Acta Physiologiae Plantarum

, Volume 32, Issue 6, pp 1047–1053 | Cite as

The impact of zearalenone and thidiazuron on indirect plant regeneration of oilseed rape and wheat

  • Jolanta Biesaga-KościelniakEmail author
  • Janusz Kościelniak
  • Anna Janeczko
Original Paper


The aim of the study was to test the synergistic activity of zearalenone and thidiazuron with commonly used regulators (6-benzylaminopurine, 2,4-dichlorophenoxyacetic acid) in callus tissue growth and the regeneration processes of winter oilseed rape and winter wheat. Oilseed rape callus was obtained from the upper fragments of seedling hypocotyls. Wheat callus tissue was obtained from immature embryos. Zearalenone, in combination with 6-benzylaminopurine, stimulated the regeneration of oilseed rape and, in combination with 2,4-dichlorophenoxyacetic acid, the regeneration of wheat. An additional application of thidiazuron to these media increased the percentage of shoot regeneration from callus in both species tested. Zearalenone is proposed as a new regulator in in vitro plant cultures for use in protocols of plant regeneration.


Brassica napus Callus Growth regulators Hypocotyls Immature embryos Triticum aestivum 





2,4-Dichlorophenoxyacetic acid







The authors would like to express their gratitude to Dr. Iwona Żur (Institute of Plant Physiology, PAS, Krakow) for taking a photo of the in vitro culture of oilseed rape.


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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2010

Authors and Affiliations

  • Jolanta Biesaga-Kościelniak
    • 1
    Email author
  • Janusz Kościelniak
    • 2
  • Anna Janeczko
    • 1
  1. 1.Institute of Plant PhysiologyPolish Academy of SciencesKrakówPoland
  2. 2.Faculty of AgricultureUniversity of AgricultureKrakówPoland

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