Abstract
An efficient micropropagation protocol was established for Cryptocoryne wendtii and Cryptocoryne becketti using shoot tips explants. Multiple shoots were induced from shoot tip explants of both species cultured on agar-gelled as well as liquid MS medium supplemented with 0.5 mg/L BA and 0.2 mg/L IBA (proliferation medium). The multiple shoots of both the species formed on agar-gelled as well as liquid medium were vigorously growing with well-developed roots and leaves after 4 weeks of culture. Highest number of multiple shoots was obtained from shoot tip explants of both the species cultured in liquid proliferation medium after 4 weeks of culture. The shoot tip explants of C. wendtii and C. becketti, that were cultured in liquid proliferation medium (2 weeks) followed by culturing on agar-gelled proliferation medium (2 weeks) also produced the multiple shoots. Shoot tips cultured on agar-gelled medium produced the least number of multiple shoots after 4 weeks of culture. Histological study did not show any abnormalities in the leaves of in vitro plantlets of both the species, cultured in agar-gelled and liquid proliferation medium. The leaves of the in vitro plantlets formed normal mesophyll cells and vascular bundles. More than 95% of the acclimatized plantlets grew vigorously without any morphological abnormalities.
Abbreviations
- MS:
-
Murashige and Skoog medium
- BA:
-
6-Benzyladenine
- IBA:
-
3-Indole butyric acid
References
Bhatt ID, Dhar U (2000) Micropropagation of Indian wild strawberry. Plant Cell Tissue Organ Cult 6:83–88
Burkill IH (1966) A dictionary of economic products of the Malay Peninsula. vol. 1 (A-H). The Ministry of Agriculture and Co-operatives, Kuala Lumpur p 704
Chan LK, Thong WH (2004) In vitro propagation of Zingiberaceae species with medicinal properties. J Plant Biotechnol 6(3):181–188
Cutler DF, Botha T, Stevenson DW (2007). Plant anatomy: an applied approach. Blackwell USA, p 93
Dötsch A (1984) Cryptocoryne wendtii de Wit (Pflanzenportrait). Aqua Planta 84(3):17–18
Huang L, Chang Y, Chang Y (1994) Rapid in vitro multiplication of the aquatic angiosperm, Anubias barteri var undulata. Aquat Bot 47:77–83
Jenks MA, Kane ME, McConnell DB (2000) Shoot organogenesis from petiole explants in the aquatic plant Nymphoides indica. Plant Cell Tissue Organ Cult 63:1–8
Johansen DA (1940) Plant microtechnique. McGraw-Hill, New York
Kadota M, Nimii Y (2004) Improvement of micropropagation of Japanese yam using liquid and gelled medium culture. Sci Hortic 102(4):461–466
Kane ME, Gilman EF, Jenks MA, Sheehan TJ (1990) Micropropagation of the aquatic plant Cryptocoryne lucens. Hort Sci 25:687–689
Kane ME, Davis GL, McConnell DB, Gargiulo JA (1999) In vitro propagation of Cryptocoryne wendtii. Aquat Bot 63:197–202
Kukulczanka K, Klimaszewska K, Slawinski S (1980) In vitro culture of Aponogeton. Trop Fish Hobbyist 29:36–39
Lee WL, Chan LK (2004) Plant regeneration from stem nodal segments of Orthosiphon stamineus Benth., A medicinal plant with diuretic activity. In Vitro Cell Dev Biol Plant 40:115–118
Mansor M, Masnadi M (1994) Cryptocoryne elliptica, an endangered amphibious plant in Pondok-Tanjung Forest Reserve, peninsular Malaysia. Aquat Bot 47:91
Micheli M, De Gasperis A, Prosperi F, Standardi A (2006) Micropropagation of three species of aquatic plants. Agricoltura Mediterranea (Italy) 131(1):46–51
Mo X, Jiang Q (2003) Study on rapid proliferation and color appearing of tropical aquatic grass Cryptocoryne wendtii. Acta Agric Zhejiangensis 15(2):91–94
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Plant Physiol 15:473–497
Ozturk M, Khawar KM, Atar HH, Sancak C, Ozcan S (2004) In vitro micropropagation of aquarium plant Ludwigia repens. Asia Pac J Mol Biol Biotechnol 12(1&2):21–25
Petersen M (1996) Tissue culture propogation of aquarium plant—New prospects? OFI J 14:8–9
Piatczak E, Wielanek M, Wysokinska H (2005) Liquid culture system for shoot multiplication and secoiridoid production in micropropagated plants of Centaurium erythraea Rafn. Plant Sci 168:431–437
Rataj R, Horeman TJ (1977) Aquarium Plants. T.F.H. Publications, Neptune City
Rosen DJ (2000) Cryptocoryne beckettii (Araceae), a new aquatic plant in Texas. Sidahora 19(2):399–401
Sudhersan C, AboEL-Nil M, Hussain J (2003) Tissue culture technology for the conservation and propagation of certain native plants. J Arid Environ 54:133–147
Sulaiman ES (2004) Development of sterilization procedures and in vitro studies of Nymphaea lotus. MSc Thesis. Universiti Putra Malaysia
Windelov H (1987) Aquarium plants. T. F. H. Publications, Neptune City
Zacchini M, De Agazio M (2004) Micropropagation of a local olive cultivar for germplasm preservation. Biol Plant 48(4):589–592
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Communicated by E. Lojkowska.
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Stanly, C., Bhatt, A. & Keng, C.L. An efficient in vitro plantlet regeneration of Cryptocoryne wendtii and Cryptocoryne becketti through shoot tip culture. Acta Physiol Plant 33, 619–624 (2011). https://doi.org/10.1007/s11738-010-0541-7
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DOI: https://doi.org/10.1007/s11738-010-0541-7