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The impact of zearalenone and thidiazuron on indirect plant regeneration of oilseed rape and wheat

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Abstract

The aim of the study was to test the synergistic activity of zearalenone and thidiazuron with commonly used regulators (6-benzylaminopurine, 2,4-dichlorophenoxyacetic acid) in callus tissue growth and the regeneration processes of winter oilseed rape and winter wheat. Oilseed rape callus was obtained from the upper fragments of seedling hypocotyls. Wheat callus tissue was obtained from immature embryos. Zearalenone, in combination with 6-benzylaminopurine, stimulated the regeneration of oilseed rape and, in combination with 2,4-dichlorophenoxyacetic acid, the regeneration of wheat. An additional application of thidiazuron to these media increased the percentage of shoot regeneration from callus in both species tested. Zearalenone is proposed as a new regulator in in vitro plant cultures for use in protocols of plant regeneration.

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Abbreviations

BAP:

6-Benzylaminopurine

2,4-D:

2,4-Dichlorophenoxyacetic acid

ZEN:

Zearalenone

TDZ:

Thidiazuron

References

  • Bahieldin A, Dyer WE, Qu R (2000) Concentration effects of dicamba on shoot regeneration in wheat. Plant Breed 119:437–439

    Article  CAS  Google Scholar 

  • Biesaga-Kościelniak J (1998) Investigation of the possibility of stimulating generative development of plants by exogenous zearalenone. Acta Physiol Plant 20:4–5

    Google Scholar 

  • Biesaga-Kościelniak J (2001) Zearalenone as a new hypothetical regulator of plant growth and development. Monograph of Institute of Plant Physiology, Polish Academy of Sciences, Krakow Poland (in Polish, with English summary, description of tables and figures)

  • Biesaga-Kościelniak J, Filek M (2010) Occurrence and physiology of zearalenone as a new plant hormone. In: Lichtfouse E (ed) Sustainable agriculture reviews 3: sociology, organic farming, climate changing and soil science. Springer, Dordrecht, pp 419–437

  • Biesaga-Kościelniak J, Marcińska I, Wędzony M, Kościelniak J (2003) Effect of zearalenone treatment in the production of wheat haploids via maize pollination system. Plant Cell Rep 21:1035–1039

    Article  PubMed  Google Scholar 

  • Bohorova NE, Pfeiffer WH, Mergoum M, Crossa J, Pacheco M, Estañol P (2001) Regeneration potential of CIMMYT durum wheat and triticale varieties from immature embryos. Plant Breed 120:291–295

    Article  CAS  Google Scholar 

  • Borkowska B, Litwińczuk W (1993) Activity of thidiazuron in vitro shoot cultures of Prunus sp and Morus alba. Biol Plant 35:63–67

    Article  CAS  Google Scholar 

  • Capelle SC, Mok DWS, Kirchner SC, Mok MC (1983) Effect of thidiazuron on cytokinin autonomy and the metabolism on N6-(isopentenylo[8–14C] adenosine in callus tissues of Phaseolus lunatus L. Plant Physiol 80:449–493

    Google Scholar 

  • Chuong PV, Pauls KP, Beversdorf WD (1985) A simple culture method for Brassica hypocotyl protoplasts. Plant Cell Rep 4:4–6

    Article  Google Scholar 

  • De Jong AJ, Schmidt DEDL, De Veries SC (1993) Early events in higher-plant embryogenesis. Plant Mol Biol 22:367–377

    Article  PubMed  Google Scholar 

  • Ganeshan S, Chodaparambil SV, Båga M, Fowler DB, Hucl P, Rossnagel BG, Chibbar RN (2006) In vitro regeneration of cereals based on multiple shoot induction from mature embryos in response to thidiazuron. Plant Cell Tiss Org Cult 85:63–73

    Article  CAS  Google Scholar 

  • Gill R, Saxena PK (1993) Somatic embryogenesis in Nicotiana tabacum L: induction by thidiazuron of direct embryo differentiation from cultured leaf discs. Plant Cell Rep 12:154–159

    Article  Google Scholar 

  • Gubbuk H, Pekmezci M (2006) In vitro propagation of banana (Musa spp.) using thidiazuron and activated charcoal. Acta Agric Scand B Soil Plant Sci 56:65–69

    CAS  Google Scholar 

  • Hutchinson MJ, Murch SJ, Saxena PK (1996) Morphoregulatory role of thidiazuron: evidence of the involvement of endogenous auxin in thidiazuron-induced somatic embryogenesis of geranium (Pelargonium × hortorum Bailey). J Plant Physiol 149:573–579

    CAS  Google Scholar 

  • Jiang B, Yang YG, Guo YM, Guo ZC, Chen YZ (2005) Thidiazuron-induced in vitro shoot organogenesis of the medicinal plant Arnebia euchroma (Royle) Johnst. In Vitro Cell Dev Biol Plant 41:677–681

    Article  CAS  Google Scholar 

  • Jones MPA, Yi Z, Murch SJ, Saxena PK (2007) Thidiazuron-induced regeneration of Echinacea purpurea L.: micropropagation in solid and liquid culture systems. Plant Cell Rep 26:13–19

    Article  CAS  PubMed  Google Scholar 

  • Khehra GS, Mathias RJ (1992) The interaction of genotype, explant and media on the regeneration of shoots from complex explants of Brassica napus L. J Exp Bot 43:1413–1418

    Article  Google Scholar 

  • Khurana J, Chugh A, Khurana P (2002) Regeneration from mature and immature embryos and transient gene expression via Agrobacterium-mediated transformation in emmer wheat (Triticum dicoccum Schuble). Ind J Exp Biol 40:1295–1303

    CAS  Google Scholar 

  • Klang DT, Kennedy BJ, Pathre SV, Mirocha CJ (1978) Binding characteristics of zearalenone analogies to estrogen receptors. Cancer Res 38:3611–3616

    Google Scholar 

  • Lazzeri PA, Dunwell JM (1984) In vitro shoot regeneration from seedling root segments of Brassica oleracea and Brassica napus cultivars. Ann Bot 54:341–350

    CAS  Google Scholar 

  • Maheshwari N, Rajyalakshmi K, Baweja K, Dhir SK, Chowdhry CN, Maheshwari SC (1995) In vitro culture of wheat and genetic transformation—retrospect and prospect. Plant Sci 14:49–178

    Google Scholar 

  • Marcińska I (2003) Wheat cell suspension culture. Monograph of Institute of Plant Physiology, Polish Academy of Sciences, Krakow Poland (in Polish, with English summary, description of tables and figures)

  • Marcińska I, Szechyńska M, Biesaga-Kościelniak J, Filek M (2001) Applicability of Polish winter wheat (Triticum aestivum L.) cultivars to long-term in vitro culture. Cereal Res Commun 29:127–134

    Google Scholar 

  • Meng FJ, Han Y, Que Y, Wang H (1992) Zearalenone, a key substance controlling plant development. In: Karssen CM, Loon LCV, Vreugdenhil D (eds) Progress in plant growth regulation. Kluwer Academic Publishers, Dordrecht, pp 291–297

    Google Scholar 

  • Michalczuk L, Cook TJ, Cohen JD (1992) Auxin levels at different stages of carrot somatic embryogenesis. Phytochemistry 31:1097–1103

    Article  CAS  Google Scholar 

  • Muller R, Baier M, Kaiser WM (1991) Differential stimulation of PEP-carboxylation in guard cells and mesophyll cells by ammonium or fusicoccin. J Exp Bot 42:215–220

    Article  Google Scholar 

  • Mundhara R, Rashid A (2002) Stimulation of shoot-bud regeneration on hypocotyl of Linum seedlings, on a transient withdrawal of calcium: effect of calcium, cytokinin and thidiazuron. Plant Sci 162:211–214

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Newberne PM (1987) Interaction of nutrients and other factors with mycotoxins. In: Krough P (ed) Mycotoxin in food. Academic Press, London, p 177

    Google Scholar 

  • Podwyszyńska M, Marasek A (2003) Effect of thidiazuron and pactlobutrazol on regeneration potential of tulip flower stalk explants in vitro and subsequent shoot multiplication. Acta Soc Bot Pol 72:181–190

    Google Scholar 

  • Przetakiewicz A, Orczyk W, Nadolska-Orczyk A (2003) The effect of auxin on plant regeneration of wheat, barley and triticale. Plant Cell Tiss Org Cult 73:245–256

    Article  CAS  Google Scholar 

  • Shan X, Li D, Qu R (2000) Thidiazuron promotes in vitro regeneration of wheat and barley. In Vitro Cell Dev Biol Plant 36:207–210

    Article  CAS  Google Scholar 

  • Sharma VK, Hänsch R, Mendel RR, Schulze J (2005) Influence of picloram and thidiazuron on high frequency plant regeneration in elite cultivars of wheat with long-term retention of morphogenecity using meristematic shoot segments. Plant Breed 124:242–246

    Article  CAS  Google Scholar 

  • Szechyńska-Hebda M, Skrzypek E, Dąbrowska G, Biesaga-Kościelniak J, Filek M, Wędzony M (2007) The role of oxidative stress induced by growth regulators in the regeneration process of wheat. Acta Physiol Plant 29:327–337

    Article  Google Scholar 

  • Thomas TD, Puthur JT (2004) Thidiazuron induced high frequency shoot organogenesis in callus from Kigelia pinnata L. Bot Bull Acad Sin 45:307–313

    CAS  Google Scholar 

  • Visser C, Qureshi JA, Gill R, Saxena PK (1992) Morphoregulatory role of thidiazuron: substitution of auxin and cytokinin requirement for the induction of somatic embryogenesis in geranium hypocotyl cultures. Plant Physiol 99:1704–1707

    Article  CAS  PubMed  Google Scholar 

  • Wang JX, Zhao FY, Xu P (2006) Use of aroA-M1 as a selectable marker for Brassica napus transformation. Crop Sci 46:706–711

    Article  CAS  Google Scholar 

  • Zhang GQ, Zhou WJ, Gu HH, Song WJ, Momoh EJJ (2003) Plant regeneration from the hybridization of Brassica juncea and B. napus through embryo culture. J Agron Crop Sci 189:347–350

    Article  Google Scholar 

  • Żur I, Kościelniak J, Dubert F (2002) Metabolic activity as a possible indicator of morphogenic potential in rape callus cultures—the effect of exogenous spermidine. Zesz Probl Post Nauk Roln 481:357–367 (in Polish, with English abstract, description of tables and figures)

    Google Scholar 

Download references

Acknowledgments

The authors would like to express their gratitude to Dr. Iwona Żur (Institute of Plant Physiology, PAS, Krakow) for taking a photo of the in vitro culture of oilseed rape.

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Authors and Affiliations

  1. Institute of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239, Kraków, Poland

    Jolanta Biesaga-Kościelniak & Anna Janeczko

  2. Faculty of Agriculture, University of Agriculture, Podłużna 3, 30-239, Kraków, Poland

    Janusz Kościelniak

Authors
  1. Jolanta Biesaga-Kościelniak
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  2. Janusz Kościelniak
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  3. Anna Janeczko
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Corresponding author

Correspondence to Jolanta Biesaga-Kościelniak.

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Communicated by B. Borkowska.

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Biesaga-Kościelniak, J., Kościelniak, J. & Janeczko, A. The impact of zearalenone and thidiazuron on indirect plant regeneration of oilseed rape and wheat. Acta Physiol Plant 32, 1047–1053 (2010). https://doi.org/10.1007/s11738-010-0495-9

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  • DOI: https://doi.org/10.1007/s11738-010-0495-9

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