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Spatial and temporal patterns of net nitrate uptake regulation and kinetics along the tap root of Citrus aurantium

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Abstract

Spatial–temporal variation of the regulation and the kinetics of net nitrate (NO3 −) uptake rate (NNUR) along the tap root of Citrus aurantium L. were analysed. Suberin incrustation in the peripheral cell layers and plasma membrane (PM) H+-ATPase localisation, anatomical and physiological factors involved in NO3 − uptake were also investigated. The results clearly indicated a spatially uniform distribution of the regulation process, accompanied by a temporal heterogeneous pattern of the kinetics of NO3 − uptake along citrus tap root. In particular, kinetic analysis had a biphasic pattern, saturating (high affinity transport system) and linear (low affinity transport system), in response to increasing external NO3 − concentrations in each root region, where 200 μM NO3 − represented the threshold separating these two systems. Kinetic parameters, K m and V max, clearly indicated that apical segments reached the maximum value of induction before basal segments. Hence, the apical root zones, early exhibiting the maximum of potential capacity to absorb the NO3 −, could be considered more efficient than basal root segments for acquiring NO3 − from external solution. Suberin incrustations in the hypodermal cell layer, characterised by uniform fluorescence intensity among the root segments, could be responsible for the unchanged NNUR, while the PM H+-ATPase could explain the temporal pattern of NNUR.

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Acknowledgments

We thank Prof. J.M. Villalba Montoro (Universidad de Còrdoba, Spain) for technical support and suggestions on the manuscript and Dr. Antonino Nicolò for technical support.

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Authors and Affiliations

  1. Dipartimento di Biotecnologie per il Monitoraggio Agroalimentare ed Ambientale (BIOMAA), Università degli Studi ‘Mediterranea’ di Reggio Calabria, Salita Melissari, 89124, Reggio Calabria, Italy

    Agostino Sorgonà, Lugi Di Dio & Maria Rosa Abenavoli

  2. Dipartimento di Biotecnologie Agrarie, Università di Padova, Viale dell’Università 16, 35020, Legnaro (PD), Italy

    Giovanni Cacco

  3. Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, ROC

    Wolfgang Schmidt & Paula Jay Perry

Authors
  1. Agostino Sorgonà
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  2. Giovanni Cacco
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  3. Lugi Di Dio
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  4. Wolfgang Schmidt
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  5. Paula Jay Perry
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  6. Maria Rosa Abenavoli
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Corresponding author

Correspondence to Agostino Sorgonà.

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Communicated by J. Zwiazek.

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Sorgonà, A., Cacco, G., Di Dio, L. et al. Spatial and temporal patterns of net nitrate uptake regulation and kinetics along the tap root of Citrus aurantium . Acta Physiol Plant 32, 683–693 (2010). https://doi.org/10.1007/s11738-009-0447-4

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  • DOI: https://doi.org/10.1007/s11738-009-0447-4

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