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Modulation of symbiotic efficiency and nodular antioxidant enzyme activities in two Phaseolus vulgaris genotypes under salinity

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Abstract

To analyse nodular expression of antioxidant enzymes depending on plant genotype and salinity, two Phaseolus vulgaris genotypes, tolerant BAT477 and sensitive COCOT, were inoculated with the reference strain Rhizobium tropici CIAT899 and grown under 25 and 50 mM NaCl. Plant growth, nodulation and nitrogen fixing activity measured by the acetylene reducing activity (ARA) as an indicator of nitrogenase (E.C. 1.7.9.92) activity were more affected by salt concentrations in COCOT than in BAT477, particularly with 50 mM NaCl. Electrophoresis analysis of antioxidant enzymes in nodules, roots and free-living rhizobia showed that only catalase (CAT E.C. 1.11.1.6) isoenzymes varied with genotype. The sensitive genotype showed lower antioxidant enzyme activities than tolerant genotype and it was more affected by salinity. In the tolerant genotype catalase and ascorbate peroxidase (APX, E.C. 1.11.1.11) were inhibited by salt stress, whereas superoxide dismutase (SOD, E.C. 1.15.1.1) and peroxidase (POX, E.C. 1.11.1.7) were activated by salinity. Statistical analysis allowed suggesting that tolerance to salinity is associated with a differential regulation of distinct superoxide dismutase and peroxidase activities.

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Abbreviations

APX:

Ascorbate peroxidase

ARA:

Acetylene reducing activity

ASC-GSH cycle:

Ascorbate–glutathione cycle

CAT:

Catalase

EDTA:

Ethylenediaminetetraacetic acid

MTT:

3-(4,5-Dimethlthiazol-2-4)-5-5diphenyl tetrazolium bromide

NBT:

Nitroblue tetrazolium

NDW:

Nodule dry weight

PMSF:

Phenylmethylsulfonyl fluoride

POX:

Guaiacol peroxidase

PVP:

Polyvinyl-pyrrolidone

ROS:

Reactive oxygen species

RDW:

Root dry weight

SDW:

Shoot dry weight

SOD:

Superoxide dismutase

TEMED:

N,N,N′,N′-tetramethyl ethylene diamine

Tris:

Tris (hydroxymethyl) aminomethane

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Acknowledgments

This work was supported by grants from the Tunisian Ministry of Scientific Research, Technology and Competency Development. We are most grateful to Dr. Inoubli T for English revision. We thank Zitoun A, for excellent technical support.

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Authors and Affiliations

  1. Centre of Biotechnology Borj Cedria (CBBC), BP 901, 2050, Hammam Lif, Tunisia

    Salwa Jebara & Moez Jebara

  2. UMR1222 Rhizosphère & Symbiose INRA-AGRO.M, Place Viala, 34060, Montpellier Cedex 01, France

    Jean Jacque Drevon

Authors
  1. Salwa Jebara
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  2. Jean Jacque Drevon
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  3. Moez Jebara
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Correspondence to Moez Jebara.

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Communicated by G. Klobus.

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Jebara, S., Drevon, J.J. & Jebara, M. Modulation of symbiotic efficiency and nodular antioxidant enzyme activities in two Phaseolus vulgaris genotypes under salinity. Acta Physiol Plant 32, 925–932 (2010). https://doi.org/10.1007/s11738-010-0480-3

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  • DOI: https://doi.org/10.1007/s11738-010-0480-3

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