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Elevated CO2 ameliorated oxidative stress induced by elevated O3 in Quercus mongolica

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Abstract

Using open top chambers, the effects of elevated O3 (80 nmol mol−1) and elevated CO2 (700 μmol mol−1), alone and in combination, were studied on young trees of Quercus mongolica. The results showed that elevated O3 increased malondialdehyde content and decreased photosynthetic rate after 45 days of exposure, and prolonged exposure (105 days) induced significant increase in electrolyte leakage and reduction of chlorophyll content. All these changes were alleviated by elevated CO2, indicating that oxidative stress on cell membrane and photosynthesis was ameliorated. After 45 days of exposure, elevated O3 stimulated activities of superoxide dismutase (SOD, EC 1.15.1.1) and ascorbate peroxidase (APX, EC 1.11.1.11), but the stimulation was dampened under elevated CO2 exposure. Furthermore, ascorbate (AsA) and total phenolics contents were not higher in the combined gas treatment than those in elevated O3 treatment. It indicates that the protective effect of elevated CO2 against O3 stress was achieved hardly by enhancing ROS scavenging ability after 45 days of exposure. After 105 days of exposure, elevated O3 significantly decreased activities of SOD, catalase (CAT, EC 1.11.1.6) and APX and AsA content. Elevated CO2 suppressed the O3-induced decrease, which could ameliorate the oxidative stress in some extent. In addition, elevated CO2 increased total phenolics content in the leaves both under ambient O3 and elevated O3 exposure, which might contribute to the protection against O3-induced oxidative stress as well.

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Abbreviations

AOT40:

Accumulated ozone exposure over 40 nmol mol−1

APX:

Ascorbate peroxidase

AsA:

Ascorbate

CAT:

Catalase

Ci:

Intercellular CO2 concentration

EL:

Electrolyte leakage

Gs:

Stomatal conductance

H2O2 :

Hydrogen peroxide

–OH:

Hydroxyl radical

MDA:

Malondialdehyde

OTCs:

Open top chambers

Pn:

Photosynthetic rate

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

O ·−2 :

Superoxide anion radical

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Acknowledgments

This work was funded by the National Natural Science Foundation of China Important Project 90411019, the Foundation of Knowledge Innovation Program of Chinese Academy of Sciences kzcx3-sw-43 and the Innovation Program of Institute of Applied Ecology, Chinese Academy of Sciences SLYQY0414. We express our sincere thanks to Prof. Dali Tao for critical reading of the manuscript.

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  1. Department of Urban Forest, Institute of Applied Ecology, Chinese Academy of Sciences, 110016, Shenyang, People’s Republic of China

    Kun Yan, Wei Chen, Guoyou Zhang, Sheng Xu, Zhouli Liu & Xingyuan He

  2. Graduate School of the Chinese Academy of Sciences, 100049, Beijing, People’s Republic of China

    Kun Yan, Guoyou Zhang & Zhouli Liu

  3. College of Chemical and Life Science, Shenyang Normal University, 110034, Shenyang, People’s Republic of China

    Lanlan Wang

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Correspondence to Xingyuan He.

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Yan, K., Chen, W., Zhang, G. et al. Elevated CO2 ameliorated oxidative stress induced by elevated O3 in Quercus mongolica . Acta Physiol Plant 32, 375–385 (2010). https://doi.org/10.1007/s11738-009-0415-z

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