Physiological characterization of ‘stay green’ wheat cultivars during the grain filling stage under field growing conditions

Abstract

Rye (Secale cereale L.) chromosome arm 1RS could delay leaf senescence, and change in H2O2 content is a useful index for weighing the ability to delay the senescence. Two wheat cultivars, Chuannong12 (CN12) and Chuannong 18 (CN18), harboring the wheat–rye 1BL/1RS translocated chromosome were investigated for H2O2 change and physiological index after flowering under field conditions, and MY11, the agronomical parent of both CN12 and CN18, was used as the control. A combined change in the peak value of CdSe/ZnS quantum dot (QD) fluorescence and morphological observation indicated that the H2O2 contents in CN12 and CN18 were generally lower than that in MY11. They both had higher values for net photosynthetic rate (P n), stomatal conductance (G s), \( F_{\text{v}} /F_{\text{m}}^{\prime } \) \( F_{\text{v}}^{\prime } /F_{\text{m}}^{\prime } \), and photochemical quenching of PSII (qP) than MY11 only in the late measurement stage. Some small differences were also observed, such as CN12 and CN18 wheat cultivars having higher and longer photosynthetic competence than MY11 during the grain filling stage, which perhaps resulted from a mechanism for removing oxidative species, especially H2O2.

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Abbreviations

H2O2 :

Hydrogen peroxide

PSII:

Photosystem II

Fv/Fm:

Maximum photochemical efficiency of PSII

\( F_{\text{v}}^{\prime } /F_{\text{m}}^{\prime } \) :

The efficiency of excitation capture by open PSII reaction centers

ROS:

Reactive oxygen species

P n :

The rate of photosynthesis

C i :

Intercellular CO2 concentration

G s :

Stomatal conductance

qP:

Photochemical quenching coefficient

qN:

Non-photochemical quenching coefficient

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Acknowledgments

We express our gratitude for the financial support from the National Natural Science Foundation of China (No. 30971787 and 30730065) and the Fok Ying Tung Educations Foundation (No. 111030).

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Correspondence to Peigao Luo.

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Communicated by A. Aniol.

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Chen, J., Liang, Y., Hu, X. et al. Physiological characterization of ‘stay green’ wheat cultivars during the grain filling stage under field growing conditions. Acta Physiol Plant 32, 875–882 (2010). https://doi.org/10.1007/s11738-010-0475-0

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Keywords

  • Photosynthetic activity
  • Chlorophyll fluorescence
  • H2O2
  • Leaf senescence
  • Stay-green wheat