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Acta Physiologiae Plantarum

, Volume 32, Issue 3, pp 541–549 | Cite as

The investigation on accumulation levels of proline and stress parameters of the maize (Zea mays L.) plants under salt and water stress

Original Paper

Abstract

The present study was carried out to determine interactive and comparative effects of salinity and water stress on growth, proline accumulation, chlorophyll, carotenoid and macro nutrient content and antioxidative enzymes such as superoxide dismutase (SOD), guaiacol peroxidase (POX), and polyphenol oxidase (PPO) in hydroponically grown maize (Zea mays L.cv DKC647) plants. Plants were treated two salt (NaCl) concentrations and polyethylene glycol 6000 (PEG 6000) to create water stress. The results obtained from this experiment show that high salinity reduced growth through decreasing shoot and root dry and fresh weight, chlorophyll, and carotenoid content, but PEG treatment had no significant effect on this parameters. Under NaCl and PEG 6000 treatment, uptake and translocation of mineral nutrients changed drastically. The high presence of Na+ in nutrient solution affected considerably the plant nutritional requirement, especially influencing the uptake of Ca2+ and K+, which were restricted for competition. Proline accumulation, and SOD, POX and PPO activities were increased with the increasing intensity of NaCl stress, but PEG 6000 treatment in addition to NaCl had more significant effect on this enzyme activities. These results suggest that maize plants may be increased proline content to maintain osmotic adjustment and increased the activity of antioxidant enzymes to have a better protection against active oxygen species (AOS) under salt and water stress.

Keywords

Salinity and water stress Polyethylene glycol 6000 Proline Antioxidative enzymes (SOD, POX, PPO) Maize 

Abbreviations

AOS

Active oxygen species

BSA

Bovine serum albumin

EC

Electrical conductivity

H2O2

Hydrogen peroxide

MPa

Megapascal

NBT

Nitroblue tetrazolium

OH

Hydroxyl radical

O2•−

Superoxide radical

PEG 6000

Polyethylene glycol 6000

POD

Guaiacol peroxidase

PPO

Polyphenol oxidase

PVP

Polyvinylpyrrolidone

SOD

Superoxide dismutase

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Copyright information

© Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków 2010

Authors and Affiliations

  1. 1.Department of Biology, Faculty of Science and ArtMuğla UniversityMuğlaTurkey

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