasquali G, Goddijin OJM, De Wall A, Verpoorte R, Schilperoort RA, Hoge JHC (1992) Coordinated regulation of two indole alkaloid biosynthetic genes from Catharanthus roseus by auxin and elicitors. Plant Mol Biol 18:1121–1131CrossRefPubMedGoogle Scholar
  33. Pullman GS, Gupta PK, Timmis R, Carpenter C, Kreitinger M, Welty E (2005) Improved Norway spruce somatic embryo development through the use of abscisic acid combined with activated carbon. Plant Cell Rep 24:271–279CrossRefPubMedGoogle Scholar
  34. Ramage CM, Williams RR (2004) Cytokinin induced abnormal shoot regeneration is associated with elevated knottedl—type homeobox gene expression in tobacco. Plant Cell Rep 22:919–924CrossRefPubMedGoogle Scholar
  35. San-José MC, Ballester A, Vieitez AM (2001) Effect of thidiazuron on multiple shoot induction and plant regeneration from cotyledonary nodes of chestnut. J Hortic Sci Biotechnol 76:588–595Google Scholar
  36. Stasolla C, Loukanina N, Ashihara H, Yeung EC, Thorpe TA (2002) Pyrimidine nucleotide biosynthesis and nucleic acid metabolism in embryos and megagametophytes of white spruce (Picea glauca) during germination. Physiol Plant 115:155–165CrossRefPubMedGoogle Scholar
  37. Thorpe TA (1995) In vitro embryogenesis in plants. Kluwer, DordrechtGoogle Scholar
  38. Van der Heijden R, Verpoorte R, Ten Hoopen HJG (1989) Cell and tissue cultures of Catharanthus roseus (L) G. Don: a literature survey. Plant Cell Tissue Organ Cult 18:231–280CrossRefGoogle Scholar
  39. Van der Heijden R, Jacobs DT, Snoeijer W, Hallard D, Verpoorte R (2004) The Catharanthus alkaloids: pharmacognosy and biochemistry. Curr Med Chem 11:607–628CrossRefGoogle Scholar
  40. Verpoorte R, Van der Heijden R, Schripsema J, Hoge JHC, Ten Hoopen HJG (1993) Plant biotechnology for the production of alkaloids: present status and prospects. J Nat Prod 56:186–207CrossRefGoogle Scholar
  41. Winkelmann T, Mubmann V, Serek M (2004) Cryopreservation of embryogenic suspension cultures of Cyclamen persicum. Mill. Plant Cell Rep 23:1–8CrossRefPubMedGoogle Scholar
  42. Xiao L, Koster KL (2001) Desiccation tolerance of protoplasts isolated from pea embryos. J Exp Bot 52:2105–2114PubMedGoogle Scholar
  43. Xin Z, Browse J (2000) Cold comfort farm: the acclimation of plants to freezing temperatures. Plant Cell Environ 23:893–902CrossRefGoogle Scholar
  44. Xing Z, Powell WA, Maynard CA (1999) Development and germination of American chestnut somatic embryos. Plant Cell Tissus Organ Cult 57:47–55CrossRefGoogle Scholar

Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2010

Authors and Affiliations

  1. 1.Cellular Differentiation and Molecular Genetic Section, Department of BotanyHamdard UniversityNew DelhiIndia

Personalised recommendations