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The effect of zinc on the growth and physiological and biochemical parameters in seedlings of Eruca sativa (L.) (Rocket)

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Abstract

Eruca sativa seedlings were treated with different Zn concentrations (0, 250, 500, 1,000, 2,000 μg g−1 dried growth medium) under controlled conditions. The seedlings were harvested 20 days after Zn treatment. Physiological parameters, such as root and shoot length, fresh and dry weight, were measured and Zn content of roots and shoots was determined. Furthermore, various biochemical parameters were studied on E. sativa leaves: enzymatic antioxidants, such as superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (POD), ascorbate peroxidase (APX), and non-enzymatic antioxidants, such as ascorbate, non-protein thiols. Malondialdehyde, which is an index of lipid peroxidation, was assayed. Zn treatment did not have any significant effect on fresh and dry weights, whereas 500 μg g−1 Zn increased root growth significantly (p < 0.05). Zn accumulated in roots 2–8 times more than it did in leaves. Lipid peroxidation increased in proportion with the increase in Zn. Although a decrease in SOD and CAT activities at increased Zn was found, a significant increase in APX and POD was observed at 500 and 1,000 μg g−1 Zn, respectively. In addition, an increase in the amounts of non-protein thiols and total AsA (Ascorbate) was observed with the increase in Zn.

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Acknowledgments

This work was supported by Ondokuz Mayis University Scientific Research Foundation (Project Number: F- 387). The authors acknowledge the financial support of Ondokuz Mayis University.

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Authors and Affiliations

  1. Department of Biology, Faculty of Sciences and Arts, University of Ondokuz Mayis, Kurupelit, 55139, Samsun, Turkey

    Yasemin Ozdener

  2. Department of Biology, Faculty of Science and Arts, University of Amasya, 05100, Amasya, Turkey

    Birsen K. Aydin

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  1. Yasemin Ozdener
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Correspondence to Yasemin Ozdener.

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Communicated by G. Bartosz.

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Ozdener, Y., Aydin, B.K. The effect of zinc on the growth and physiological and biochemical parameters in seedlings of Eruca sativa (L.) (Rocket). Acta Physiol Plant 32, 469–476 (2010). https://doi.org/10.1007/s11738-009-0423-z

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  • DOI: https://doi.org/10.1007/s11738-009-0423-z

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